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6–8 weeks old (Age), 20–25 gram (Weight) female (Sex) mice were used for in vivo efficacy, imaging and safety studies. 4–6 weeks old (Age), 20–24 gram (Weight), randomized male/female (Sex) mice were used for serum half-life assays. Tumor xenografts live animal imaging, and liver ELISA studies with human cancer cells were carried out using immunodeficient Balb/c derived athymic Nude Foxn1^nu/Foxn1⁺ (Envigo) mice model carrying functional B cell and NK (innate immunity) cells. Randomly selected and weight matched male and female Crl:CD1(ICR) mice (Charles River), a well establish strain for pharmacokinetics studies, were used for serum half-life studies. For surrogate animal studies female (Sex) 6–8 weeks-old (Age) C57BL/6J mice, 22–26 gram (Weight) were used for investigating liver toxicity, detailed tissue distribution, H&E staining, AST/ALT assays, and surrogate in vivo efficacy using MD5–1, muBaCa and chiBaCa antibodies as indicated in Figure legends. For assessing plat resistant patient derived xenografts, female (Sex) SCID C.B-17/IcrHsd-Prkdc (Envigo, Dublin, VA) mice that were 6–8 weeks old (Age) with 20–25 grams were used. Procedures involving animals handling, tumor xenografts and serum half-life studies were reviewed and approved by the Institutional Animal Care and Use Committee here at the University of Virginia and conform to the relevant regulatory standards.