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Iv100

Manufactured by Olympus
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Sourced in Japan

The IV100 is a stereo microscope designed for in vivo imaging. It features high-performance optics and advanced imaging capabilities to enable detailed observation and analysis of biological samples.

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Lab products found in correlation

Fv1000, by Olympus (3 mentions) Vs120 fl, by Olympus (2 mentions) Ov100, by Olympus (2 mentions) Ov100 small animal imaging system, by Olympus (2 mentions) Osteosense 750ex, by PerkinElmer (2 mentions) Iv10 asw, by Olympus (2 mentions) Osteosense 750ex nev10053ex, by PerkinElmer (2 mentions) X cite exacte, by Excelitas (1 mentions) Orca r2, by Hamamatsu Photonics (1 mentions) Cd31 alexa fluor 647, by BioLegend (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Sp dioc18 3, by Thermo Fisher Scientific (1 mentions) Celltracker cm dil, by Thermo Fisher Scientific (1 mentions) Rl 1102, by Vector Laboratories (1 mentions)

Spelling variants of this product

IV100 confocal microscope (3 citations) IV100 laser scanning microscope (2 citations) IV100 microscope (2 citations)

10 protocols using iv100

1

Cellular-Resolution Imaging of Tumors

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Images were obtained on Olympus FV1000 and IV100 confocal microscopes. Wide-field images were obtained by stitching a 5 × 4 grid of image stacks acquired with a × 2 NA 0.95 objective and presented as maximum intensity projections. A × 10 NA 0.6 water objective provided cellular resolution images,1.44 mm2x–y coverage, and was suitable for two-photon imaging. × 20 NA 0.95 or × 40 NA 1.15 water objectives were also used when higher resolution was desired. All images were acquired in sequential mode and acquisition of stacks was started at maximum depth to offset bleaching effects. A 1 mm3 tumour could be imaged at cellular resolution (× 10 objective, 8 μs px−1 512 × 512 px) in ∼10 min. Wide-field grids were acquired from both sides of the lungs in about 13 min per side. Grids were assembled in Image J, when necessary, mediastinum was digitally removed for presentation (Supplementary Fig. 5), and sides were co-registered for 3D viewing in FEI Amira (Supplementary Movie 3).
Cuccarese M.F., Dubach J.M., Pfirschke C., Engblom C., Garris C., Miller M.A., Pittet M.J, & Weissleder R. (2017). Heterogeneity of macrophage infiltration and therapeutic response in lung carcinoma revealed by 3D organ imaging. Nature Communications, 8, 14293.
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2

Fluorescence Imaging System Specifications

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The basic specifications of fluorescence imaging systems has been reported previously.24 Briefly, macroscopic images were captured using a fluorescence reflectance imaging system (OV100; Olympus, Tokyo, Japan) equipped with a 150 W xenon light source and a color digital CCD camera (DP71; Olympus). Excitation and emission filters for ICG fluorescence imaging were 730 ± 22.5‐nm band‐pass and 770‐nm long‐pass filter, respectively. High‐power view was observed using a multi‐wavelength laser scanning microscope (IV100; Olympus) equipped with a 748‐nm diode laser with 17 mW power output and a photomultiplier tube. The emission filter to detect ICG fluorescence was a 779‐nm long‐pass filter. Tissue sections were scanned using a fluorescence virtual microscopy system (VS120‐FL; Olympus) equipped with a 200 W mercury light source (X‐Cite exacte; Excelitas Technologies, Waltham, MA, USA) and a monochrome digital CCD camera (ORCA‐R2; Hamamatsu Photonics, Hamamatsu, Japan). Excitation and emission filters for ICG fluorescence were 708 ± 37.5‐nm band‐pass and 809 ± 40.5‐nm band‐pass filter, respectively. Fluorescence images are shown in grayscale or pseudo‐colors.
Nagahara R., Onda N., Yamashita S., Kojima M., Inohana M., Eguchi A., Nakamura M., Matsumoto S., Yoshida T, & Shibutani M. (2018). Fluorescence tumor imaging by i.v. administered indocyanine green in a mouse model of colitis‐associated colon cancer. Cancer Science, 109(5), 1638-1647.
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3

In Vivo Imaging of PDOX Tumors

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PDOX tumors, labeled with genetic reporters, were imaged with a laser-scanning microscope (IV100; Olympus, Tokyo, Japan) 27 (link) or a confocal laser-scanning microscope (FV1000; Olympus) 28 (link) or an OV-100 Olympus Small Animal Imaging System (Olympus Corp.).29 (link)
Yano S., Hiroshima Y., Maawy A., Kishimoto H., Suetsugu A., Miwa S., Toneri M., Yamamoto M., Katz M.H., Fleming J.B., Urata Y., Tazawa H., Kagawa S., Bouvet M., Fujiwara T, & Hoffman R.M. (2015). Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery. Cancer gene therapy, 22(7), 344-350.
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4

Multimodal Imaging for Cell and Tissue Analysis

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Microscopic imaging was performed using a fluorescence reflectance imaging system (OV100; Olympus Corporation, Tokyo, Japan) and a multi-wavelength laser scanning microscope (IV100; Olympus) to acquire wide-field and high-power images, respectively [18 (link)]. Live cells and tissue sections were scanned using a fluorescence virtual microscopy system (VS120-FL; Olympus) [18 (link)]. Endoscopic imaging was performed using an in-house endoscopic system, as described previously [14 (link)]. ICG fluorescence was excited by a xenon light source through a 600 ± 200-nm band-pass filter and detected by a sensitive electron-multiplying charge-coupled device camera (MC285SPD-L0B0; Texas Instruments, Dallas, TX, USA) through a 842.5 ± 17.5-nm band-pass filter. As a flexible endoscope, a bronchoscope fiberscope (BF-XP60; Olympus Medical Corporation, Tokyo, Japan), 2.8 mm in diameter with a single biopsy channel, was used. Fluorescence images were shown in grayscale or pseudo-colors.
Onda N., Mizutani-Morita R., Yamashita S., Nagahara R., Matsumoto S., Yoshida T, & Shibutani M. (2017). Fluorescence contrast-enhanced proliferative lesion imaging by enema administration of indocyanine green in a rat model of colon carcinogenesis. Oncotarget, 8(52), 90278-90290.
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5

In Vivo Imaging of PDOX Tumors

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PDOX tumors, labeled with genetic reporters, were imaged with a laser-scanning microscope (IV100; Olympus, Tokyo, Japan) 27 (link) or a confocal laser-scanning microscope (FV1000; Olympus) 28 (link) or an OV-100 Olympus Small Animal Imaging System (Olympus Corp.).29 (link)
Yano S., Hiroshima Y., Maawy A., Kishimoto H., Suetsugu A., Miwa S., Toneri M., Yamamoto M., Katz M.H., Fleming J.B., Urata Y., Tazawa H., Kagawa S., Bouvet M., Fujiwara T, & Hoffman R.M. (2015). Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery. Cancer gene therapy, 22(7), 344-350.
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6

Microscopic Imaging of Pancreatic Tumors

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The IV-100 (Olympus) and the conjugated antibody were used to obtain microscopic fluorescence images of pancreatic tumors and the normal pancreas in the orthotopic mouse model. The IV-100 operates with four lasers (488, 561, 633, and 748 nm) for excitation; three of which can be used simultaneously for imaging. Its microprobe lens has an external diameter of 1.3 mm and delivers high resolution images in the visible and near-infrared spectrum. Due to its small size, it can be used to image abdominal organs through a small incision [42 (link)].
Park J.Y., Lee J.Y., Zhang Y., Hoffman R.M, & Bouvet M. (2016). Targeting the insulin growth factor-1 receptor with fluorescent antibodies enables high resolution imaging of human pancreatic cancer in orthotopic mouse models. Oncotarget, 7(14), 18262-18268.
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7

Intravital Microscopy of Bone Marrow B Cells

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For serial intravital microscopy of the calvarium, bone marrow B cell precursors (Lineage− CD93+) from dsRed reporter mice and mature B cells from the spleen (CD19+ IgD+) were FACS-sorted using a FACSAria II cell sorter (BD). For B cell progenitor imaging experiments, 106 cells were injected i.v. into non-irradiated recipient C57BL/6 mice one day prior to stroke induction. To visualize mature B cell accumulation in the bone marrow, 5×106 cells were injected i.v. one week prior to surgeries. To highlight bone architecture, OsteoSense® 750EX, a fluorescent bisphosphonate imaging agent (Perkin Elmer), was administered i.v. 24 hours prior to imaging (4 nmol/mouse, PerkinElmer). To outline the vasculature, 15μg CD31-Alexa Fluor 647 (102516, BioLegend) 30 min prior to imaging. In vivo imaging was performed using a confocal microscope (IV100 Olympus). Z-stack images for each location were acquired at 2μm steps, and post-processing was performed using Image J software (NIH).
Courties G., Frodermann V., Honold L., Zheng Y., Herisson F., Schloss M.J., Sun Y., Presumey J., Severe N., Engblom C., Hulsmans M., Cremer S., Rohde D., Pittet M.J., Scadden D.T., Swirski F.K., Kim D.E., Moskowitz M.A, & Nahrendorf M. (2019). Glucocorticoids Regulate Bone Marrow B Lymphopoiesis After Stroke. Circulation research, 124(9), 1372-1385.
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8

Intravital Imaging of Calvarium

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Intravital microscopy of the calvarium was performed in ChatGFP mice using a confocal microscope (IV100 Olympus) and IV10-ASW 01.01.00.05 software (Olympus, Tokyo, Japan). OsteoSense 750EX (NEV10053EX, 4 nmol per mouse, PerkinElmer) was administered to outline bone architecture, CD31-PE (102508, clone MEC13.3, 15 μg per mouse, BioLegend) and Sca1-PE (12–5981-83, clone D7, 15 μg per mouse, eBioscience) was injected i.v. to outline vasculature and B220-APC (103212, clone RA3–6B2, 15 μg per mouse, BioLegend) to identify B cells.
Schloss M.J., Hulsmans M., Rohde D., Lee I.H., Severe N., Foy B.H., Pulous F.E., Zhang S., Kokkaliaris K.D., Frodermann V., Courties G., Yang C., Iwamoto Y., Knudsen A.S., McAlpine C.S., Yamazoe M., Schmidt S.P., Wojtkiewicz G.R., Masson G.S., Gustafsson K., Capen D., Brown D., Higgins J.M., Scadden D.T., Libby P., Swirski F.K., Naxerova K, & Nahrendorf M. (2022). B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis. Nature immunology, 23(4), 605-618.
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9

Intravital Imaging of Calvarium

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Intravital microscopy of the calvarium was performed in ChatGFP mice using a confocal microscope (IV100 Olympus) and IV10-ASW 01.01.00.05 software (Olympus, Tokyo, Japan). OsteoSense 750EX (NEV10053EX, 4 nmol per mouse, PerkinElmer) was administered to outline bone architecture, CD31-PE (102508, clone MEC13.3, 15 μg per mouse, BioLegend) and Sca1-PE (12–5981-83, clone D7, 15 μg per mouse, eBioscience) was injected i.v. to outline vasculature and B220-APC (103212, clone RA3–6B2, 15 μg per mouse, BioLegend) to identify B cells.
Schloss M.J., Hulsmans M., Rohde D., Lee I.H., Severe N., Foy B.H., Pulous F.E., Zhang S., Kokkaliaris K.D., Frodermann V., Courties G., Yang C., Iwamoto Y., Knudsen A.S., McAlpine C.S., Yamazoe M., Schmidt S.P., Wojtkiewicz G.R., Masson G.S., Gustafsson K., Capen D., Brown D., Higgins J.M., Scadden D.T., Libby P., Swirski F.K., Naxerova K, & Nahrendorf M. (2022). B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis. Nature immunology, 23(4), 605-618.
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10

Intravital Microscopy of Bone Stem Cells

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For serial intravital microscopy of the calvarium, SLAM HSCs were FACS-sorted, labeled with DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate, Molecular Probes) and injected i.v. into non-irradiated Nestin-GFP recipient mice. The GFP signal, while not specific for mesenchymal stem cells, guided revisiting similar regions of interest in serial intravital imaging. To highlight bone architecture, OsteoSense 750EX was administered (PerkinElmer). We used rhodamine-labelled Griffonia simplicifolia lectin (RL-1102, Vector Laboratories) to outline the vasculature. In vivo imaging was performed on days 1 and 5 after the adoptive cell transfer with a confocal microscope (IV100 Olympus). Z-stacks images for each location were acquired at 2μm steps. For visualization of LKS cells in sternum, Lin− c-Kit+ Sca-1+ cells were FACS sorted and labeled ex-vivo with two different fluorescent dyes including CellTracker™ CM-Dil and SP-DiOC18(3) (Molecular Probes) prior to adoptive transfer into recipient mice.
Courties G., Herisson F., Sager H.B., Heidt T., Ye Y., Wei Y., Sun Y., Severe N., Dutta P., Scharff J., Scadden D.T., Weissleder R., Swirski F.K., Moskowitz M.A, & Nahrendorf M. (2014). Ischemic Stroke Activates Hematopoietic Bone Marrow Stem Cells. Circulation research, 116(3), 407-417.
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