The frozen hippocampus tissue samples were cleaned by precooling phosphate buffer saline (PBS) and then put into a glass homogenizer to add the corresponding volume of PBS for full grinding. After centrifugation for 10 minutes at 3000 r/min, the supernatant was detected by an insulin ELISA kit provided by Elabscience Biotechnology Co., Ltd (Elabscience, Wuhan, China).
Insulin elisa kit
Manufactured by Elabscience
Sourced in China, United States
The Insulin ELISA kit is a quantitative immunoassay designed for the measurement of insulin levels in various biological samples. It utilizes the Enzyme-Linked Immunosorbent Assay (ELISA) technique to detect and quantify insulin concentrations.
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5 protocols using insulin elisa kit
1
Quantifying Hippocampal Insulin Levels
2
Insulin Concentration Quantification
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Insulin Elisa Kit was purchased from Elabscience (Wuhan, China). Insulin detection was measured according to the Manufacturer’s Protocol.
3
Insulin Assay in Rat Serum
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To assess the function of the rat pancreas, a blood biochemical parameter, namely insulin, was assayed. Rat serum samples were isolated from whole blood sample and subjected to insulin biochemical analysis according to the insulin manufacturer’s instructions (Elabscience® Insulin ELISA kit, Houston, TX, USA).
4
Glycemic Impacts of AlCl3 and NaN3 Exposure
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For each group, the serum
level of glucose was observed using the glucose assay kit (ref no.
1004) through a Microlab 300 chemistry analyzer (ELITech Group) to
estimate the effects of AlCl3 and NaN3 on the
glycemic status. HbA1c and insulin levels in serum were also measured
using their respective Elisa kits, insulin ELISA kit (cat no. INS
5275, Elabscience), and HbA1c ELISA kit (cat no. SG10984, Elabscience)
through a microplate ELISA reader (BIOBASE-EL 10A).
level of glucose was observed using the glucose assay kit (ref no.
1004) through a Microlab 300 chemistry analyzer (ELITech Group) to
estimate the effects of AlCl3 and NaN3 on the
glycemic status. HbA1c and insulin levels in serum were also measured
using their respective Elisa kits, insulin ELISA kit (cat no. INS
5275, Elabscience), and HbA1c ELISA kit (cat no. SG10984, Elabscience)
through a microplate ELISA reader (BIOBASE-EL 10A).
5
Evaluating Metabolic Markers in Neuroinflammation
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Measuring glucose, insulin, and HbA1c levels in neuroinflammation and cardiometabolic disturbance is important because these parameters reflect the status of glucose metabolism and insulin sensitivity. Elevated glucose levels can indicate impaired glucose regulation, while insulin levels provide insights into insulin resistance and pancreatic function. HbA1c, a long-term marker of average blood glucose levels, helps assess glycemic control over time, providing valuable information about the risk of diabetes and its complications in the context of neuroinflammation and cardiometabolic disturbances.
In each group, the serum level of glucose was determined using the glucose assay kit (cat no. 1004, Elabscience) and measured with a Microlab 300 chemistry analyzer (ELITech Group) to assess the effects of metformin and vitamin E on glycemic status. Additionally, HbA1c and insulin levels in the serum were quantified using their respective ELISA kits: the insulin ELISA kit (cat no. INS 5275, Elabscience) and the HbA1c ELISA kit (cat no. SG10984, Elabscience) were utilized, and readings were obtained through a microplate ELISA reader (BIOBASE-EL 10A).
In each group, the serum level of glucose was determined using the glucose assay kit (cat no. 1004, Elabscience) and measured with a Microlab 300 chemistry analyzer (ELITech Group) to assess the effects of metformin and vitamin E on glycemic status. Additionally, HbA1c and insulin levels in the serum were quantified using their respective ELISA kits: the insulin ELISA kit (cat no. INS 5275, Elabscience) and the HbA1c ELISA kit (cat no. SG10984, Elabscience) were utilized, and readings were obtained through a microplate ELISA reader (BIOBASE-EL 10A).
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