MOLM-13 (ATCC, Manassas, VA) cells were maintained in RPMI-1640 (Fisher Scientific, Waltham, MA) with 10% fetal bovine serum (Fisher Scientific, Waltham, MA). DS-3032b (milademetan) or DS-5272 (provided by Daiichi-Sankyo, Chuo, Japan) was dissolved in DMSO and added to cells one day before AZA (Sigma-Aldrich, St. Louis, MO) treatment, which lasted for three days. The coefficient of drug interaction (CDI) is calculated as follows: CDI = AB/(A×B). AB is the ratio of the combination groups to control group; A or B is the ratio of the single agent group to control group. Thus, CDI value <1 indicates that the drugs are synergistic [20 (link)].
Ds 5272
Manufactured by Daiichi Sankyo
Sourced in Japan
The DS-5272 is a high-performance liquid chromatography (HPLC) system designed for laboratory use. It is capable of separating and analyzing a wide range of chemical compounds with high precision and accuracy.
Automatically generated - may contain errors
Lab products found in correlation
Ds 3032b, by Daiichi Sankyo (2 mentions)
B6 cg tg, by Jackson ImmunoResearch (2 mentions)
B6 cg commd10tg vav1 icre a2kio j, by Jackson ImmunoResearch (2 mentions)
Mdm2tm2.1glo j, by Jackson ImmunoResearch (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Molm 13, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Kaluza flow cytometry analysis software, by Beckman Coulter (1 mentions)
Ovtoko, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Caki 1, by American Type Culture Collection (1 mentions)
Ovise, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Caki 2, by American Type Culture Collection (1 mentions)
Jhoc 7, by RIKEN Cell Bank (1 mentions)
Jhoc 9, by RIKEN Cell Bank (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Everolimus, by LC Laboratories (1 mentions)
B6.129 cg gt rosa 26sortm4 actb tdtomato, by Jackson ImmunoResearch (1 mentions)
B6.129p2 trp53tm1brn j, by Jackson ImmunoResearch (1 mentions)
5 protocols using ds 5272
1
Synergistic Evaluation of DS-3032b and Azacitidine in MOLM-13 Cells
2
Mouse Model of Tet2 Deficiency
3
Comparative Analysis of CCC Cell Lines
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We used 6 human CCOC cell lines (OVISE, OVTOKO, JHOC‐7, JHOC‐9, ES‐2, and SKOV‐3), 2 CCRC cell lines (Caki‐1 and Caki‐2), and 1 mouse ovarian carcinoma cell line (ID8). The OVISE and OVTOKO cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank. JHOC‐7 and JHOC‐9 cell lines were purchased from the RIKEN Cell Bank. ES‐2, SKOV3, Caki‐1, and Caki‐2 cell lines were purchased from ATCC. ID8 cells were kindly gifted by Dr Kathy Roby of the Department of Anatomy and Cell Biology at the University of Kansas Medical Center. All cell lines were cultured at 37°C in a humidified incubator with 5% CO2. All 9 cell lines were classified histologically as CCCs and authenticated by short tandem repeat analysis. We used the International Cell Line Authentication Committee database to confirm that these cell lines were not cross‐contaminated or misidentified. The MDM2 inhibitors DS‐3032b and DS‐5272 were provided by the Daiichi‐Sankyo Company, and the mTOR inhibitor everolimus was purchased from LC Laboratories.
4
Genetic Mice Models for MDM2 Inhibitor Treatment
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Mdm2-floxed mice (Mdm2tm2.1Glo/J), mTmG mice (B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J), Trp53-floxed mice (B6.129P2-Trp53tm1Brn/J ), Osx-Cre mice (B6.Cg-Tg(Sp7-tTA,tetO-EGFP/Cre)1Amc/J), and Vav-Cre mice (B6.CgCommd10Tg(Vav1-icre)A2Kio/J ) were purchased from The Jackson Laboratories. Animals were housed in the MD Anderson Cancer Center animal facility, and all procedures using animals were approved by the Institutional Animal Care and Use Committee. For MDM2i treatment, mice were treated by oral gavage with DS5272 (Daiichi Sankyo, 100 mg/kg, three times/week) for two weeks and euthanized by asphyxiation with CO2 for analysis 24 hours after the last dose.
5
Genetically Modified Mouse Models for MDM2 Inhibitor Study
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Mdm2-floxed mice (Mdm2tm2.1Glo/J), mTmG mice (B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J), Trp53-floxed mice (B6.129P2-Trp53tm1Brn/J), Osx-Cre mice (B6.Cg-Tg(Sp7-tTA,tetO-EGFP/Cre)1Amc/J), and Vav-Cre mice (B6.Cg-Commd10Tg(Vav1-icre)A2Kio/J) were purchased from The Jackson Laboratories. Experiments were conducted on age-matched mice between 8 and 12 weeks of age to ensure consistency and reduce variability in the results. Animals were housed in the MD Anderson Cancer Center animal facility, and all procedures using animals were approved by the Institutional Animal Care and Use Committee. For MDM2i treatment, mice were treated by oral gavage with DS5272 (Daiichi Sankyo, 100 mg/kg, three times/week) for two weeks and euthanized by asphyxiation with CO2 for analysis 24 h after the last dose.
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