Goat anti rabbit igg h l highly cross adsorbed secondary antibody alexa fluor 568

The transfected cells were cultured in 48-well plates at a density of 6×10⁴ cells/well for 24 h. Following fixation with 4% paraformaldehyde at room temperature for 30 min, the cells were permeabilized with 1% Triton X-100 at room temperature for 15 min followed by blocking with 3% BSA (cat. no. 4240GR100; Biofroxx; neoFroxx) at 37°C for 1 h. After cell incubation with primary antibodies at 4°C overnight, the cells were treated with the corresponding secondary Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (Thermo Fisher Scientific, Inc.) at room temperature for 30 min in the dark. Nuclei were counterstained with DAPI (5 mg/ml; cat. no. C1006; Beyotime Institute of Biotechnology) at room temperature for 20 min in the dark. The stained cells were observed under a fluorescence microscope (magnification, ×200). The antibodies used for immunofluorescence are listed in Table III.