Forty-five primary canines lacking preexisting cuspal wear, caries, and fractures extracted for orthodontic reasons were included in the study out of 67 collected teeth after applying exclusion criteria. The extracted specimens were collected, stored in normal saline, sterilized, and handled according to the Occupational Safety and Health Administration (OSHA) guidelines and regulations. The primary canine specimens were prepared by mounting them in an acrylic resin (orthodontic resin, Dentsply, Philadelphia, PA, USA), measuring 20 mm × 20 mm × 10 mm, and approximately 5 mm cusps of the primary canine teeth were exposed (
Orthodontic resin
Orthodontic resin is a laboratory product used in the fabrication of dental appliances. It is a material designed for use in the construction of orthodontic devices, such as retainers and aligners, to support and correct the alignment of teeth.
12 protocols using orthodontic resin
Evaluating Dental Restorative Materials
Forty-five primary canines lacking preexisting cuspal wear, caries, and fractures extracted for orthodontic reasons were included in the study out of 67 collected teeth after applying exclusion criteria. The extracted specimens were collected, stored in normal saline, sterilized, and handled according to the Occupational Safety and Health Administration (OSHA) guidelines and regulations. The primary canine specimens were prepared by mounting them in an acrylic resin (orthodontic resin, Dentsply, Philadelphia, PA, USA), measuring 20 mm × 20 mm × 10 mm, and approximately 5 mm cusps of the primary canine teeth were exposed (
Horizontal Sectioning of Root Specimens
Each specimen was sectioned horizontally using a diamond disk (Buehler, Lake Bluff, IL, USA) with a slow-speed handpiece (25,000 rpm) at 2 and 4 mm from the apex. The cut samples were then mounted onto glass slides, and the coronal surface underwent a polishing phase using sandpapers of 500, 700, and 1200 grit under running water to eliminate the dentin debris that could be produced during root canal shaping produce a clear reflective surface. The samples examined with confocal laser microscopy were 2 mm thick.
Embedding and Sectioning Dental Specimens
Implant Fixture Mounting Protocol
Surface Energy and Shear Bond Strength of Y-TZP
Evaluating Ceramic Coping Fit using CSM
Maxillary Premolar Extraction Protocol
Fabricating Teeth Mounting Mold
Quantifying Root Canal Sealer Penetration
Each specimen was sectioned horizontally utilizing a diamond disk (Buehler, Lake Bluff, IL, USA) attached to a low-speed handpiece (25,000 rpm) at 2 and 4 mm from the apex. The cut specimens were then mounted onto glass slides, and the coronal surface underwent a polishing phase using sandpapers of 500, 700, and 1200 grit under running water to eradicate the dentin debris that could be created during root preparation to produce a clear reflective surface. The samples examined with confocal laser microscopy were 2 mm thick, and analysis of the roots using confocal laser scanning microscopic imaging.
Root canal segments were studied with a Zeiss confocal laser scanning microscope (CLSM) (Carl Zeiss, LSM 780, Jena, Germany) at 10 magnifications and set in fluorescent mode (at a wavelength of 514 nm) in
The measurements were made by operators blinded to which samples corresponded to which Group (A, B, or C). The measurements were repeated twice to ensure reproducibility.
Primary Canine Tooth Preparation for Research
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