K sfm kit

The human normal prostate cell line RWPE-1 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in the keratinocyte serum free media kit (K-SFM kit, Invitrogen-GIBCO, Carlsbad, CA, USA) at 37 °C with 5% CO₂. The human PCa cell lines LNCaP and PC-3 were obtained from the Korean cell line bank (KCLB, Seoul, Korea) and cultured in the RPMI-1640 medium with 10% fetal bovine serum and 1% penicillin/streptomycin (WelGene, Daegu, Korea) at 37 °C with 5% CO₂. HUVECs were cultured in M199 medium with 20% fetal bovine serum, 5 U/mL heparin, 3ng/mL basic fibroblast growth factor (R&D Systems, Minneapolis, MN, USA), and 100 U/mL of antibiotic–antimycotic in 0.1% gelatin-coated flasks at 37 °C with 5% CO₂. Apigetrin (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in Dimethyl Sulfoxide (DMSO) and used for in vitro assays and mechanistic studies.

The human PCa cell lines, LNCaP, 22Rv1, VCaP, PC-3, MDA PCa 2b, and C4-2B, and the immortalized prostate epithelial cell lines, RWPE-1 and HPrEC, were purchased from the American Type Culture Collection (ATCC). LNCaP and 22Rv1 cells were cultured in RPMI-1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS, HyClone). VCaP cells were grown in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) supplemented with 10% FBS. PC-3 and MDA PCa 2b cells were cultured in F-12K medium (Invitrogen) supplemented with 10% FBS. C4-2B cells were maintained in T-medium (Invitrogen) supplemented with 10% FBS. RWPE-1 and HPrEC cells were cultured in Keratinocyte Serum Free Medium (K-SFM) kit (Invitrogen). All cell lines were authenticated by short tandem repeat (STR) profiling. The cells were grown in a humidified incubator under 5% CO₂ at 37 °C. All cell lines were tested regularly for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).

The human PCa cell lines, PC-3 and C4-2, and the prostate epithelial cell line RWPE-1 were purchased from the American Type Culture Collection (ATCC). PC-3 and C4-2 cells were cultured in RPMI-1640 medium (Solarbio) containing 10% fetal bovine serum (FBS, HyClone), while RWPE-1 was cultured in a keratinocyte serum-free medium (K-SFM) kit (Invitrogen). All cell lines were tested and authenticated by short tandem repeat (STR) profiling. The cells were grown in a humidified incubator under 5% CO₂ at 37°C.