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Vacutainer lithium heparinn lh 170 1 u plus blood collection tubes

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The BD Vacutainer® Lithium Heparin (LH) 170 I.U. Plus Blood Collection Tubes are designed for the collection and transportation of blood samples. They contain lithium heparin as an anticoagulant.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) 2100 bioanalyzer, by Agilent Technologies (2 mentions) Anti human cd4 microbeads, by Miltenyi Biotec (2 mentions) Ms column, by Miltenyi Biotec (2 mentions) Rnalater, by Thermo Fisher Scientific (2 mentions) Rnaase free dnase set, by Qiagen (2 mentions) Lymphoprep, by STEMCELL (2 mentions) Dnase max kit, by Qiagen (2 mentions) Rna 6000 nano kit, by Agilent Technologies (2 mentions) Qubit 4 fluorometer, by Thermo Fisher Scientific (2 mentions) Qubit rna hs assay kit, by Thermo Fisher Scientific (2 mentions) Ficoll paque plus, by GE Healthcare (1 mentions)

Spelling variants of this product

BD Vacutainer (1960 citations) Vacutainer tubes (1123 citations) Blood collection tubes (47 citations) BD Vacutainer Plus (29 citations) BD Lithium HeparinN Vacutainer tubes (3 citations) BD Vacutainer LH (3 citations) Plus Blood Collection Tubes (2 citations)

3 protocols using vacutainer lithium heparinn lh 170 1 u plus blood collection tubes

1

PBMC Isolation from Whole Blood

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Approximately 13 mL of blood was collected per volunteer, per time point, in BD Vacutainer® Lithium HeparinN (LH) 170 I.U. Plus Blood Collection Tubes (BD Biosciences, San Jose, CA, USA). Blood was layered over Ficoll‐Paque™ PLUS (GE Healthcare, Little Chalfont, Buckinghamshire, UK) to isolate peripheral blood mononuclear cells (PBMCs). PBMCs were counted using a hemocytometer.
Ng S.S., Souza‐Fonseca‐Guimaraes F., Rivera F.D., Amante F.H., Kumar R., Gao Y., Sheel M., Beattie L., Montes de Oca M., Guillerey C., Edwards C.L., Faleiro R.J., Frame T., Bunn P.T., Vivier E., Godfrey D.I., Pellicci D.G., Lopez J.A., Andrews K.T., Huntington N.D., Smyth M.J., McCarthy J, & Engwerda C.R. (2018). Rapid loss of group 1 innate lymphoid cells during blood stage Plasmodium infection. Clinical & Translational Immunology, 7(1), e1003.
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2

PBMC Isolation and RNA Extraction

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Blood was collected from VL patients and ECs into BD Vacutainer® Lithium HeparinN (LH) 170 I.U. Plus Blood Collection Tubes (BD Biosciences). Blood was layered over Lymphoprep (StemCell Technologies, Inc.) to isolate PBMCs. CD4+ T cells were isolated from PBMCs by magnetic-activated cell sorting (MACS) using MS Columns and anti-human CD4 MicroBeads (both by Miltenyi Biotec, Bergisch Gladback, Germany) into RNAlater (Invitrogen, Carlsbad, CA). Cells were homogenized using the QIAshredder and RNA isolated using the RNeasy Mini Kit (both by QIAGEN, Hilden, Germany) according to manufacturer’s instructions. DNA digestion was performed using either the RNAase-free DNase set or DNase Max kit (both by QIAGEN). RNA was quantified using the Qubit RNA HS Assay kit on a Qubit 4 Fluorometer (Thermo Fisher, Waltham, MA). The quality of RNA was determined using the RNA 6000 Nano kit, run on a 2100 Bioanalyzer (both by Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions where an RNA Integrity Number (RIN) value of above 8 was optimal.
Kumar R., Bunn P.T., Singh S.S., Ng S.S., de Oca M.M., De Labastida Rivera F., Chauhan S.B., Singh N., Faleiro R.J., Edwards C.L., Frame T.C., Sheel M., Austin R.J., Lane S.W., Bald T., Smyth M.J., Hill G.R., Best S.E., Haque A., Corvino D., Waddell N., Koufariotis L., Mukhopadhay P., Rai M., Chakravarty J., Singh O.P., Sacks D., Nylen S., Uzonna J., Sundar S, & Engwerda C.R. (2020). Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell reports, 30(8), 2512-2525.e9.
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3

PBMC Isolation and RNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood was collected from VL patients and ECs into BD Vacutainer® Lithium HeparinN (LH) 170 I.U. Plus Blood Collection Tubes (BD Biosciences). Blood was layered over Lymphoprep (StemCell Technologies, Inc.) to isolate PBMCs. CD4+ T cells were isolated from PBMCs by magnetic-activated cell sorting (MACS) using MS Columns and anti-human CD4 MicroBeads (both by Miltenyi Biotec, Bergisch Gladback, Germany) into RNAlater (Invitrogen, Carlsbad, CA). Cells were homogenized using the QIAshredder and RNA isolated using the RNeasy Mini Kit (both by QIAGEN, Hilden, Germany) according to manufacturer’s instructions. DNA digestion was performed using either the RNAase-free DNase set or DNase Max kit (both by QIAGEN). RNA was quantified using the Qubit RNA HS Assay kit on a Qubit 4 Fluorometer (Thermo Fisher, Waltham, MA). The quality of RNA was determined using the RNA 6000 Nano kit, run on a 2100 Bioanalyzer (both by Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions where an RNA Integrity Number (RIN) value of above 8 was optimal.
Kumar R., Bunn P.T., Singh S.S., Ng S.S., de Oca M.M., De Labastida Rivera F., Chauhan S.B., Singh N., Faleiro R.J., Edwards C.L., Frame T.C., Sheel M., Austin R.J., Lane S.W., Bald T., Smyth M.J., Hill G.R., Best S.E., Haque A., Corvino D., Waddell N., Koufariotis L., Mukhopadhay P., Rai M., Chakravarty J., Singh O.P., Sacks D., Nylen S., Uzonna J., Sundar S, & Engwerda C.R. (2020). Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell reports, 30(8), 2512-2525.e9.
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