Rag2 il2rg mice

All animal experiments were approved by the Gothenburg County Regional Ethical Committee (permit number 126/14 and 2459/19). WT C57BL/6J and C57BL/6JRj mice were purchased from Charles River (Sulzfeld, Germany) and Janvier Labs (Le Genest-Saint-Isle, France), respectively. Rag1^−/− mice and WT C57BL/6 mice, used in some experiments, were obtained from in-house breeding (University of Gothenburg, Sweden). Rag2^−/−Il2rg^−/− mice were purchased from Taconic (Germantown, NJ, USA). Age and sex-matched mice at 7–12 weeks of age were used in all experiments. Mice were housed in pathogen-free conditions and were given food and water ad libitum.

Wild-type mice were purchased from Shanghai SLAC Laboratory Animal Co. Rag1^–/–, Csf2^−/−, Rorc^gfp/gfp, Rorc-cre, Rosa26^stop-YFP, and Thy1.1 mice were purchased from Jackson laboratory. Rag2^−/−Il2rg^−/− mice were purchased from Taconic Biosciences. Mice used for in vivo studies were littermate controlled and were 6–10 weeks old. Both male and female mice were used unless otherwise noted. All mice used in this study are on C57BL/6 background, and were maintained in specific pathogen-free conditions. In DSS-induced colitis, α-CD40-induced colitis and other in vivo experiments, littermate mice were co-housed since weaning and randomly separated into control and treatment groups to avoid variation in distribution of microflora. All mice were fed with a plain commercial diet (Silaikang, Shanghai). Mice were housed in corn-cob-bedding cages in a room with the light–dark cycle (lights on at 6:00 and off at 18:00). All animal experiments were performed in compliance with the guide for the care and use of laboratory animals, and were approved by the institutional biomedical research ethics committee of the Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

Wild-type (WT) mice (C57BL/6) were from The Jackson Laboratory (Bar Harbor, ME). Rag2^−/− and Rag2^−/−Il2rg^−/− mice were from Taconic Farms. CARD9^−/− and Act1^−/− (also known as CIKS^−/−) mice were generated as described (11 (link), 35 (link)). IL-17RA^−/− mice were from Amgen, and IL-23p19^−/− mice were from Genentech. Dectin-1^−/− and Dectin-2^−/− mice on a C57BL/6 background were generated as described (36 (link), 37 (link)). All experiments included age- and sex-matched controls on the C57BL/6 background. Protocols were approved by the University of Pittsburgh Institutional Animal Care and Use Committee or the University of Aberdeen Animal Welfare and Ethical Review Body and the UK Home Office and adhered to guidelines in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health.

Young (2–3 month) and old (19–24 months) female C57BL/6 mice were obtained from National Institute of Aging via Charles River, or purchased from Charles River, or bred in the animal research facility of Albany Medical Center. B6‐LY45.2 (CD45.1) mice were purchased from Charles River. Rag2^‒/‒Il2rg^‒/‒ mice were purchased from Taconic (Model #4111). All animal experiments were performed according to protocols approved by the Institutional Animal Care and Use Committee at Albany Medical Center.