Antibodies directed against mouse CD31 (PECAM-1) (affinity purified SL-4), raised in rabbit and purified as described elsewhere 20 (link). Rat monoclonal antibodies against mouse CD31 [clone: MEC13.3] (Cat No. 550274), CD45 [30-F11] (Cat No. 550539), CD11b [M1/70] (Cat No. 550282), Mac3 [M3/84] (Cat No. 550292), and VE-cadherin (VEC) [11D4.1] (Cat No. 550548) were purchased from BD Biosciences (San Jose, CA, USA). Rabbit polyclonal antibodies against Ki67 (Cat No. ab15580) were purchased from Abcam (Cambridge, MA, USA). Rabbit monoclonal antibodies against Survivin [71G4B7] (Cat No. #2808), YAP [D24E4] (Cat No. #8418), and phospho-YAP (Ser127) [D9W2I] (Cat No. #13008) (P-YAP) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). A mouse monoclonal antibody against glucose transporter 1 (GLUT-1) [SPM498] (Cat No. ab40084) was purchased from Abcam (Cambridge, MA, USA). Mouse monoclonal antibodies against human CD31 [JC70A] (Cat No. M0823), a CD45 [2B11+PD7/26] (Cat No. M0701) and a monoclonal mouse anti human CD68 were purchased from DAKO. Rabbit polyclonal antibodies against human Ajuba (Cat No. HPA006171) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). A rabbit polyclonal against human GLUT1 was purchased from Cell Marque (Rocklin, CA).
Yap d24e4
Manufactured by Cell Signaling Technology
Sourced in United States
YAP [D24E4] is a rabbit monoclonal antibody that recognizes the Yes-associated protein (YAP). YAP is a transcriptional co-activator that plays a key role in the Hippo signaling pathway, which is involved in the regulation of cell proliferation and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Cd45 2b11 pd7 26, by Agilent Technologies (1 mentions)
Monoclonal mouse anti human cd68, by Agilent Technologies (1 mentions)
Cd11b m1 70, by BD (1 mentions)
Cd45 30 f11, by BD (1 mentions)
Mac3 m3 84, by BD (1 mentions)
Spm498, by Abcam (1 mentions)
Survivin clone 71g4b7, by Cell Signaling Technology (1 mentions)
Cd31 jc70a, by Agilent Technologies (1 mentions)
Ki 67 mib 1, by Agilent Technologies (1 mentions)
Rat anti mouse cd68, by Bio-Rad (1 mentions)
3 protocols using yap d24e4
1
Antibody Characterization for Vascular Research
2
Antibody Characterization for Cell Biology
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Rabbit monoclonal antibodies against survivin (clone: 71G4B7) and YAP (D24E4) were purchased from Cell Signaling Technology (Danvers, MA, USA). A rabbit monoclonal antibody against c‐Myc (MYC) (Y69) was purchased from Abcam (Cambridge, MA, USA). Mouse monoclonal antibodies against CD31 (JC70A) and Ki67 (MIB‐1) were purchased from DAKO (Carpinteria, CA, USA). A mouse monoclonal antibody against β‐actin (AC‐15) was purchased from Abcam.
3
Aortic Tissue Analysis and Immunostaining
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Ascending and abdominal aortas were placed in optimal cutting temperature and sectioned (10-µm thickness/section) in sets of 10 slides serially with 9 sections/slide by using a cryostat. One of the slides was stained with Verhoeff’s Iron Hematoxylin staining and measurements were performed to determine medial thickness and elastin breaks. To quantify medial thicknesses, every section from each slide was measured perpendicular from internal to external elastic lamina. Immunohistochemical staining was performed on AA sections to detect phospho YAP and YAP using the rabbit anti-mouse phospho-YAP (Ser127; D9 W21, catalog No: 13008, 1 : 500) and YAP (D24E4; 1 : 200, catalog No: 8418, 1 : 200) antibodies (Cell Signaling Technology [CST], Danvers, MA, USA). The following reagent was used to detect macrophages: rat anti-mouse CD68 (1 : 200, catalog No. MCA1957; Bio-Rad, Hercules, CA, USA). Immunostaining was performed on formalin-fixed frozen or paraffin-embedded sections, with appropriate negative controls, as described previously.15 (link),22 (link)
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