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Topscan video tracking software

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TopScan is a video tracking software that captures and analyzes the movement of subjects in a controlled environment. It provides accurate and reliable data on the position, velocity, and trajectory of the tracked objects.

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Lab products found in correlation

Med pc 4 software, by Med Associates (2 mentions) Model cm16 cmpa, by Avisoft (1 mentions) Audition, by Adobe (1 mentions)

10 protocols using topscan video tracking software

1

Social Interaction Test Protocol

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Social interaction (SI) test was performed as previously described after CSDS or SSDS (20 (link)). Briefly, this test was performed in two 2.5-min sessions using an open field arena (42 × 42 × 42 cm) containing an interaction zone with a perforated plastic enclosure and two corner zones. The first no-target session examines time in the interaction zone, where an empty perforated plastic enclosure is located. The second target session examines time in the interaction zone, where a perforated plastic enclosure with an unknown CD1 social target is located. Both sessions were recorded by TopScan video tracking software (CleverSys, Reston, VA, United States) to assess the total time spent in the interaction and corner zones.
Pagliusi M J.r., Franco D., Cole S., Morais-Silva G., Chandra R., Fox M.E., Iñiguez S.D., Sartori C.R, & Lobo M.K. (2022). The BDNF-TrkB Pathway Acts Through Nucleus Accumbens D2 Expressing Neurons to Mediate Stress Susceptible Outcomes. Frontiers in Psychiatry, 13, 854494.
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2

Locomotor Chamber Behavior Tracking

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Locomotor chambers were constructed with black acrylic walls (47.5 cm length × 15.5 cm width × 30 cm height) and smooth matte black flooring. Positioned directly above each chamber were infrared video cameras hooked up to a 16-channel DVR (Swann Communications, Inc., Santa Fe Springs, CA). Locomotion was captured by the cameras and analyzed by Topscan video tracking software (Clever Sys., Inc., Reston, VA; Flagel and Robinson, 2007; Meyer et al., 2012b (link)). Condenser microphones with a flat frequency range up to 250 kHz at 8-bit resolution (Model CM16/CMPA, Avisoft Bioacoustics, Berlin, Germany), were placed 8 cm above each chamber and connected to an UltraSoundGate 416H recorder with four balanced analog inputs (Avisoft Bioacoustics, Berlin, Germany). USV recordings were converted to spectrograms and analyzed by an experimenter in Adobe Audition (Adobe Systems Inc, San Jose, CA).
Tripi J.A., Dent M.L, & Meyer P.J. (2016). Individual differences in food cue responsivity are associated with acute and repeated cocaine-induced vocalizations, but not cue-induced vocalizations. Psychopharmacology, 234(3), 437-446.
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3

Experimental Contingencies and Data Analysis

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For most tasks, MED-PC IV software (Version 4.2, Med Associates) was used to apply the experimental contingencies and to collect data. Cocaine cue preference data (locomotion and side preference) was analyzed using TopScan video tracking software (CleverSys, Reston, VA). In the statistical analyses for all the tests, when the sphericity assumption was violated in conducting repeated-measures ANOVAs, a multivariate test (Wilks’ Ʌ) was used. Significant F values were followed by post hoc comparisons with Bonferroni corrections (0.05/number of comparisons). The level of significance was set at p < 0.05. Effect sizes were calculated using partial eta squared (ηp2), Glass’s δ, or Cohen’s d as appropriate. Statistical analyses were carried out using SPSS Statistics (vers. 27, IBM, Armonk, NY) or Statistica (vers. 13, TIBCO, Palo Alto, CA).
Ishiwari K., King C.P., Martin C.D., Tripi J.A., George A.M., Lamparelli A.C., Chitre A., Polesskaya O., Richards J.B., Woods L.C., Gancarz A., Palmer A.A., Dietz D.M., Mitchell S.H, & Meyer P.J. (2023). Environmental enrichment promotes adaptive responding during tests of behavioral regulation in male heterogeneous stock rats. bioRxiv.
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4

Chronic Social Defeat Stress Resilience

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The chronic social defeat stress (CSDS), social interaction test, and stratification into vulnerable and resilient were completed as previously described (31 (link); 36 ; 37 (link)), using TopScan video-tracking software (CleverSys, Reston, VA).
Jochems J., Teegarden S.L., Chen Y., Boulden J., Challis C., Ben-Dor G.A., Kim S.F, & Berton O. (2014). Enhancement of stress resilience through Hdac6-mediated regulation of glucocorticoid receptor chaperone dynamics. Biological psychiatry, 77(4), 345-355.
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5

Locomotor Activity Monitoring in Mice

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Animals were placed into clear acrylic arenas (68 × 36 × 16 cm) that were divided into four individual cages (34 × 18 × 16 cm) that allowed for physical contact and social interaction through wire grating, as previously described38 (link). Cages were furnished with corncob bedding. Food and water were delivered from the side so as not to interfere with video tracking. Ceiling-mounted video cameras interfaced to computers running TopScan video tracking software (Clever Sys Inc., Reston, VA) were used to continuously measure distance traveled in the cage over 5 days during both the light and dark cycle. Red light not visible to the mice was used for video tracking during the dark cycle. As demonstrated in our previous experiments38 (link), it takes a minimum of 3 days for animals to habituate and acclimatize to the cages. As such, activity in days 4 and 5 (in a 5 day protocol) were found to be reliable and reproducible measures of locomotor activity. Days 4 and 5 were chosen a priori to data collection in the present experiment as reliable measures of locomotor activity.
Rendeiro C., Masnik A.M., Mun J.G., Du K., Clark D., Dilger R.N., Dilger A.C, & Rhodes J.S. (2015). Fructose decreases physical activity and increases body fat without affecting hippocampal neurogenesis and learning relative to an isocaloric glucose diet. Scientific Reports, 5, 9589.
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6

Chronic Social Defeat Stress Paradigm

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D1-Cre, D2-Cre, D1-GFP, and D2-GFP mice underwent 10-day CSDS. This is a well-established protocol that yields stress susceptible (mice displaying depression-like behaviors) or resilient cohorts (1 (link),2 (link),41 (link)). Experimental mice were exposed to an aggressive retired CD1 breeder for 10 minutes per day and then housed within the same cage as the CD1 on the opposite side of a perforated divider to maintain sensory contact. This was repeated for 10 days with a novel CD1 mouse on each day. On day 11, mice were screened in a SI behavior. Animals were placed in an open field with a perforated box located in a designated interaction zone for 2.5 minutes and assessed for time spent in the interaction zone using TopScan video tracking software (CleverSys, Reston, Virginia). Subsequently, a novel CD1 was placed in the perforated box and experimental mice were assessed for time spent interacting with the novel social target in the interaction zone. Mean interaction time was assessed in each experiment. For each experiment, animals were deemed susceptible if interaction times were two standard deviations below the mean of nondefeated control animals or resilient if they did not fall into this category. Mice that received CSDS and optogenetic or pharmacogenetic manipulations underwent a second SI.
Francis T.C., Chandra R., Friend D.M., Finkel E., Dayrit G., Miranda J., Brooks J.M., Iñiguez S.D., O’Donnell P., Kravitz A, & Lobo M.K. (2014). Nucleus Accumbens Medium Spiny Neuron Subtypes Mediate Depression-Related Outcomes to Social Defeat Stress. Biological psychiatry, 77(3), 212-222.
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7

Automated Rodent Behavior Tracking

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Rats were tested in the dark in black acrylic chambers (47 cm length × 19 cm width × 30 cm height) with either “grid” or “hole” textured floors that were spray painted black. Beneath the textured floors was an additional smooth black matte floor. During testing, subjects were video recorded using infrared cameras connected to a 16-channel DVR (Swann Communications, Inc., Santa Fe Springs, CA) to analyze locomotor activity and side/floor preference. Videos were analyzed in real-time using Topscan video tracking software (Clever Sys. Inc., Reston, VA)3 (link),27 (link). All testing environments were located in custom-built light- and sound-attenuating chambers.
King C.P., Tripi J.A., Hughson A.R., Horvath A.P., Lamparelli A.C., Holl K.L., Chitre A.S., Polesskaya O., Ishiwari K., Solberg Woods L.C., Palmer A.A., Robinson T.E., Flagel S.B, & Meyer P.J. (2021). Sensitivity to food and cocaine cues are independent traits in a large sample of heterogeneous stock rats. Scientific Reports, 11, 2223.
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8

Locomotor Activity and Preference in Rats

Cited 1 time
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Rats were tested in black acrylic chambers (47 cm length × 19 cm width × 30 cm height) with black spray-painted textured floors that were either “grid” or “hole”. Textured floors were placed on top of a smooth black matte floor underneath the testing chamber. The entire chamber was spray-painted black to maximize video contrast for video capture via infrared cameras connected to a 16-channel DVR (Swann Communications, Inc., Santa Fe Springs, CA). Locomotor data and side preference were analyzed using Topscan video tracking software (Clever Sys., Inc., Reston, VA; Flagel & Robinson, 2007 (link), Meyer et al., 2012b (link)). Chambers were housed in custom-made light-proof, sound attenuating chambers. Cocaine was prepared by dissolving powder from cocaine HCl (Nat. Inst. Of Drug Abuse, Bethesda, MD) into saline at a concentration of either 10 mg/mL or 20 mg/mL to produce solutions for the 10 mg/kg or 20 mg/kg conditions respectively.
King C.P., Militello L., Hart A., St Pierre C.L., Leung E., Versaggi C.L., Roberson N., Catlin J., Palmer A.A., Richards J.B, & Meyer P.J. (2017). Cdh13 and AdipoQ gene knockout alter instrumental and Pavlovian drug conditioning. Genes, brain, and behavior, 16(7), 686-698.
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9

Quantifying Cocaine Cue Preferences using MED-PC and Video Tracking

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For most tasks, MED-PC IV software (Version 4.2, Med Associates) was used to apply the experimental contingencies and to collect data. Cocaine cue preference data (locomotion and side preference) was analyzed using TopScan video tracking software (CleverSys, Reston, VA). In the statistical analyses for all the tests, when the sphericity assumption was violated in conducting repeated-measures ANOVAs, a multivariate test (Wilks’ Ʌ) was used. Significant F values were followed by post hoc comparisons with Bonferroni corrections (0.05/number of comparisons). The level of significance was set at p < 0.05. Effect sizes were calculated using partial eta squared (ηp2), Glass’s δ, or Cohen’s d as appropriate. Statistical analyses were carried out using SPSS Statistics (vers. 27, IBM, Armonk, NY) or Statistica (vers. 13, TIBCO, Palo Alto, CA). These results are reported in accordance with ARRIVE guidelines (https://arriveguidelines.org).
Ishiwari K., King C.P., Martin C.D., Tripi J.A., George A.M., Lamparelli A.C., Chitre A.S., Polesskaya O., Richards J.B., Solberg Woods L.C., Gancarz A.M., Palmer A.A., Dietz D.M., Mitchell S.H, & Meyer P.J. (2024). Environmental enrichment promotes adaptive responding during tests of behavioral regulation in male heterogeneous stock rats. Scientific Reports, 14, 4182.
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10

Three-Chamber Social Interaction Test for Mice

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The three-chamber social test was performed to investigate social behavior in female mice. This test was performed as previously described (27 (link)). Briefly, this test is performed using a three-chamber arena; each chamber is sized 30.5 × 19.5 × 31.0 cm and is made with clear acrylic Plexiglas. First, the experimental mouse was placed in the center chamber and allowed free exploration in the entire arena (all three chambers) for 5 min. After this first session, our experimental mouse was removed from the arena for 30 s, and an unfamiliar female mouse was placed in one of the side chambers (social chamber) inside a perforated plastic enclosure, while an empty plastic enclosure remained in the opposite side chamber (counterbalanced across experimental mice). Subsequently, the same experimental female mouse was placed in the center chamber for the second session and was allowed for free exploration in the entire arena. Both sessions were recorded by TopScan video tracking software (CleverSys, Reston, VA, United States) to assess the total time spent in the interaction, center, and empty chambers.
Pagliusi M J.r., Franco D., Cole S., Morais-Silva G., Chandra R., Fox M.E., Iñiguez S.D., Sartori C.R, & Lobo M.K. (2022). The BDNF-TrkB Pathway Acts Through Nucleus Accumbens D2 Expressing Neurons to Mediate Stress Susceptible Outcomes. Frontiers in Psychiatry, 13, 854494.
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