The standard sugars, namely, glucose, fructose, trehalose, and maltose monohydrate, were procured from Sigma-Aldrich (USA). Trehalulose was purchased from Carbosynth Ltd. (UK). Luria-Bertani broth, Luria-Bertani agar, yeast extract, peptone, malt extract, Coomassie blue, imidazole, Tris base, and sucrose were purchased from HiMedia (India). The restriction enzymes and molecular biology kits were procured from New England BioLabs (NEB; USA) and Thermo-Fischer Scientific (USA). The pJET1.2 cloning vector and DNA and protein ladders were obtained from Thermo-Fisher Scientific. The expression vector (pET28a) and host Escherichia coli BL21(DE3) were procured from Novagen. Ammonium persulphate, sodium acetate, sodium chloride, and isopropyl β-d -1-thiogalactopyranoside (IPTG) were purchased from Sigma-Aldrich. The nickel-chelating nitrilotriacetic acid (Ni-NTA) agarose gel was procured from Qiagen (Germany). Unrefined and partially refined sugars like muscovado, jaggery, and table sugar were procured from the local market. Sugarcane molasses was purchased from Saraswati Sugar Mills Ltd., Yamuna Nagar, Haryana, India. All the solvents for TLC and HPLC analyses were procured from CDH (India).
Imidazole
Manufactured by HiMedia
Sourced in India
Imidazole is a heterocyclic organic compound with the chemical formula C3H4N2. It is a colorless crystalline solid that is soluble in water and organic solvents. Imidazole serves as a core functional group in various biomolecules and is commonly used in the synthesis of pharmaceuticals and other chemical compounds.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (4 mentions)
Luria bertani broth, by HiMedia (3 mentions)
Glycerol, by Merck Group (3 mentions)
Lysozyme, by HiMedia (3 mentions)
Methanol, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Potassium hydroxide, by Merck Group (2 mentions)
Hepes, by Merck Group (2 mentions)
Urea, by Avantor (2 mentions)
Ammonium sulfate, by HiMedia (2 mentions)
Q sepharose, by GE Healthcare (2 mentions)
Potassium chloride (kcl), by HiMedia (2 mentions)
Sodium dodecyl sulfate (sds), by HiMedia (2 mentions)
β mercaptoethanol, by Merck Group (2 mentions)
Ethylene diamine tetraacetic acid (edta), by HiMedia (2 mentions)
Sodium acetate, by Merck Group (1 mentions)
Fructose, by Merck Group (1 mentions)
Trehalose, by Merck Group (1 mentions)
Ammonium persulphate, by Merck Group (1 mentions)
Maltose monohydrate, by Merck Group (1 mentions)
6 protocols using imidazole
1
Enzymatic Synthesis of Trehalulose
2
Oligomer and Fibril Amyloid Assay
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Sodium phosphate dibasic dihydrate, magnesium chloride hexahydrate, ThT, adenosine-5′-triphosphate disodium salt hydrate (ATP), dimethyl sulfoxide, guanidinium hydrochloride, β-mercaptoethanol, and Hepes were procured from Sigma. Urea and PK were bought from Amresco. SDS, imidazole, ammonium sulfate, lysozyme, potassium chloride, and EDTA were purchased from HIMEDIA. Sodium hydroxide, potassium hydroxide, glycerol, methanol, nitrocellulose membrane, A11 (anti–amyloid oligomer antibody), OC (anti–amyloid fibril antibody), horseradish peroxidase–conjugated goat anti-rabbit antibody, and sodium chloride were bought from Merck. Antibiotics (ampicillin and chloramphenicol) and IPTG were procured from Gold Biocom. Q-sepharose and nickel–nitrilotriacetic acid columns were bought from GE Healthcare Lifesciences. Fluorescein-5-maleimide was procured from Invitrogen. About 96-well NUNC optical bottom plates were purchased from ThermoFisher Scientific.
3
Purification and Characterization of Amyloid Aggregates
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Hepes, magnesium chloride hexahydrate, sodium phosphate dibasic dihydrate, tris (hydroxymethyl) aminomethane (Tris), β-mercaptoethanol, ATP disodium salt hydrate, DTT, and ThT were bought from Sigma. GdmCl, proteinase K, and urea were procured from Amresco. Ammonium sulfate, imidazole, lysozyme, SDS, EDTA, and potassium chloride was bought from HIMEDIA. Potassium hydroxide, sodium chloride, sodium hydroxide, glycerol, A-11 antiamyloid oligomer antibody, methanol, horseradish peroxide (HRP)–conjugated goat anti-rabbit antibody was procured from Merck. IPTG and antibiotics (chloramphenicol and ampicillin) were purchased from Gold Biocom. Enhanced chemiluminescence kit, HRP-conjugated rabbit antimouse antibody, was obtained from Thermo Fisher Scientific. Nickel-nitriloacetic acid (Ni-NTA) column and Q-Sepharose were from GE Healthcare Lifesciences. Phosphoenolpyruvate (PEP) and pyruvate kinase (PK) were procured from Roche Diagnostics.
4
Recombinant zDHFR Protein Expression and Purification
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Recombinant zDHFR protein were expressed and purified by BL21-DE3 Rosetta E. coli cells. Plasmid pET43.1a, which was carrying zDHFR gene obtained from Dr. Tzu-Fun, Taiwan. Isopropyl-d -1-thiogalactopyranoside (IPTG), Dihydrofolic acid (DHF), Nicotinamide Adenine Di-nucleotide Phosphate (NADPH), Sodium citrate (Na3C6H5O7), Tannic acid (C76H52O46), Silver Nitrate (AgNO3), Oxidized (GSSG) and reduced (GSH) form of glutathione, high purity grade Imidazole, Magnesium chloride (MgCl2), Lysozyme, Phenylmethylsulfonyl fluoride (PMSF), Sodium phosphate buffer, pH 7.4 (Sodium dihydrogen Phosphate (NaH2PO4), Disodium Phosphate (Na2HPO4)), Tris Hydrochloride (Tris HCl), Potassium chloride (KCl), 5, 5-dithiobis (2-nitrobenzoic acid) (DTNB), Sodium chloride (NaCl), Dialysis membrane and Coomassie Blue R-250 were purchased from HiMedia, India. The buffer for unfolding experiments contains 20 mM Tris, 25 mM KCl, 1 mM reduced glutathione (GSH), 0.1 mM oxidized glutathione (GSSG), 10% Glycerol, pH 7.4. Refolding of zDHFR was performed in 20 mM Tris, 25 mM KCl, 10% Glycerol, 1 mM oxidized glutathione (GSSG), pH 7.4. Tris KCl buffer (20 mM Tris, 25 mM KCl, pH 7.4). All other reagents were of analytical grade. Double distilled water or Milli-Q (Merck Millipore) was used for experimentation.
5
Recombinant Tyrosine Protein Expression in E. coli
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The expression host Escherichia coli (E. coli) tyrosine auxotroph was received from Coli Genetic Stock Center, and the pQE80-L vector was purchased from Qiagen (Valencia, USA). T4 DNA ligase and restriction enzymes were purchased from New England Biolabs (Ipswich, US). Natural amino acids, M9 salts, 3,4-dihydroxy-L-phenylalanine (L-Dopa), ferrocene carboxaldehyde 98%, and chitosan (medium molecular weight) were purchased from Sigma-Aldrich (Bangalore, India). Isopropyl β-d-1-thiogalactopyranoside (IPTG), ampicillin, Luria–Bertani (LB) broth, and imidazole were purchased from HiMedia (Mumbai, India). His trap HP protein purification column was purchased from GE Healthcare (Bangalore, India). Used as a working buffer solution was 0.01 M phosphate buffer saline pH 7.4. chitosan solutions (2%, pH 5.5) were prepared by dissolving chitosan in 0.25 N HCl.
6
Purification of Recombinant Proteins
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Sodium chloride, imidazole, GdmCl, urea, glycine, ethanol, Tris buffer, etc. were brought from Merck (Darmstadt, Germany). Glycerol, N-lauroylsarcosine, and Triton X-100 were purchased from Sigma (St. Louis, MI, USA). Luria–Bertani broth, kanamycin, and imidazole were brought from Himedia, India. All the chemicals used for the experiments were of analytical grade.
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