Peroxidase conjugated goat anti rabbit immunoglobulin igg h l

CdCl₂ was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China), with a purity of >98.0%. Alanine/aspartate aminotransferase (ALT/AST) kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). T-PER tissue extraction reagent, NE-PER nuclear and cytoplasmic extraction reagents, SuperSignal West Pico PLUS Substrate kit, and Pierce BCA Protein Assay Kit were obtained from ThermoFisher Scientific (Waltham, MA, USA). Murine IL-1β and IL-6 ELISA kits were purchased from Neobioscience (Shenzhen, China). The antibodies that were used for immunoblotting anti-Keap1, -p38, -phospho-p38, -ERK, -phospho-ERK, -JNK, -phospho-JNK, -NLRP3, -NF-κB p65, and -phospho- NF-κB p65 were purchased from Cell Signaling Technology (Danvers, MA, USA) (1:1000 dilutions). The antibodies against Nrf2 and HO-1 were all purchased from Santa Cruz (Santa Cruz, CA, USA) (all 1:200 dilutions). Antibodies against GAPDH were purchased from Abways Technology (Shanghai, China) (1:2000 dilutions). Peroxidase-conjugated goat anti-rabbit immunoglobulin IgG (H + L), anti-mouse IgG (H + L), and anti-goat IgG (H + L) were obtained from Proteintech Group (Wuhan, China). Other reagents, unless indicated, were obtained from Sigma Chemical Co. (St. Louis, MO, USA).

AS-IV and APAP were obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China), and the purity of the two kinds of reagents is >98.0%. Kits used for detecting ALT, AST, GSH, MDA and SOD were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). NE-PER nuclear and cytoplasmic extraction reagents, T-PER tissue extraction reagent, SuperSignal West Pico PLUS Substrate kit and Pierce BCA Protein Assay Kit were obtained from ThermoFisher Scientific (Waltham, MA, USA). ELISA kits for murine TNF-α, IL-1β, and IL-6 were obtained from Neobioscience (Shenzhen, China). The antibodies used for immunoblotting anti-Keap1 was purchased from Cell Signaling Technology (Danvers, MA, USA) (1:1000 dilutions). Antibodies used for immunoblotting including anti-Nrf2, -HO-1, and -NQO1 were all bought from Santa Cruz (Santa Cruz, CA, USA) (all 1:200 dilutions). Antibodies used for immunoblotting including anti-Actin and -Lamin B were purchased from Proteintech Group (Wuhan, China) (1:2000 dilutions for Actin, 1:5000 dilutions for Lamin B). Peroxidase-conjugated goat anti-rabbit immunoglobulin IgG (H + L), anti-mouse IgG (H + L) and anti-goat IgG (H + L) were obtained from the Proteintech Group (Wuhan, China). Other reagents, unless indicated, were obtained from Sigma Chemical Co. (St. Louis, MO, USA).