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3010 uhr

Manufactured by JEOL
Sourced in Japan

The JEOL 3010-UHR is a high-resolution transmission electron microscope (HRTEM) designed for advanced materials analysis. It features a 300 kV accelerating voltage and an ultra-high resolution capability, allowing for detailed imaging and analysis of nanomaterials and sub-nanometer structures.

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6 protocols using 3010 uhr

1

Structural and Morphological Alteration of Amphiboles

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The structural and morphological alteration induced on the two fibrous amphiboles by leaching in the hydrogen peroxide buffered solution were monitored after 24 h, 48 h and 1 month. Characterisation of pristine and incubated samples after one week are reported in previous works17 (link),18 (link). Samples were investigated by JEOL 3010-UHR High-Resolution Transmission Electron Microscopy (HR-TEM) with a LaB6 filament operated at 300 KeV, beam current 114 µA and equipped with a 2 K × 2 K pixels Gatan US1000 CCD camera. Elemental analysis was performed by Oxford INCA X-ray energy dispersive spectrometer (X-EDS) with a Pentafet Si(Li) detector. The fibres were dispersed in ultrapure MilliQ water, briefly sonicated to improve particle dispersion and deposited on Lacey Carbon Cu grids.
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2

Biomimetic Hydroxyapatite Nanocrystal Synthesis

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Biomimetic HA nanocrystals were produced as described by Nocerino et al.44 (link) Briefly, HA nanocrystals were precipitated from an aqueous solution of (CH3COO)2Ca (75 mM) by slow addition (one drop per second) of an aqueous solution of H3PO4 (50 mM), keeping the pH constant at 10 (by the addition of (NH4)OH solution). The synthesis was performed at room temperature. After this last process, the suspension of HA was washed with distilled water in order to remove ammonium ions and favor the interaction between nanocrystals.
Transmission electron microscopy (TEM) investigation was carried out using a 1200 EX microscope, linked to X-ray analysis detectors and a 3010 UHR operating at 300 kV (JEOL Ltd, Tokyo, Japan). Few droplets of the samples (in ultrapure water) were deposited on perforated carbon foils supported on conventional copper microgrids. The surface area was determined using a Sorpty 1750 instrument (Carlo Erba Reagents S.r.l., Milan, Italy) using N2 absorption at 77 K.45
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3

Characterization of Nanostructured Materials

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Transmission electron microscopy investigations were carried out using a 1200 EX microscope fitted with link elemental dispersive X-ray analysis detectors and a 3010 UHR operating at 300 kV (JEOL Ltd, Tokyo, Japan). The powdered samples were ultrasonically dispersed in ultrapure water and a few droplets of the slurry were then deposited on perforated carbon foils supported on conventional copper microgrids. Scanning electron microscopy observations were carried out using an 840A microscope (JEOL Ltd). The specimens were mounted on aluminum stubs using carbon tape and covered with a coating of Au-Pd approximately 10 nm thick using a coating unit (Polaron Sputter Coater E5100, Polaron Equipment, Watford, UK).
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4

High-Resolution TEM Analysis of Quartz

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To inspect the crystal lattice and the defectiveness of the quartz samples, high resolution transmission electron microscopy was carried out with a JEOL 3010-UHR, equipped with a LaB6 filament operating at 300 kV, beam current 114 μA and with a 2 k × 2 k pixels Gatan US1000 CCD camera. Quartz samples were dispersed in ultrapure water, sonicated for 20 min and a droplet was deposited on lacey carbon Cu grids.
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5

Characterization of Nanostructured Materials

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Transmission electron microscopy investigations were carried out using a 1200 EX microscope fitted with link elemental dispersive X-ray analysis detectors and a 3010 UHR operating at 300 kV (JEOL Ltd, Tokyo, Japan). The powdered samples were ultrasonically dispersed in ultrapure water and a few droplets of the slurry were then deposited on perforated carbon foils supported on conventional copper microgrids. Scanning electron microscopy observations were carried out using an 840A microscope (JEOL Ltd). The specimens were mounted on aluminum stubs using carbon tape and covered with a coating of Au-Pd approximately 10 nm thick using a coating unit (Polaron Sputter Coater E5100, Polaron Equipment, Watford, UK).
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6

High-Resolution Imaging of Mucin-GNP Complex

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High-resolution transmission electron micrographs were obtained with a JEOL 3010-UHR instrument operating at 300 kV, equipped with a 2k × 2k pixels Gatan US1000 CCD digital camera.
Samples of purified mucin-GNPs complex were dried on a carbon-coated copper grid. The nominal magnification used to record the complex mucin-GNPS were x500000 and x800000.
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