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K2hpo4

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K2HPO4 is a chemical compound that is commonly used in laboratory settings. It is a white, crystalline powder that is soluble in water. K2HPO4 is a chemical salt with the formula K2HPO4, which consists of two potassium ions and one hydrogen phosphate ion.

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Lab products found in correlation

Casamino acid, by BD (3 mentions) Glucose, by Carl Roth (2 mentions) Sodium chloride (nacl), by Carl Roth (2 mentions) Magnesium sulfate heptahydrate, by Merck Group (2 mentions) Kanamycin sulfate, by AppliChem (2 mentions) Yeast extract, by Thermo Fisher Scientific (2 mentions) Starch, by Merck Group (2 mentions) R2a agar, by Merck Group (2 mentions) Proteose peptone no 3, by BD (2 mentions) D glucose monohydrate, by Merck Group (2 mentions) Rifampicin, by Merck Group (2 mentions) Sodium pyruvate, by Merck Group (2 mentions) Na2hpo4, by Carl Roth (1 mentions) Thiamine, by Merck Group (1 mentions) Mgso4, by Carl Roth (1 mentions) Kh2po4, by Thermo Fisher Scientific (1 mentions) Nh4cl, by Merck Group (1 mentions)

4 protocols using k2hpo4

1

Comprehensive Media Preparation for Staphylococci and E. coli

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Basic medium (B medium) for Staphylococci consisted of Soy Peptone (10 g; Plato, Koblenz, Germany), Yeast Extract (5 g; Deutsche Hefewerke, Nuernberg, Germany), NaCl (5 g; Carl-Roth, Karlsruhe, Germany), Glucose (1 g; Carl Roth) and K2HPO4 (1 g; Applichem, Darmstadt, Germany). Deionized water was added to a final volume of 1 liter and pH was adjusted to 7.2.
LB medium for E. coli consisted of Peptone (10 g; Plato), Yeast Extract (5 g; Deutsche Hefewerke) and NaCl (5 g; Carl Roth). Deionized water was added to a final volume of 1 liter and pH was adjusted to 7.2. MacConkey agar was prepared according to the manufactures’ instructions (Carl Roth). Minimal media M9 for staphylococci consisted of Na2HPO4 (6 g; Carl Roth), KH2PO4 (3 g; Fisher Chemicals), NH4Cl (1 g; Merck Darmstadt, Germany) and NaCl (0.5 g; Carl Roth). Deionized water was added to a final volume of 1 L, the media was autoclaved and supplemented with thiamine (2 µg/mL; Sigma Aldrich, Munich, Germany), MgSO4 (1 mM; Carl Roth) and casamino acids (0.2%; BD Bioscience, Heidelberg, Germany). Cells were routinely grown at 37 °C either in liquid culture in baffled Erlenmeyer flasks (1:5 ratio) with shaking or on 1.5% agar plates.
Deibert J., Kühner D., Stahl M., Koeksoy E, & Bertsche U. (2016). The Peptidoglycan Pattern of Staphylococcus carnosus TM300—Detailed Analysis and Variations Due to Genetic and Metabolic Influences. Antibiotics, 5(4), 33.
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2

Microbial Growth Media Formulations

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The cells were grown in their respective medium to the needed OD. Basic medium (BM) for S. aureus consisted of Soy Peptone (10 g; Plato), Yeast Extract (5 g; Deutsche Hefewerke), NaCl (5 g; Carl-Roth), Glucose (1 g; Carl Roth) and K2HPO4 (1 g; Applichem). Deionized water was added to a final volume of 1 liter and pH was adjust to 7.2. LB medium for E. coli consisted of Peptone (10 g; Plato), Yeast Extract (5 g; Deutsche Hefewerke) and NaCl (5 g; Carl Roth). Deionized water was added to a final volume of 1 liter and pH was adjust to 7.2.
Kühner D., Stahl M., Demircioglu D.D, & Bertsche U. (2014). From cells to muropeptide structures in 24 h: Peptidoglycan mapping by UPLC-MS. Scientific Reports, 4, 7494.
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3

Cultivation Protocols for Diverse Bacterial Isolates

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Unless stated otherwise, At-SPHERE isolates were
cultivated at room temperature on R-2A agar (Sigma-Aldrich) or at 28°C in
R-2A broth (0.5 g yeast extract (Oxoid), 0.5 g Proteose peptone No. 3 (Becton,
Dickinson and Company), 0.5 g Casamino acids (Becton, Dickinson and Company),
0.5 g D-glucose monohydrate (Sigma-Aldrich), 0.5 g starch (from potato, Fluka),
0.3 g sodium pyruvate (Sigma-Aldrich), 0.3 g K2HPO4(AppliChem) and 0.05 g magnesium sulfate heptahydrate (Sigma-Aldrich) dissolved
in 1 L deionized water), both supplemented after sterilization with 0.5% (v/v)
methanol (R-2A+M). Minimal medium was prepared as described previously66 (link) and supplemented with 5 mM
maltose (Fluka). For Leaf374 cultivation, minimal medium agar was supplemented
with vitamins (500 μg L-1 D-pantothenic acid hemi calcium
salt, 100 μg L-1 biotin, 400 μg L-1riboflavin, 400 μg L-1 thiamine HCl, 200 μg
L-1 pyridoxal HCl 150 μg L-1 p-amino benzoic
acid, 200 μg L-1 cobalamin, 50 μg L-1 lipoic
acid, 150 μg L-1 nicotinic acid and 100 μg
L-1 folic acid). For selective growth of Leaf272, R-2A+M was
supplemented with 50 μg mL-1 rifampicin (Sigma-Aldrich), since
this strain has a natural resistance towards this antibiotic.
E. coli BL21 (DE3) gold was cultivated in LB-Lennox at
37°C supplemented with 50 μg mL-1 kanamycin sulfate
(AppliChem).
Schäfer M., Vogel C.M., Bortfeld-Miller M., Mittelviefhaus M, & Vorholt J.A. (2022). Mapping phyllosphere microbiota interactions in planta to establish genotype-phenotype relationships. Nature microbiology, 7(6), 856-867.
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4

Cultivation Protocols for Diverse Bacterial Isolates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless stated otherwise, At-SPHERE isolates were
cultivated at room temperature on R-2A agar (Sigma-Aldrich) or at 28°C in
R-2A broth (0.5 g yeast extract (Oxoid), 0.5 g Proteose peptone No. 3 (Becton,
Dickinson and Company), 0.5 g Casamino acids (Becton, Dickinson and Company),
0.5 g D-glucose monohydrate (Sigma-Aldrich), 0.5 g starch (from potato, Fluka),
0.3 g sodium pyruvate (Sigma-Aldrich), 0.3 g K2HPO4(AppliChem) and 0.05 g magnesium sulfate heptahydrate (Sigma-Aldrich) dissolved
in 1 L deionized water), both supplemented after sterilization with 0.5% (v/v)
methanol (R-2A+M). Minimal medium was prepared as described previously66 (link) and supplemented with 5 mM
maltose (Fluka). For Leaf374 cultivation, minimal medium agar was supplemented
with vitamins (500 μg L-1 D-pantothenic acid hemi calcium
salt, 100 μg L-1 biotin, 400 μg L-1riboflavin, 400 μg L-1 thiamine HCl, 200 μg
L-1 pyridoxal HCl 150 μg L-1 p-amino benzoic
acid, 200 μg L-1 cobalamin, 50 μg L-1 lipoic
acid, 150 μg L-1 nicotinic acid and 100 μg
L-1 folic acid). For selective growth of Leaf272, R-2A+M was
supplemented with 50 μg mL-1 rifampicin (Sigma-Aldrich), since
this strain has a natural resistance towards this antibiotic.
E. coli BL21 (DE3) gold was cultivated in LB-Lennox at
37°C supplemented with 50 μg mL-1 kanamycin sulfate
(AppliChem).
Schäfer M., Vogel C.M., Bortfeld-Miller M., Mittelviefhaus M, & Vorholt J.A. (2022). Mapping phyllosphere microbiota interactions in planta to establish genotype-phenotype relationships. Nature microbiology, 7(6), 856-867.
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