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Coolsnap ccd camera

Manufactured by Nikon
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Sourced in Japan

The CoolSnap CCD camera is a scientific imaging device designed for high-performance digital microscopy and other lab applications. It features a cooled charge-coupled device (CCD) sensor that provides low-noise, high-resolution image capture. The CoolSnap camera is capable of capturing detailed images for a variety of scientific and research purposes.

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Lab products found in correlation

Eclipse 90i microscope, by Nikon (1 mentions) Es80 epi fluorescence microscope, by Nikon (1 mentions) Eclipse 80i, by Nikon (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Eclipse 800 microscope, by Nikon (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Metamorph imaging software, by Molecular Devices (1 mentions) Widefield ti eclipse inverted microscope, by Nikon (1 mentions)

Close spelling variants of this product

CCD camera (84 citations) CoolSNAP HQ2 CCD camera (27 citations) CoolSnap HQ2 FireWire CCD-camera (7 citations) CoolSNAP MYO-CCD camera (6 citations) CoolSNAP EZ CCD camera (5 citations) Coolsnap camera (3 citations) CoolSNAP HQ CCD camera (3 citations) CoolSnap EZ monochrome CCD camera (2 citations) CoolSNAP DYNO CCD camera (2 citations)

7 protocols using coolsnap ccd camera

1

Microscopic Imaging of Caenorhabditis elegans

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For imaging, animals were anesthetized either with 0.2% levamisole (Govindan et al. 2009 (link)) or with 10 mM NaN3, mounted on 2% agarose pads containing 10 mM NaN3, and imaged using either a 40× or a 60× water-immersion objective with the Nikon Eclipse 90i microscope equipped with a Nikon Coolsnap CCD camera. Z projections (sums of intensity) were obtained using NIS-Elements AR, version 4.0. For expression profiling, animals were cultured for at least two generations at 16° and then were synchronized and allowed to grow to the appropriate stage (L1−L4). Forty animals per larval stage were examined for expression. For dauer stage expression, 10 starvation-induced dauer were imaged.
Chou H.T., Vazquez R.G., Wang K., Campbell R., Milledge G.Z., Walthall W.W, & Johnson C.M. (2015). HES-Mediated Repression of Pten in Caenorhabditis elegans. G3: Genes|Genomes|Genetics, 5(12), 2619-2628.
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2

Visualizing Crz1-mCherry Localization

Cited 2 times
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XW252 (26 (link), 27 (link)) was grown overnight for 16 hours in YPD medium at 30°C shaking and subsequently diluted to an OD600 ~0.1 in fresh YPD medium. The cells were cultivated grown at 25°C to OD600 ~ 0.5. Phenothiazine or DMSO was added to the culture and immediately placed in a water bath at the appropriate temperature for 15 minutes. Samples were fixed in 4% formaldehyde for 15 minutes, harvested by centrifugation and re-suspended in 50 μL of 40 mM potassium phosphate/1.2 M sorbitol buffer. Images were captured using Nikon ES80 epi-fluorescence microscope visualized with a CoolSnap CCD camera and NIS-Elements Software. Quantitative analysis was performed by visually inspecting images for co-localization of Crz1-mCherry with Nop1-GFP. The data represent the mean of at least two biological replicates for which at least 100 cells were counted. Error bars indicate standard deviation.
Montoya M.C., DiDone L., Heier R.F., Meyers M.J, & Krysan D.J. (2017). Antifungal phenothiazines: optimization, characterization of mechanism, and modulation of neuroreceptor activity. ACS infectious diseases, 4(4), 499-507.
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3

Crz1 Nuclear Localization Assay

Cited 1 time
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The effect of small molecules on the temperature-induced nuclear localization of Crz1 was determined as previously described [12 (link)]. Briefly, overnight cultures of strain XW253 containing Crz1p-mCherry and Nop1p-GFP in YPD at 30°C were diluted 1:50 into fresh YPD and mock treated (1% DMSO) or treated with ¼ the MIC of the test compound. The resulting cultures were incubated for an additional 4 hr at either 30°C or 37°C. Samples (1 mL) were harvested by centrifugation, washed with DPBS, re-suspended in DPBS, and imaged using a Nikon ES80 epi-fluorescence microscope equipped with a CoolSnap CCD camera. Images were collected using NIS-Elements Software and processed in PhotoShop. Each condition was imaged on multiple days from independent cultures. For quantitative analysis, at least 100 cells with clearly visible signals for both flurophores were analyzed for each replicate and the percentage of cells with colocalized Crz1p-mCherry and Nop1p-GFP were calculated. Data are presented as mean percentage with error bars representing standard deviation.
Butts A., Martin J.A., DiDone L., Bradley E.K., Mutz M, & Krysan D.J. (2015). Structure-Activity Relationships for the Antifungal Activity of Selective Estrogen Receptor Antagonists Related to Tamoxifen. PLoS ONE, 10(5), e0125927.
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4

Tumor Spheroid Immune Profiling

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Mouse-derived organotypic tumor (MDOT) cultures were established from gently dissociated KP tumors, after which the generated tumor spheroids were mixed with supporting collagen matrix and loaded into three-dimensional AIM glass chambers (AIM Biotech, Singapore) in the presence of complete media containing JQ1 (250nm) and anti-PD-1 (10ug/ml). DMSO served as control. After three days in culture, media was aspirated and chambers were gently washed with 1X PBS prior to immunofluorescent staining. Briefly, purified CD16/32 FCγR blocking reagent was added to chambers for 15 minutes at room temperature followed by Alexa fluor 488 anti-mouse CD3 and Alexa fluor 594 anti-mouse EpCAM for one hour. Then, chambers were washed gently with 1X PBS/2% FBS twice and DAPI (Invitrogen, Carlsbad, CA; 1:1000X) was added and incubated for five minutes followed by two washes with 1X PBS/2%FBS. Images were captured on a Nikon Eclipse 80i fluorescence microscope equipped with CoolSNAP CCD camera and merged images created with NIS elements imaging software. Quantification of CD3+ T cells under each treatment condition was determined from an average of 10–11 fields of view as evaluated by fluorescent microscopy.
Adeegbe D.O., Liu S., Hattersley M.M., Bowden M., Zhou C.W., Li S., Vlahos R., Grondine M., Dolgalev I., Ivanova E.V., Quinn M.M., Gao P., Hammerman P.S., Bradner J.E., Diehl J.A., Rustgi A.K., Bass A.J., Tsirigos A., Freeman G.J., Chen H, & Wong K.K. (2018). BET bromodomain inhibition cooperates with PD-1 blockade to facilitate antitumor response in Kras-mutant non-small cell lung cancer. Cancer immunology research, 6(10), 1234-1245.
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5

Fluorescent Visualization of Spermatozoa

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Mouse and boar spermatozoa were mounted on poly-L lysine coated coverslips and fixed in 2% formaldehyde for 40 min. Non-specific binding was blocked using 5% bovine serum albumin (BSA) for 25 min before incubation in primary antibody overnight at 4 °C. The next day, the coverslips were washed extensively in 1% BSA-PBS before a 40 min incubation with secondary antibody conjugated to a fluorescent marker and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) at room temperature and hidden from light. The coverslips were washed again before being mounted on glass slides using VectaShield mounting medium (Vector Laboratories, Burlingame, CA, USA) and sealed with nail polish. Spermatozoa from mouse and boar were also incubated with our fluorescent inhibitor for 25 min, washed extensively and mounted onto glass slides to visualize the binding pattern. Fluorescence images were taken at the Queen’s University Cancer Research Institute Imaging Centre, using a Quorum Wave Effects spinning disc confocal microscope or at the University of Missouri-Columbia, using a Nikon Eclipse 800 microscope with CoolSnap CCD camera (Nikon, Tokyo, Japan). All images were subsequently analyzed using MetaMorph Imaging Software (Molecular Devices, San Jose, CA, USA).
Hamilton L.E., Zigo M., Mao J., Xu W., Sutovsky P., O’Flaherty C, & Oko R. (2019). GSTO2 Isoforms Participate in the Oxidative Regulation of the Plasmalemma in Eutherian Spermatozoa during Capacitation. Antioxidants, 8(12), 601.
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6

Autophagy Visualization and Quantification

Cited 1 time
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Images were collected with a Nikon ES80 epi-fluorescence microscope
equipped with a CoolSnap CCD camera using NIS-Elements software with
constant exposure settings and processed equivalently in PhotoShop.
ATG8-GFP localization as a reporter for autophagy was as described
by Eisenberg et al.29 (link) The percentage of
cells showing autophagy was based on counting at least 100 cells per
technical replicate, and the experiment was performed on two biological
replicates. Propidium iodide staining was performed as previously
described.29 (link)
Koselny K., Green J., Favazzo L., Glazier V.E., DiDone L., Ransford S, & Krysan D.J. (2016). Antitumor/Antifungal Celecoxib Derivative AR-12 is a Non-Nucleoside Inhibitor of the ANL-Family Adenylating Enzyme Acetyl CoA Synthetase. ACS Infectious Diseases, 2(4), 268-280.
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7

Quantifying Cataract Lens Opacities in Zebrafish

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The presence and proportion of cataract lenses were quantified in coronal (optical) sections at the center of the anterior-posterior axis of 1.5–3 mpf fish. An additional 6 mpf group was analyzed by OCT for the presence of cataracts and relative changes in axial length. For this 6 mpf time-point, the extent of the opacities in the lens was quantified in ImageJ. Binary images of coronal lens sections were created and by automated thresholding, the ratio of opaque pixels was measured. For the ex vivo quantification of the proportion of cataract lenses in the 6 mpf group, a differential interference contrast microscope (Nikon WideField Ti-Eclipse inverted microscope with Coolsnap CCD camera) was used. Lenses were classified as cataractous when the nuclear ring structure was clearly visible.
Quint W.H., Tadema K.C., de Vrieze E., Lukowicz R.M., Broekman S., Winkelman B.H., Hoevenaars M., de Gruiter H.M., van Wijk E., Schaeffel F., Meester-Smoor M., Miller A.C., Willemsen R., Klaver C.C, & Iglesias A.I. (2021). Loss of Gap Junction Delta-2 (GJD2) gene orthologs leads to refractive error in zebrafish. Communications Biology, 4, 676.
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