Anti p53 imx25

Manufactured by Leica

Sourced in United Kingdom

Anti-p53 (IMX25) is a lab equipment product designed for research applications. It is an antibody that specifically binds to the p53 protein, which is a key regulator of cellular processes. The core function of this product is to provide a tool for detecting and studying the p53 protein in biological samples.

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Lab products found in correlation

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Close spelling variants of this product

Anti-p53 (14 citations) IMX25 (2 citations)

2 protocols using anti p53 imx25

Western Blot Analysis of Cellular Protein Markers

Cited 1 time

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Cells were lysed in RIPA buffer (20 mM HEPES pH7, 150 mM NaCl, 1% deoxycholate, 1% Nonidet P-40, 0.1% SDS, 1 mM Na₂VO₄, 2 mM EDTA, and a complete protease inhibitor mixture; Roche). Samples were resolved on NuPage Novex Bis-Tris gels (Invitrogen) and blotted with the following antibodies: anti-p53 (IMX25; Leica Microsystems), anti-Rpl22l1, anti-EIF2α (5324S, Cell Signaling), anti-phospho-EIF2α (9721S, Cell Signaling), anti-calnexin(31 (link)), and anti-GAPDH (6C5; Millipore) (32 (link)).

Fahl S.P., Sertori R., Zhang Y., Contreras A.V., Harris B., Wang M., Perrigoue J., Balachandran S., Kennedy B.K, & Wiest D.L. (2022). Loss of ribosomal protein paralog Rpl22-Like1 blocks lymphoid development without affecting protein synthesis. Journal of immunology (Baltimore, Md. : 1950), 208(4), 870-880.

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Western Blot Analysis of Pro-B Cells

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Cultured pro-B cells were lysed in RIPA buffer (20 mM HEPES [pH 7.0], 150 mM NaCl, 1% deoxycholate, 1% NP-40, 0.1% SDS, 1 mM Na₂VO₄, 2 mM EDTA, and a complete protease inhibitor cocktail; Roche, Basel, Switzerland). Samples were resolved on NuPage Novex Bis-Tris gels (Invitrogen) and blotted with the following antibodies: anti-p53 (IMX25; Leica Microsystems, Newcastle Upon Tyne, UK), anti-Rpl22 (19 (link)), and anti-GAPDH (6C5; Millipore, Billerica, MA).

Fahl S.P., Harris B., Coffey F, & Wiest D.L. (2015). Rpl22 loss impairs the development of B lymphocytes by activating a p53-dependent checkpoint. Journal of immunology (Baltimore, Md. : 1950), 194(1), 200-209.

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