Olmesartan medoxomil

The experimental protocol was approved by the Animal Care Regulations (ACR) Committee of Chonnam National University Medical School (CNU IACUC-H-2018-21, date of approval 20-05-2018). Wild-type and Col4a3^–/– mice [5 (link)] on a congenic 129X1/SvJ background were purchased from the Jackson Laboratory (Bar Harbor, ME, USA), and only male mice were used in this study. Olmesartan treatment began at four weeks of age, continued for three weeks, where olmesartan medoxomil (Sigma-Aldrich, St. Louis, MO, USA) at a dose of 5 mg/kg/day dissolved in drinking water was administered; only vehicle was supplied for the other experimental groups. Mice were sacrificed at seven weeks of age.

The adult zebrafish were housed in 1-L tanks separated based on what compound they receive and the treatment group (group 1) and treatment plus discontinuation group (group 2). For each group, three aged males and females between 2–3 years old were selected to control for sex and age distribution per compound (or vehicle control). Each tank was filled with 500 mL of system water along with the dissolved compounds. All samples were treated with a concentration of 10 μM olmesartan medoxomil, captopril, aliskiren hemifumarate, or amlodipine besylate. The vehicle control was treated with 0.2% DMSO. The tanks were changed to fresh system water and compounds daily. The compounds olmesartan medoxomil, captopril, aliskiren hemifumarate, amlodipine besylate were obtained from Sigma-Aldrich (cat #144,689–63-4, 62,571–86-2, 173,334–58-2, A5605).