An MMP assay kit (Beyotime Biotech, Shanghai, China) with fluorescent probe JC-1 was used to measure MMP of the hippocampal mitochondria. Incubation of the hippocampal mitochondria suspensions (0.5 mg protein/ml) was carried out with JC-1 reagent at 37°C for 15 min. The red fluorescence was measured at the excitation and emission wavelength of 525 and 590 mm, and green fluorescence was measured at the excitation and emission wavelength of 490 and 530 mm with the Perkin Elmer LS-50B Luminescence fluorescence spectrophotometer. The level of MMP was calculated by the ratio of red fluorescence to green fluorescence.
Ls 50b luminescence fluorescence spectrophotometer
Manufactured by PerkinElmer
Sourced in Germany, United States
The LS-50B Luminescence fluorescence spectrophotometer is a high-performance instrument designed for accurate and reliable fluorescence measurements. It features a high-intensity, long-life xenon discharge lamp as the excitation source, providing a wide range of excitation wavelengths. The instrument is capable of recording emission spectra, excitation spectra, and time-resolved fluorescence measurements, making it a versatile tool for various applications in the field of fluorescence spectroscopy.
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Lab products found in correlation
3 protocols using ls 50b luminescence fluorescence spectrophotometer
1
Hippocampal Mitochondrial Membrane Potential
2
Absorption and Fluorescence Spectroscopy of Plastids
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Ambient temperature absorption spectra of dark acclimated plastids/cells were determined in 1 ml WB (see Table 1 ) using an Ultrospec 8000 Spectrophotometer (GE healthcare). Relative absorbance was recorded at wavelength ranging from 350 to 750 nm with a bandwidth set to 1 nm. Fluorescence emission spectra were measured with a LS50B luminescence/fluorescence spectrophotometer using an excitation wavelength of 440 nm (Perkin Elmer; Hamburg, Germany). Plastids/cells corresponding to 20 �g Chl a were diluted in a total volume of 1 ml WB (see Table�1 ). Scan speed was kept at constant 150 nm/min.
3
Quantifying Hippocampal Mitochondrial ROS
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A ROS assay kit (Beyotime Biotech, Shanghai, China) with fluorescent probe DCFH-DA was used to measure the ROS level in the hippocampal mitochondria. Incubation of the hippocampal mitochondria suspensions (0.5 mg protein/ml) was carried out with 1.6 μM 2′, 7′-dichlorofluorescin diacetate (DCFH-DA) at 37°C for 10 min. The fluorescence was measured with the Perkin Elmer LS-50B Luminescence fluorescence spectrophotometer (California, USA) at the excitation and emission wavelength of 488 and 525 mm, respectively. Then, the mean fluorescence intensities in excitation/emission were quantified and compared.
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