Log In Sign up
The largest database of trusted experimental protocols

Spf eggs

Manufactured by Charles River Laboratories
Visit Supplier
Sourced in Germany, United States

SPF eggs are a laboratory product designed for specific-pathogen-free (SPF) research. They provide a controlled and standardized source of eggs that are free from common avian pathogens, ensuring a reliable and consistent substrate for various scientific applications.

Automatically generated - may contain errors

Lab products found in correlation

E 410, by Olympus (1 mentions) Szx10, by Olympus (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Neomycin, by Merck Group (1 mentions)

Close spelling variants of this product

SPF) embryonated chicken eggs (11 citations) SPF) chicken eggs (4 citations) SPF 10-day-old chicken embryonated eggs (2 citations) SPF) white leghorn eggs (2 citations)

5 protocols using spf eggs

1

CAM-based Xenograft Tumor Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The CAM-assay was performed as described elsewhere [69 (link)] with slight modifications. In brief, fertilized white leghorn chicken eggs (SPF eggs) were purchased from Charles River (Germany) and incubated at 37° C with 80% humidity (Grumbach BSS 160 MPGTFS) for three days. At day three, eggs were opened and transferred to plastic weighing boats. Ex ovo cultures were covered with a square petri dish and placed into a stationary incubator at 37°C and 80% humidity for six days. Then, collagen-onplants with PC3 cells were applied to CAMs (four equal onplants/CAM) and incubated for five days. Xenografts were analyzed under a stereomicroscope with a connected digital camera and flexible cold light (Olympus SZX10, Olympus E410). For histological analysis onplants were excised from the CAM, fixed in 4% paraformaldehyde solution and processed for paraffin sectioning. To determine the tumor area the ImageJ program (NIH,USA) was used. Successful target gene overexpression or downregulation was analyzed by qPCR as described above.
Heidegger I., Kern J., Ofer P., Klocker H, & Massoner P. (2014). Oncogenic functions of IGF1R and INSR in prostate cancer include enhanced tumor growth, cell migration and angiogenesis. Oncotarget, 5(9), 2723-2735.
+ Open protocol
+ Expand
2

Salmonella-Free Hatching and Rearing

Check if the same lab product or an alternative is used in the 5 most similar protocols
SPF eggs were purchased from Charles River Laboratories and incubated until the hatched at the VIDO animal facility. All the birds were raised at the VIDO-animal care facility in direct accordance with guidelines drafted by the University of Saskatchewan's Animal Care Committee (UACC) under Canadian Council on Animal Care (CCAC). On the day of hatch, swabs were collected to check for any Salmonella contamination before transferring the animals to separate rooms. The birds were not caged and had access to litter. Ad libitum feed and water were provided.
Wellawa D.H., Lam P.K., White A.P., Allan B, & Köster W. (2022). Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection. Frontiers in Veterinary Science, 9, 948448.
+ Open protocol
+ Expand
3

Maintaining Specific Pathogen-Free Chickens

Check if the same lab product or an alternative is used in the 5 most similar protocols
White leghorn chickens hatched from SPF eggs (Charles River, North Franklin, CT) were maintained in Horsfall-type isolators in biosafety level 2 facilities. Experimental procedures and animal care were performed in compliance with all applicable federal and institutional animal guidelines. Auburn University College of Veterinary Medicine is an Association for Assessment and Accreditation of Laboratory Animal Care-accredited institution.
Eldemery F., Joiner K.S., Toro H, & van Santen V.L. (2017). Protection against infectious bronchitis virus by spike ectodomain subunit vaccine. Vaccine, 35(43), 5864-5871.
+ Open protocol
+ Expand
4

Influenza Virus Propagation and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Influenza virus A/California/04/2009 was propagated and harvested from allantoic fluid using fertilized research grade specific pathogen free (SPF) eggs (Charles River Laboratories, Norwich, CT) following standard protocols.35 Propagated virus was quantified via plaque assays using standard methods.35 Briefly, MDCK cells were infected using 10-fold dilutions then overlaid with 0.7% agarose and incubated for 7 days followed by staining with 0.5% crystal violet staining in 30% MeOH. Plaques were then counted and quantified to determine viral titers.
Tran A., Monreal I.A., Moskovets E., Aguilar H.C, & Jones J.W. (2021). Rapid Detection of Viral Envelope Lipids Using Lithium Adducts and AP-MALDI High-Resolution Mass Spectrometry. Journal of the American Society for Mass Spectrometry, 32(9), 2322-2333.
+ Open protocol
+ Expand
5

Generation and Characterization of Influenza LAIV Viruses

Check if the same lab product or an alternative is used in the 5 most similar protocols
A/Leningrad/134/17/57 (H2N2) (Len/17-MDV) was provided by BioDiem (Australia). LAIV viruses A/Texas/50/2012(H3N2)-CDC-LV4A (LV4A), A/South Africa/3646/2013(H1N1pdm09)-CDC-LV14A (LV14A), A/Hong Kong/4801/2014(H3N2) CDC-LV15A (LV15A) were generated using classical reassortment with Len/17-MDV in specific pathogen free (SPF) eggs (Charles River Laboratories Inc., USA). The viruses used in plaque assay, RG-1 (7:1 reassortant) and RG-2 (6:2 reassortant) possess HA only or HA and NA of A/Texas/50/2012 origin, respectively and the rest of the genes from Len/17-MDV. RG viruses were generated by reverse genetics as described in (Shcherbik et al., 2015 ). 293T, human embryonic kidney (HEK) and Madin-Darby canine kidney (MDCK London) cells were maintained in DMEM High Glucose (Life Technologies, Carlsbad, CA) supplemented with 10% (for HEK) or 5% (for MDCK) fetal bovine serum (Life Technologies), 1 × GlutaMAX (Life Technologies) and 40 μg/ml Neomycin (Sigma-Aldrich, St. Louis, MO). Ferret polyclonal antisera to recombinant HA protein of A/Japan/305/1957 (H2N2) was obtained from the International Reagent Resource (IRR, Manassas, VA).
Shcherbik S., Carney P., Pearce N., Stevens J., Dugan V.G., Wentworth D.E, & Bousse T. (2018). Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines. Virology, 522, 65-72.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!