Bacterial culture was diluted appropriately with pure water when required, and cells were removed from the culture medium by centrifugation. The supernatant was passed through an Amicon Ultra-0.5 ml 3 K Centrifugal Filter (Millipore, Billerica, MA, USA). The eluent was subjected to high-performance liquid chromatography (LaChrom Elite: HITACHI High Technologies, Tokyo, Japan) with refractive index detection using a COSMOSIL Sugar-D column (4.6 × 250 mm) (nacalai tesque, Kyoto, Japan) and maintained at 25 °C with a flow of acetonitrile/water (80/20) at 2 ml/min. The retention time was used to identify the stereoisomers, and refractive index units were used to calculate their concentrations.
Amicon ultra 0.5 ml 3 k centrifugal filter
Manufactured by Merck Group
Sourced in United States
The Amicon Ultra-0.5 ml 3 K Centrifugal Filter is a laboratory equipment product designed for sample concentration and buffer exchange. It is a centrifugal filter device with a 3 kDa molecular weight cut-off membrane.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using amicon ultra 0.5 ml 3 k centrifugal filter
1
HPLC Analysis of Bacterial Metabolites
2
Ultracentrifugation Supernatant Concentration
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