M3814 was purchased from Medchemexpress (MCE, HY-101570). Paclitaxel and etoposide phosphate were purchased form Selleck Chemicals (S1150) and Meilunbio (MB1102), respectively. For in vitro studies, M3814 and etoposide was initially dissolved in dimethyl sulfoxide (DMSO; Sigma) as 100 mmol/L stock solution and stored at −20 °C. The stock solution was diluted to reach corresponding concentrations and added to cell cultures. Treatment controls were set as the 0.5% DMSO, the same volume as the other groups. For in vivo experiments, M3814 were orderly added with the following solvent: 10% DMSO, 40% PEG400 (Sigma–Aldrich), 5% Tween-80 (Sigma–Aldrich) and 45% saline. Anti-phospho-histone γ-H2AX (Ser139) was purchased from Abcam (ab11174; Cambridge, UK). Anti-P53 antibody was purchased from HUABIO (ET1601-13; Hangzhou, China). Anti-P21 (2947S), anti-DNA-PKcs (12311S) and anti-p-DNA-PK (Ser2056, 68716S) antibodies were purchased from Cell Signaling Technology (CST, MA, USA). Anti-GAPDH antibody was purchased from Santa Cruz Biotechnology (sc-32233; Santa Cruz, CA, USA). Fluorescent secondary antibodies were obtained from Invitrogen.
M3814
Manufactured by Merck Group
Sourced in Germany
The M3814 is a lab equipment product from Merck Group. It is a versatile piece of equipment designed for use in research and laboratory settings. The core function of the M3814 is to provide accurate and reliable measurements for various applications. Detailed specifications and intended use are not available for this product.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Peg400, by Merck Group (1 mentions)
Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Anti p21 2947, by Cell Signaling Technology (1 mentions)
Tween 80, by Merck Group (1 mentions)
Fluorescent secondary antibody, by Thermo Fisher Scientific (1 mentions)
Paclitaxel, by Selleck Chemicals (1 mentions)
M3814, by MedChemExpress (1 mentions)
Ibrutinib, by Selleck Chemicals (1 mentions)
Dasatinib, by Cayman Chemical (1 mentions)
Nu7441, by Selleck Chemicals (1 mentions)
Fty720, by Cayman Chemical (1 mentions)
Z ietd fmk, by Selleck Chemicals (1 mentions)
Daunorubicin, by Selleck Chemicals (1 mentions)
Idarubicin, by Selleck Chemicals (1 mentions)
Sunitinib, by Selleck Chemicals (1 mentions)
Etoposide, by Selleck Chemicals (1 mentions)
5 protocols using m3814
1
M3814 Pharmacological Evaluation Protocol
2
Evaluating the Efficacy of DNA-PK and KIT Inhibitors
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Cell lines were treated with the following agents, either alone or in combination as indicated in text. DNA-PK inhibitors: NU7441 (Selleckchem), M3814 (Merck); KIT signaling inhibitors: dasatinib (Cayman Chemical), ibrutinib (Selleckchem), FTY720 (Cayman Chemical), and AAL(S) (synthesized by A/Prof Jonathan Morris, School of Chemistry, UNSW as described (43 (link))). Dimethyl sulfoxide was used as the solvent for all compounds. Final vehicle concentration was below 0.1% for all experiments.
3
Anti-mouse PD-L1 mAb Protocol
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InVivoMAb anti-mouse PD-L1 (B7-H1) was purchased from BioCell (catalog# BE0101). M3814 was obtained from Merck (Darmstadt, Germany).
4
Evaluation of Apoptosis Inducers
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The DNA-PK inhibitor, M3814, was synthesized at Merck KGaA, Darmstadt, Germany as described. CPX-351 (Vyxeos®) was provided by Jazz Pharmaceuticals as part of a grant from CTEP. Daunorubicin, etoposide, idarubicin, sunitinib, and Z-IETD-FMK were purchased from Selleckchem (Houston, TX). Arabinoside (Cytarabine, AraC) and TRAIL were purchased from MilliporeSigma (Burlington, MA). All inhibitors were dissolved in DMSO to prepare a 10 mM stock and stored at − 20 °C. Aliquots were diluted directly in tissue culture media containing 10% FCS to a desired concentration. The final concentration of DMSO in media did not exceed 0.11% (vol/vol) which has not shown detectable effect on cell morphology, viability and differentiation potential.
5
Small Molecule Effects on iPSC Editing
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Commercially available small molecules used in this study were trichostatin A (TSA) (Cayman; 89,730) and M3814 (MedKoo; 206,478). iPSCs were split into two culture wells after electroporation with editing plasmids to assess the effects of small molecules. Stock solutions of the HDAC inhibitor TSA and NHEJ inhibitor M3814 were prepared in dimethylsulfoxide (DMSO) (Sigma) and diluted to working concentrations before use. The parallel well with only DMSO (0.1%) served as a control. The medium was changed 24 h after the addition of small molecules.
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