PSMA-positive LNCaP cells (300265; Cell Lines Service, Eppelheim, Germany) were cultivated in Dulbecco’s modified Eagle medium/Nutrition Mixture F-12 with GlutaMAX (1/1, DMEM-F12, Thermo Fisher Scientific, Darmstadt, Germany) supplemented with 10% fetal bovine serum (Merck KGaA, Darmstadt, Germany) and kept at 37 °C in a humidified 5% CO2 atmosphere. One day (24 ± 2 h) prior to all in vitro experiments, the cultivated LNCaP cells were harvested using a mixture of trypsin/ethylenediamine-tetraacetic acid (0.05%/0.02%) in PBS (Thermo Fisher Scientific, Darmstadt, Germany) and centrifuged at 1300 rpm (ca. 190×g) for 3 min at room temperature (Heraeus Megafuge 16, Thermo Fisher Scientific, Darmstadt, Germany). After centrifugation, the supernatant was disposed and the cell pellet was resuspended in culture medium. Cells were counted with a Neubauer hemocytometer (Paul Marienfeld GmbH & Co. KG, Lauda-Königshofen, Germany) and seeded in 24-well plates. IC50 values were determined by transferring 1.50 × 105 cells/mL per well into 24-well plates, whereas internalization was assessed by transferring 1.25 × 105 cells/mL per well into poly-L-lysine (PLL)-coated 24-well plates.
Lncap cells
Manufactured by Cytion
Sourced in Germany, United States
LNCaP cells are a human prostate cancer cell line derived from a lymph node metastasis. They are widely used in cancer research as a model system for studying prostate cancer.
Automatically generated - may contain errors
Lab products found in correlation
Neubauer hemocytometer, by Marienfeld (2 mentions)
Dmem f12, by Harvard Bioscience (2 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Heraeus megafuge 16, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rwpe 1 cells, by American Type Culture Collection (1 mentions)
Pwr 1e, by American Type Culture Collection (1 mentions)
Nci h660 cells, by American Type Culture Collection (1 mentions)
Fetal calf serum (fcs), by Harvard Bioscience (1 mentions)
Glutamax, by Harvard Bioscience (1 mentions)
Trypsin, by Harvard Bioscience (1 mentions)
4 protocols using lncap cells
1
PSMA-Positive LNCaP Cell Cultivation and Assay
2
Culturing human prostate cell lines
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Human prostate epithelial cell lines [PWR-1E (CRL-11611) and RWPE-1 cells (CRL-11609)] and PCa cell lines [PC-3 (CRL-1435™), DU145 (HTB-81), C4-2 (CRL-3314), 22Rv1 (CRL-2505) and NCI-H660 cells (CRL-5813)] were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and LNCaP cells were purchased from Cell Lines Service (CLS, Eppelheim, Germany). The cells were cultured at 37°C in 5% CO2 in RPMI-1640 medium (GENOM, Hangzhou, China) with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/ml penicillin and 100 U/ml streptomycin (Gibco/Thermo Fisher Scientific). The medium was changed every 8-10 h.
3
PSMA-positive LNCaP Cell Cultivation
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PSMA-positive LNCAP
cells (CLS: 300265;
Cell Lines Service GmbH) were cultivated in Dulbecco modified Eagle
medium/nutrition mixture F-12 (1:1) (DMEM-F12, Biochrom) supplemented
with 10% fetal calf serum (Biochrom) and kept at 37 °C in a humidified
5% CO2 atmosphere. One day (24 ± 2 h) prior to all
in vitro experiments with LNCaP cells, the cultivated cells were harvested
using a mixture of trypsin/ethylendiaminetetraacetate (0.05%/0.02%)
in phosphate-buffered saline (PBS) and centrifuged. After centrifugation,
the supernatant was disposed and the cell pellet was resuspended in
culture medium. Afterward, cells were counted with a hemocytometer
(Neubauer) and seeded in 24-well plates. IC50 values were
determined by transferring 150 000 cells/mL per well into 24-well
plates, whereas internalization was assessed by transferring 125 000
cells/mL into 24-well PLL-coated plates.
cells (CLS: 300265;
Cell Lines Service GmbH) were cultivated in Dulbecco modified Eagle
medium/nutrition mixture F-12 (1:1) (DMEM-F12, Biochrom) supplemented
with 10% fetal calf serum (Biochrom) and kept at 37 °C in a humidified
5% CO2 atmosphere. One day (24 ± 2 h) prior to all
in vitro experiments with LNCaP cells, the cultivated cells were harvested
using a mixture of trypsin/ethylendiaminetetraacetate (0.05%/0.02%)
in phosphate-buffered saline (PBS) and centrifuged. After centrifugation,
the supernatant was disposed and the cell pellet was resuspended in
culture medium. Afterward, cells were counted with a hemocytometer
(Neubauer) and seeded in 24-well plates. IC50 values were
determined by transferring 150 000 cells/mL per well into 24-well
plates, whereas internalization was assessed by transferring 125 000
cells/mL into 24-well PLL-coated plates.
4
Cultivation of PSMA-positive LNCaP Cells
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PSMA-positive LNCaP cells (300265; Cell Lines Service) were cultivated in Dulbecco modified Eagle medium (DMEM)/Nutrition Mixture F-12 with GlutaMAX (1:1, DMEM-F12, Biochrom) supplemented with fetal bovine serum (10%, FBS Zellkultur) and kept at 37°C in a humidified CO2 atmosphere (5%). A mixture of trypsin and ethylenediaminetetraacetic acid (0.05%, 0.02%) in PBS (Biochrom) was used to harvest cells. Cells were counted with a Neubauer hemocytometer (Paul Marienfeld).
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