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Ir spirit fourier transform infrared spectrophotometer

Manufactured by Shimadzu
Sourced in Japan

The IR Spirit Fourier-Transform Infrared spectrophotometer is a laboratory instrument designed for the analysis of molecular structures using infrared spectroscopy. It measures the absorption of infrared radiation by a sample, providing information about the chemical composition and characteristics of the material.

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4 protocols using ir spirit fourier transform infrared spectrophotometer

1

Spectroscopic Analysis of Botanical Extract

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The extract was diluted to a 1 mg/mL final concentration and used for UV spectra. The UV-visible spectrum was obtained from 190–400 nm using a UV-3600 Plus UV-VIS-NIR Spectrophotometer (Shimadzu, Japan).
The structural-functional group of compounds in the extract was obtained using IRSpirit Fourier Transform Infrared spectrophotometer (Shimadzu, Japan). A drop of the extract was placed on the diamond crystal to form a thin film. The spectral scanning was taken at the frequency range of 4000 to 400 cm−1 at 4 cm−1 at room temperature.
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2

Comprehensive Characterization of Coumarin Probe

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Steady-state fluorescence measurements and UV-Vis spectrophotometric measurements were performed on a Tecan Infinite 200 microplate reader (Tecan Austria GmbH, Grödig/Salzburg, Austria). The NMR spectrum of the coumarin-derived probe was acquired on a Bruker Avance III spectrometer (500 MHz), using DMSOd6 as a solvent. The infrared spectra were recorded in the region of 4000 cm−1 to 400 cm−1 on a Shimadzu IR Spirit Fourier-Transform Infrared spectrophotometer equipped with an ATR adapter. HPLC-ESI-MS analyses were performed on an LCMS-8030 mass spectrometer (Shimadzu, Kyoto, Japan), according to the method described previously [34 (link)]. All HPLC-MS analyses were performed in a positive ion mode.
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3

Physicochemical Characterization of Compounds

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UV-Vis spectrophotometric and steady-state fluorescence measurements were performed on a Tecan Infinite 200 microplate reader (Tecan Austria GmbH, Grödig/Salzburg, Austria).
The FT-IR spectra were recorded in the region of 4000 cm−1 to 450 cm−1 on a Shimadzu IR Spirit Fourier-Transform Infrared spectrophotometer equipped with an ATR adapter (Shimadzu, Kyoto, Japan).
Mass spectrometry analysis was performed on Shimadzu 8030 ESI-Triple Quad mass spectrometer (Shimadzu, Kyoto, Japan).
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4

Comprehensive Nanomaterial Characterization

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UV–vis spectroscopic
analysis was carried out on a SHIMADZU UV-1900i UV–vis spectrophotometer.
Absorption spectra were recorded at a resolution of 1 nm within 200–800
nm. The hydrodynamic size was measured through DLS analysis conducted
on a Malvern (Malvern, UK) Zetasizer Nano ZS instrument. FTIR spectra
were recorded on a SHIMADZU (Tokyo, Japan) IRSpirit Fourier transform
infrared spectrophotometer using a KBr pellet with a scan rate of
approximately 4 cm–1 s–1 at 25
°C. SEM measurements and EDX analysis were conducted on a Hitachi
SU-8000 microscope at accelerating voltages of 10 and 15 kV. TEM measurements
were conducted on a TEM-2100F (JEOL, Tokyo, Japan) field emission
electron microscope. XRD analysis was conducted on a Rigaku SmartLab
spectrometer with Cu-Kα radiation. Magnetic behavior was examined
by a VSM 8604, Lakeshore Crytronics Inc., USA at room temperature.
TGA was carried out on a SHIMADZU (Tokyo, Japan) TGA-50 at a heating
rate of 10 °C min–1 under N2.
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