Mouse anti bcl 2 sc 7382

High glucose Dulbecco's modified Eagle's medium (DMEM), Roswell Park Memorial Institute 1640 medium (RPMI), fetal bovine serum (FBS), penicillin-streptomycin were obtained from Bioidea (Iran). Nisin, 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO) and 5,5',6,6'-tetrachloro-1,1',3,3'tetraethylbenzimidazolylcarbocyanine iodide (JC-1) were provided from Sigma-Aldrich (USA).
Mouse anti-Bcl-2 (sc-7382), mouse anti-β-actin (sc-47778), mouse anti-Bax (sc-20067), and goat anti-mouse IgG-HRP (sc-2031) were provided from Santa Cruz Biotechnology (USA). All other reagents were purchased from Sigma Aldrich (USA) and were of analytical grade. D r a f t 5

Materials. GM sulfate (G1272) was purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany); C57BL/6J mice were acquired from the Model Animal Research Center of Nanjing University (Nanjing, China); 3-methyladenine (3-MA) was purchased from Sigma-Aldrich; Merck Millipore (M9281) and Dulbecco's modified Eagle's medium (DMEM) was obtained from Gibco; Thermo Fisher Scientific, Waltham, MA, USA. The following primary antibodies were used: Rabbit anti-Atg12 (2011S) and rabbit anti-Bcl-2 (50E3) from Cell Signaling Technology, Inc. (Danvers, MA, USA); rabbit anti-LC3 (L7543; Sigma-Aldrich; Merck Millipore) and mouse anti-Bcl-2 (SC-7382; Santa Cruz Biotechnology, Inc., Dallas, TX, USA). The following secondary antibodies were used: Cyanine (Cy3)-conjugated donkey anti-rabbit (A31572; Thermo Fisher Scientific, Inc.), horseradish peroxidase (HRP)-conjugated goat anti-rabbit (111-035-003; Jackson ImmunoResearch Laboratories, Inc., Westgrove, PA, USA) and IgG HRP-conjugated goat anti-mouse (SC-2005; Santa Cruz Biotechnology, Inc.). Fluorescein isothyanate (FITC)-phalloidin (P5282) and DAPI (D9542) were purchased from Sigma-Aldrich; Merck Millipore, and a Pierce Classic Immunoprecipitation kit was obtained from Thermo Fisher Scientific, Inc. (26146).