Mrl mpj faslpr j mice

C57Bl/6 (B6) and B6.SJL-H2s (B6.s) mice were obtained from colonies held at the animal facility at the University of Lübeck. Mice were housed under specific pathogen-free conditions and provided standard mouse chow and acidified drinking water ad libitum. Mice aged 6–10 weeks were used for the experiments. All clinical examinations, biopsies, and bleedings were performed under anesthesia with i.p. administration of a mixture of ketamine (100 µg/g) and xylazine (15 µg/g). Evaluation of skin lesions was performed as described (10 (link)). Animal experiments were approved by local authorities of the Animal Care and Use Committee (Kiel, Germany) and performed by certified personnel. For KLH immunization studies, male Crl:CD1 (ICR) mice were purchased from Charles River. Female MRL/MpJ-Faslpr/J mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). Animals were housed in polycarbonate cages, with free access to water and non-purified stock diet and allowed to condition for 2 weeks in their new environment at 22 ± 2°C, 40–70% relative humidity, and 12 h:12 h light:dark cycles. All animal care and experimental procedures followed the European Community Directive 86/609/CEE and the Autonomous Catalan law (Decret 214/1997) for the use of laboratory animals and were approved by the Almirall Animal Experimentation Ethical Committee.

Female, immunocompetent Balb/c mice with EGFP expression under the control of the Foxp3 promoter (#0,006,769), immunodeficient NOD-SCID (NOD.Cg-Prkdc^scid/J) mice (#001,303), and hyperprolactinemic, lupus-prone MRL-lpr (MRL/MpJ-Fas^lpr/J mice (#000,485) were obtained from Jackson Labs, Sacramento, CA. The study was limited to female animals because of the 100:1 ratio of breast cancer in females versus males. All animal experimentation was approved by the University of California Institutional Animal Care and Use Committee.

MRL/MpJ-Fas^lpr/J mice (Stock No. 000485) were obtained from The Jackson Laboratory (Bar Harbor, ME). Mice with the nail abnormalities were discovered by an animal care technician (Susan Swana) in this colony. Mutant mice were removed and bred to establish a stable and separate colony by mating homozygous mutant mice. Mice in the pedigree that produced the mutant animals were observed to ensure this mutation was not segregating within the main production colony, confirming that all affected lineages had been removed.
Mice were maintained at The Jackson Laboratory in a humidity-, temperature-, and light cycle (12:12) controlled vivarium under specific pathogen-free conditions and were allowed free access to autoclaved food (NIH 31, 6% fat; LabDiet 5K52, Purina Mills, St. Louis, MO) and acidified water (pH 2.8–3.2). All work was done with the approval of The Jackson Laboratory Animal Care and Use Committee in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health.

Four-week-old female MRL/MpJ-Fas^lpr/J mice (Jackson Laboratories) were group-housed under climate-controlled conditions with a 12-h light / dark cycle, a standard temperature of 20 ± 3 °C and appropriate environmental enrichment (cardboard, tissues, red polycarbonate houses and seeds) in the cages. Mice had free access to food and drinking water ad libitum.