The following primary antibodies were used: mouse anti–EEA-1 (610457, BD Transduction Laboratories, San Jose, CA); rat anti–LAMP-1 (1D4B; Developmental Studies Hybridoma Bank [DSHB, Iowa City, IA]); rat anti–LAMP-2 (ABL-93; DSHB); rabbit anti-Rab20 (11616-1-AP; Proteintech, Chicago, IL); rabbit anti-Rab20 (GTX119559; GeneTex, Irvine, CA); mouse anti–β-actin antibody (A5316; Sigma-Aldrich, Steinheim, Germany), rabbit anti-GADPH (Sigma-Aldrich), and rabbit anti-EGFR antibody (1005; Santa Cruz Biotechnology, Dallas, TX). Secondary antibodies conjugated with Alexa Fluor 488, 546, or 633 for indirect immunofluorescence studies were from Molecular Probes (Invitrogen). Secondary antibodies conjugated with horseradish peroxidase were obtained from Jackson ImmunoResearch Laboratories and Promega. Unconjugated rabbit anti-rat used for immunogold labeling was from Rockland.
Rabbit anti egfr antibody
Manufactured by Santa Cruz Biotechnology
Rabbit anti-EGFR antibody is a primary antibody that specifically recognizes the Epidermal Growth Factor Receptor (EGFR) protein. It is produced in rabbits and can be used for various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the expression and localization of EGFR in biological samples.
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Lab products found in correlation
Rat anti lamp 1 1d4b, by Developmental Studies Hybridoma Bank (1 mentions)
Rabbit anti rab20 11616 1 ap, by Proteintech (1 mentions)
Rabbit anti rab20 gtx119559, by GeneTex (1 mentions)
Mouse anti β actin antibody a5316, by Merck Group (1 mentions)
Rabbit anti gadph, by Merck Group (1 mentions)
Mouse anti eea1, by BD (1 mentions)
Amersham ecl prime western blotting detection reagent, by GE Healthcare (1 mentions)
2 protocols using rabbit anti egfr antibody
1
Immunofluorescence Analysis of Subcellular Markers
2
EGF-Induced Phosphorylation of EGFR
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In cells stimulated with EGF for 0, 5, 30 min at 37°C, threonine and tyrosine phosphorylation of the wt and mutant T654A proteins was detected by western blotting using rabbit anti-pT654 (ab75986; Abcam, Cambridge, UK) and rabbit anti-pY1086 antibody (#4407; Cell Signaling Technology, Danvers, MA), respectively. Rabbit anti-EGFR antibody (#sc-03; Santa Cruz Biotechnology, Dallas, TX) was used to detect protein expression. After being resolved by SDS-polyacrylamide gel electrophoresis (PAGE), the electrophoresed proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane and incubated with each antibody (primary antibody) and then with a horseradish peroxidase (HRP)-linked anti-rabbit IgG (secondary antibody; 7076, Cell Signaling Technology). Immunoreactive proteins were detected with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) using an ImageQuant LAS 500 device (GE Healthcare).
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