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Assay kits for glutathione

Manufactured by Cayman Chemical
Sourced in United States

Assay kits for glutathione are laboratory products designed to quantify the level of glutathione, a crucial antioxidant, in biological samples. These kits provide the necessary reagents and protocols to perform colorimetric or fluorometric assays to measure the concentration of reduced (GSH) and oxidized (GSSG) forms of glutathione.

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2 protocols using assay kits for glutathione

1

Dioscorea batatas Root Antioxidant Assessment

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The root of Dioscorea batatas Decne was obtained from Andong Bukhu Nonghyup (Gyeongbuk, Korea), freeze-dried and powdered. AOM was purchased from Sigma (St. Louis, MO, USA). The assay kits for glutathione (GSH), SOD, GPx, and CAT were obtained from Cayman Chemical Co. (Ann Arbor, MI, USA). The AIN-76A diet was obtained from Teklad Lab Animal Diets; Harlan (Madison, WI, USA). All other chemicals were of reagent grade.
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2

Oxidative Stress and Inflammatory Markers Assay

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DRG were dissociated with a mortar and pestle in tissue extraction reagent supplied with protease and phosphatase inhibitors as previously described.27 (link),28 (link) Assay kits for glutathione (703002, Cayman Chemical, Ann Arbor, MI, USA) (detection range: 0.25–16 μM) superoxide dismutase (706002, Cayman Chemical) (detection range: 0.005–0.050 U/ml), DNA/RNA oxidative damage (589320, Cayman Chemical) (detection range: 10.3–3,000 pg/ml), IL-1β (RLB00, R&D Systems, Minneapolis, MN) (detection range: 5–2,000 pg/ml), TNF (RTA00, R&D Systems) (detection range: 5–800 pg/ml), and CCL2 (ab100778, Abcam) (detection range: 24.67–18,000 μM), were used according to manufacturer instructions. Results were normalized to total protein levels (Bradford protein assay).
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