Lps tlr4
LPS (TLR4) is a laboratory product that functions as a ligand for the Toll-like receptor 4 (TLR4). TLR4 is a pattern recognition receptor that binds to lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The interaction between LPS and TLR4 is a key step in the innate immune response to bacterial infection.
Lab products found in correlation
2 protocols using lps tlr4
Toll-like Receptor Signaling in Inflammation
Standardized Cytokine Profiling Assay
For the intracellular cytokine staining (ICS), 10 µg/ml of brefeldin A (final concentration -Sigma-Aldrich) was added to each well, and samples were incubated for 6h at 37°C in 5% CO2, then treated with 2 mM EDTA (final concentration) for 10 min at 37°C. The cells were collected and resuspended in BD FACS Lysing Solution, placed into fresh tubes, and stored at -80°C.
For multiplex analysis, samples were incubated with whole blood for 24h at 37°C in 5% CO2.
The supernatant was collected and stored at -80°C.
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