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Hm 320 rotary microtome

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The HM 320 rotary microtome is a precision instrument used in laboratories for the preparation of thin tissue sections. It features a robust and durable design, allowing for consistent and accurate sectioning of samples. The device incorporates a motorized mechanism that enables controlled and uniform movement of the specimen block during the sectioning process.

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Microtome (21 citations) Rotary microtome (9 citations) HM 320 (2 citations)

2 protocols using hm 320 rotary microtome

1

Lung Tissue Fixation and Histology

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Lung tissues were harvested and inflation fixed in situ with 4 % paraformaldehyde at constant pressure of 10 cm H2O for 10 min with the chest cavity open. Coronal sections were cut from the lungs, embedded in paraffin, and sectioned at a thickness of 5 μm with an HM 320 rotary microtome (Carl Zeiss, Thornwood, NY). Prepared sections were then stained with hematoxylin and eosin (H&E), and histological evaluation was performed. Sample identification was coded to ensure unbiased evaluation.
Shvedova A.A., Kisin E.R., Yanamala N., Farcas M.T., Menas A.L., Williams A., Fournier P.M., Reynolds J.S., Gutkin D.W., Star A., Reiner R.S., Halappanavar S, & Kagan V.E. (2016). Gender differences in murine pulmonary responses elicited by cellulose nanocrystals. Particle and Fibre Toxicology, 13, 28.
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2

Lung Histopathology After Exposure

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Changes in lung morphology were analyzed by a board-certified pathologist at 7 and 28 days post exposure. Lungs were harvested, insufflated with 4% paraformaldehyde, coronal sections were cut from the lungs, embedded in paraffin, sectioned at a thickness of 5 μm with an HM 320 rotary microtome (Carl Zeiss, Thornwood, NY), stained with Masson trichrome, and submitted for histopathology. Slides were assessed for histopathologic changes, including the presence of pigment-laden macrophages, bronchiolar epithelial hyperplasia, peribronchial, perivascular and alveolar fibrosis, and the number of epithelioid granulomas. Sample identification was coded to ensure blinded evaluation.
Khaliullin T.O., Kisin E.R., Murray A.R., Yanamala N., Shurin M.R., Gutkin D.W., Fatkhutdinova L.M., Kagan V.E, & Shvedova A.A. (2017). Mediation of the single-walled carbon nanotubes induced pulmonary fibrogenic response by osteopontin and TGF-β1. Experimental lung research, 43(8), 311-326.
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