Sulphatase

Manufactured by Merck Group

Sourced in China, United States, Canada

Sulphatase is a laboratory equipment designed for the analysis and quantification of sulphate-containing compounds. It is a crucial tool in various scientific fields, including biochemistry, environmental analysis, and clinical diagnostics. The core function of Sulphatase is to measure the presence and concentration of sulphate ions in a given sample.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using sulphatase

Quantifying Glucosinolates in Arabidopsis

Check if the same lab product or an alternative is used in the 5 most similar protocols

35S::BoLSU1, 35S::BoLSU2 and wild-type Arabidopsis seedlings were treated with sulfur deficiency for 7 d. Then, 150 mg seedlings were harvested for glucosinolate purification with reference to previous descriptions [37 (link)]. GSLs were extracted with 5 mL 80% pre-cooled methanol and the extraction passed through DEAE sephadex columns followed by sulphatase (Sigma, Shanghai, China) treatment. Sinigrin (Sigma, Shanghai, China) was used as an external standard. Extract solutions (5 μL) were subjected into Ultra-high-performance Liquid Chromatography (UPLC) (Agilent, 1290 infinity II) and GSLs were separated on ACQUITY UPLC^® HSS T3 (2.1 × 50 mm, 1.8 μm; Waters). GSL concentrations were normalized to fresh weight.

Yang S., Zhou Z., Zhang T., Zhang Q., Li R, & Li J. (2023). Overexpression of BoLSU1 and BoLSU2 Confers Tolerance to Sulfur Deficiency in Arabidopsis by Manipulating Glucosinolate Metabolism. International Journal of Molecular Sciences, 24(17), 13520.

+ Open protocol

+ Expand

Comprehensive Enzyme Assay Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellulase, chitinase, chitosanase (25.9 U/mL), drieselase, β-glucosidase, β-glucuronidase (140 U/mL), hyaluronidase, laminarinase, lysozyme, lyticase, macerozyme, pectinase (3,000 U/mL), pectolyase, sulphatase (3.37 mg/mL), and trypsin were purchased from Sigma Aldrich (St. Louis, MO). Zymolyase (10 mg/mL) was purchased from ZymoResearch (Irvine, CA). macerozyme was purchased from RPI corp (Mt Prospect, IL). Stock concentrations of enzymes were 20 mg/mL unless otherwise noted.

Noel E.A., Weeks D.P, & Van Etten J.L. (2021). Pursuit of chlorovirus genetic transformation and CRISPR/Cas9-mediated gene editing. PLoS ONE, 16(10), e0252696.

+ Open protocol

+ Expand

Urine Metabolite Extraction and Enrichment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Organic extracts from the urine samples were prepared as described previously (Kato et al. 2004b ). In brief, urine aliquots were thawed and filtered to remove urothelial cells. The volume of each sample was recorded, and the urine was enzymatically de-conjugated in 0.2-M (10% v/v) sodium acetate buffer (pH 5.0) (Sigma, St. Louis, MO, USA) containing β-glucuronidase (6 units/ml urine; Sigma, St. Louis, MO, USA) and sulphatase (2 units/ml urine, Sigma, St. Louis, MO, USA) for 16 h at 37°C. The de-conjugated urinary metabolites were then extracted and concentrated by pouring the urine through two C-18 silica-gel columns stacked in tandem (Waters Sep-Pak WAT04305, Milford, MA, USA). The eluted urine was discarded, and a new tube was placed under the column to collect the organics, which were eluted by pouring 10 ml of methanol through the column. The methanol extract was filtered through a 0.22-μm filter and then solvent-exchanged with dimethyl sulfoxide (DMSO) to produce an organic concentrate at 150×. These extracts were stored at 4°C until mutagenicity assays were conducted.

Adetona A.M., Martin W.K., Warren S.H., Hanley N.M., Adetona O., Zhang J.(., Simpson C.D., Paulsen M.H., Rathbun S.L., Wang J.S., DeMarini D.M, & Naeher L.P. (2019). Urinary Mutagenicity and other Biomarkers of Occupational Smoke Exposure of Wildland Firefighters and Oxidative Stress. Inhalation toxicology, 31(2), 73-87.

+ Open protocol

+ Expand

Urine Metabolite Extraction for Mutagenicity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Coded urine samples (~50ml) were thawed and filtered to remove urothelial cells. The exact volume of each sample was recorded, and the samples enzymatically deconjugated in 0.2M (10% v/v) sodium acetate buffer (pH 5.0) (Sigma–Aldrich Canada, Oakville, Ontario, Canada), containing β-glucuronidase (6 units/ml urine; Sigma–Aldrich Canada) and sulphatase (2 units/ml urine; Sigma–Aldrich Canada) for 16h at 37°C. Deconjugated urinary metabolites were then concentrated using solid-phase extraction on C₁₈ silica (Avantor Performance Materials, Center Valley, PA, USA) with methanol elution (EMD Chemicals, Billerica, MA, USA) as described previously (21 (link),28 (link)). The resulting extracts were reconstituted in dimethylsulphoxide (DMSO; Sigma–Aldrich Canada) to produce urine extracts suitable for mutagenicity assessment. Extracts were stored at 4°C until use.

Long A.S., Lemieux C.L., Yousefi P., Ruiz-Mercado I., Lam N.L., Orellana C.R., White P.A., Smith K.R, & Holland N. (2014). Human urinary mutagenicity after wood smoke exposure during traditional temazcal use. Mutagenesis, 29(5), 367-377.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!