Oxycodone dosing solutions were prepared by dissolving 6.75 mg, 60 mg, or 150 mg oxycodone (United States Pharmacopeia, North Bethesda, Maryland) in 2 ml sterile water (Lonza, Basel, Switzerland) for PK and combination studies. Diazepam dosing solutions were prepared by dissolving 2 mg, 20 mg, or 200 mg diazepam (United States Pharmacopeia, North Bethesda, Maryland) in 4 ml of a 50:50 solution of PEG400 (J.T. Baker, Allentown, PA) and propylene glycol (Thermo Fisher Scientific, Waltham, Massachusetts) for PK studies; and 20 mg diazepam in 2 ml of a 50:50 solution of PEG400 and propylene glycol for the combination study. All doses were given as oral solutions using an 18 gauge oral gavage needle with a 1 ml syringe for oxycodone; and an 18 gauge oral gavage needle with a 1 ml (for combination study) or 3 ml (for PK studies) syringe for diazepam. The oral route of administration for opioids and other SPDs was chosen as it is the most common clinical route for these prescribed drugs [20 (link),21 (link)].
Peg400
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Belgium
PEG400 is a polyethylene glycol with an average molecular weight of 400 g/mol. It is a clear, viscous liquid commonly used as a solvent, plasticizer, and dispersing agent in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Propylene glycol, by Thermo Fisher Scientific (2 mentions)
Rapamycin, by LC Laboratories (2 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions)
Tween 80, by Thermo Fisher Scientific (2 mentions)
Labrasol, by Gattefosse (2 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
Sucrose, by Thermo Fisher Scientific (1 mentions)
Urea, by Thermo Fisher Scientific (1 mentions)
Glycine, by Thermo Fisher Scientific (1 mentions)
Ethylene glycol, by Thermo Fisher Scientific (1 mentions)
Guanidine hydrochloride, by Thermo Fisher Scientific (1 mentions)
Ficoll, by GE Healthcare (1 mentions)
Peg 2000, by Thermo Fisher Scientific (1 mentions)
Pluronic f 127, by Merck Group (1 mentions)
Cellulose membrane, by Merck Group (1 mentions)
Glycerol, by Thermo Fisher Scientific (1 mentions)
Polyethylene glycol, by Thermo Fisher Scientific (1 mentions)
Hydroxypropyl methylcellulose, by Thermo Fisher Scientific (1 mentions)
Carboxy methylcellulose sodium, by Thermo Fisher Scientific (1 mentions)
31 protocols using peg400
1
Oxycodone and Diazepam Dosing Protocol
2
Synthesis of Sodium-PEG400 Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 4
Polyethylene glycol 400 (PEG-400, Alfa Aesar) represents a polyethylene glycol mixture of average formula H(OC2H4)8.67(OH) (FW=400).
(a) The clear solution of [Na(Et2O)3][H2NB2(C6F5)6] (12.85 g, 10.0 mmol) in 50 mL of dichloromethane is treated with PEG-400 (3.55 mL, 10.0 mmol) and the same volume of pentane is added. In the course of several days colorless needles separate which were analyzed as [Na(PEG-400)][H2NB2(C6F5)6]. Full removal of all volatiles from the mother liquor by vacuum leaves an additional colorless solid of same composition; total yield is quantitative.
(b) The solution of [Na(Et2O)3][H2NB2(C6F5)6] (12.85 g, 10.0 mmol) in 50 mL of dichloromethane is treated with PEG-400 (10.0 mL), 28.2 mmol). All volatiles are removed in a vacuum. The remaining liquid is extracted with 50 mL of pentane and the upper pentane phase is discarded. The lower phase is freed from residual pentane under vacuum to leave a colorless oil which has been analyzed for [Na(PEG-400)≈2.7][H2NB2(C6F5)6]; yield is quantitative. Both the solid and the liquid formulations of [Na(PEG-400)n]+[FAB]− are ready for oral administration to the patient.
3
Synthesis of Highly Stable Carbon Dots
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Synthesis of C-dots with TCB: In a standard recipe, 180 mg of 1,2,4,5-tetracyanobenzene (TCB, ABCR, Karlsruhe, Germany, 98%) were dissolved in 50 mL of polyethylene glycol 400 (PEG400, Alfa Aesar, Karlsruhe, Germany) and heated in a round-bottomed flask under nitrogen atmosphere with a mantle heater to 160 °C. This temperature was maintained for 1 h. The proceeding decomposition of PEG400 and the formation of the C-dots can be followed by the naked eye and is indicated by the color of a yellow solution that slowly turns to a deep black suspension at 100–120 °C. Subsequent to the synthesis of natural cooling to room temperature, the resulting deep black suspensions can be directly diluted with water to obtain colloidally highly stable aqueous suspensions. Alternatively, the C-dots can be separated after addition of 350 mL of propan-2-ol by centrifugation (20,000 r.p.m., 10 min). Thereafter, the C-dots were purified three times by redispersion/centrifugation in/from propan-2-ol to obtain 130 mg of C-dots.
4
Protein Purification Buffer Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sarcosine, PEG400, PEG2000 (Alfa Aesar), Ficoll (GE Healthcare), guanidine hydrochloride (Thermo Fisher Scientific), ethylene glycol, glycine, potassium chloride, sodium chloride, sucrose and urea (Fisher Scientific) were used without further purification. Stock solutions were made by mixing the solute with 20 mM sodium phosphate buffer, pH 7.4, with the addition of 100 mM NaCl except for experiments where the concentration of NaCl or KCl was varied, which began free of additional salt. The same buffer was used for all dilutions.
5
Curcumin Formulation Optimization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Curcumin was supplied from TCI (Tokyo Chemical Industry Co., Ltd. Tokyo, Japan). Hydroxy propyl methylcellulose (HPMC), carboxy methylcellulose sodium (Na CMC), glycerol (GLY), polyethylene glycol (PEG 400), triethylacetate 99% (TEC) were supplied by Alfa Aesar, Heysham, England. Polyvinyl alcohol (PVA) molecular weight (MW) 31kDa–50 kDa, 98–99% hydrolysed, Pluronic F127 and cellulose membrane MW cut-off 12 kDa–14 kDa were obtained from Sigma-Aldrich (St. Louis, MO, USA).
6
Evaluating Paraben Antimicrobial Efficacy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methylparaben was purchased from FAGRON IBERICA (Barcelona, Spain) and PEG-400 from Thermo Fisher Scientific (Barcelona, Spain).
Trypto-Casein Soy Agar (TSA) (Oxoid, Madrid, Spain) and Sabouraud Dextrose Agar (Oxoid, Madrid, Spain) were used as culture media. We used sodium chloride-peptone buffer at pH 7.7 for sample neutralising and as a sterile suspending fluid (Scharlau, Barcelona, Spain). Beerens Diluent 3% was used to neutralise parabens.
Trypto-Casein Soy Agar (TSA) (Oxoid, Madrid, Spain) and Sabouraud Dextrose Agar (Oxoid, Madrid, Spain) were used as culture media. We used sodium chloride-peptone buffer at pH 7.7 for sample neutralising and as a sterile suspending fluid (Scharlau, Barcelona, Spain). Beerens Diluent 3% was used to neutralise parabens.
7
Rapamycin Administration for Neuroprotection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rapamycin injections were administered following the method described by Butler et al (15 (link)). Rapamycin (LC Laboratories, Woburn, MA, USA) was initially dissolved in 100% ethanol (20 mg/ml) and then diluted in a vehicle solution containing 5% Tween-80, 5% PEG400, and 4% ethanol (Thermo Fisher Scientific, Inc., Waltham, MA, USA) dissolved in distilled, deionized water. Rapamycin (3 mg/kg) or vehicle was injected intraperitoneally when the mice regained consciousness following the GCI injury (20–30 min) and the treatment was continued once daily until rats were sacrificed.
8
In Vivo Administration of BAY 60-6583
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BAY 60-6583 (Tocris Bioscience, Minneapolis, MN) was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO). For in vivo administration, fresh solutions of BAY 60-6583 (10%, v/v), 0.9% sodium chloride (NaCl; 50%, v/v; Hospira), and polyethylene glycol 400 (PEG 400; 40%, v/v; Thermo Fisher Scientific, Hampton, NH) were mixed and sterile-filtered through 0.22-μm-pore filter disk, and 100 μl of solution was injected intraperitoneally at mouse weight (1 mg/kg), after 8 weeks of ovariectomy surgery, once every 2 days. A solution comprising DMSO (10%, v/v), 0.9% NaCl (50%, v/v), and PEG 400 (40%, v/v) was injected in animals as vehicle control.
9
Synthesis and Characterization of PEG-Based Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PolyEthylene glycol, PEG 400, was purchased from Thermo Fisher Scientific, Rodano (MI), Italy. Molecular formula: H(OCH2CH2)nOH, n = 8–9 (average), at a 99% purity grade. Isophorone diisocyanate (IPDI) was purchased from EVONIK INDUSTRIES, Essen, Germany at a 95% purity grade. Anhydrous sodium chloride was purchased from Sigma Aldrich-Merk Life Science S.r.l., Milano, Italia and was of analytical grade. Ethylene glycol was purchased from Carlo Erba, Cornaredo Milano, Italy at a purity grade >99%. Silica was purchased from Merck Life Science S.r.l., Milano, Italy at a 90% purity grade and particle size <45 µm. Activated carbon was purchased from Sigma Aldrich-Merk Life Science S.r.l., Milano, Italy at a high purity level and particle size <100 µm. Diesel, unleaded gasoline, and certified waste motor oil were purchased from Sigma Aldrich-Merk Life Science S.r.l., Milano, Italia.
10
Biotin-Silane Nanoparticle Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Biotin and m-PEG-silane (PJK-1915 and PLS-2013) was purchased from Creative PEGWorks (NC). The fluorescently labeled nanoparticles (F-8767, F8768, and F13081), the biotin tagged HSV type 1/2 polyclonal antibody (PA1-7488), biotin tagged S. aureus polyclonal antibody (PA1-73174), PEG-300 and PEG-400 were acquired from Thermo Fischer Scientific. The viral culture (NATtrol Herpes Simplex Virus Type 1 Strain: MacIntyre, 50,000 copies/ml) was purchased from ZeptoMetrix Corporation. The S. aureus samples were bought from ATCC (ATCC 27660). The washing buffer comprised 50 mM phosphate buffer, pH 7.4. Unless otherwise stated, reagents were purchased from Sigma-Aldrich. All the materials were used as received.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!