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The ITD-1 is a precision instrument designed for isothermal titration calorimetry (ITC) analysis. It measures the heat released or absorbed during a chemical or biological interaction, providing insights into the thermodynamics of the process.

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2 protocols using itd 1

1

TGF-β Signaling Pathway Inhibitor Assay

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ITD-1, a TGF-β signaling pathway inhibitor, was purchased from Macklin (1884570, Shanghai, China). Abcam provided the anti-GAL-1 antibody (ab138513, Cambridge, UK); Cell Signaling Technology provided the anti-SMA-α antibody (56856, Danvers, MA, USA); anti-E-cadherin (bs-10009R), anti-Vimentin (bs-0756R), anti-TGF-β1 (bs-0086R), and anti-p-Smad2/3(bs-8853R) antibodies were purchased from Bioss (Beijing, China); Santa Cruz Biotechnology (Santa Cruz, CA, USA) provided the anti-glyceraldehyde phosphate dehydrogenase (GAPDH) antibody (sc-47724), the goat anti-rabbit IgG (sc-2357), the horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-516102); Sigma Biotechnology (St. Louis, MO, USA) provided the MTT assay kit and dimethyl sulfoxide (DMSO).
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2

Hedgehog Signaling Pathway Inhibitor ITD-1 Protocol

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ITD-1, a hedgehog signaling pathway inhibitor, was purchased from Macklin (1884570, Shanghai, China). The anti-GAL-1 antibody was purchased from Abcam (ab138513, Cambridge, UK), the anti-SMA-α antibody was purchased from Cell Signaling Technology (56856, Danvers, MA, USA), anti-E-cadherin (bs-10009R), anti-Vimentin (bs-0756R), anti-TGF-β1 (bs-0086R), and anti-p-Smad2/3(bs-8853R) antibodies were purchased from Bioss (Beijing, China), and the anti-glyceraldehyde phosphate dehydrogenase (GAPDH) antibody (sc-47724), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (sc-516102), and goat anti-rabbit IgG (sc-2357) antibodies were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). The dimethyl sulfoxide (DMSO) and MTT assay kit and were provided by Sigma Biotechnology (St. Louis, MO, USA).
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