Iron(II) chloride tetrahydrate, iron(III) chloride hexahydrate, and bovine serum albumin lyophilized powder were purchased from Merck KGaA, Germany. Ammonia, 25% (v/v) water solution, (3-aminoprpyl)triethoxysilane (APTES), hydrochloric acid concentrated, nitric acid, sodium hydroxide, hydrochloric acid 1N, and boric acid were purchased from Carl Roth GmbH + Co, KG, Germany. N-Succinimidyl bromoacetate was supplied by VWR International GmbH, Germany. Viral proteins and antibodies were purchased from Sino Biological, China. Deionized water was produced using a Merck Milli-Q purification system. All reagents were used without further purification.
Boric acid
Manufactured by Carl Roth
Sourced in Germany
Boric acid is a chemical compound with the formula H3BO3. It is a white, crystalline solid that is commonly used in various laboratory applications. Boric acid serves as a buffer, antiseptic, and flame retardant. Its core function is to maintain pH levels and provide a stable chemical environment for various experiments and processes.
Automatically generated - may contain errors
Lab products found in correlation
Iron 3 chloride hexahydrate, by Merck Group (2 mentions)
Iron 2 chloride tetrahydrate, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Carl Roth (2 mentions)
N succinimidyl bromoacetate, by Avantor (2 mentions)
Methanol, by Carl Roth (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Milli q purification system, by Merck Group (1 mentions)
Bovine serum albumin lyophilized powder, by Merck Group (1 mentions)
Nitric acid, by Carl Roth (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Blue dextran, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Low melting point agarose, by Merck Group (1 mentions)
Formamide, by Merck Group (1 mentions)
Leupeptin, by Merck Group (1 mentions)
Aprotinin, by Merck Group (1 mentions)
Sodium chloride (nacl), by Carl Roth (1 mentions)
Sodium orthovanadate, by Merck Group (1 mentions)
Pepstatin, by Merck Group (1 mentions)
1 4 diazabicyclo 2.2.2 octane, by Merck Group (1 mentions)
8 protocols using boric acid
1
Functionalized Iron Nanoparticle Synthesis
2
SDS-PAGE Protein Analysis Protocol
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2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris), Tris hydrochloric acid (HCl), sodium chloride (NaCl), sodium hydroxide (NaOH), disodium ethylenediaminetetraacetate (Na2EDTA), acrylamide solution, urea, boric acid, N,N,N′,N′-tetramethylethylenediamine (TEMED), ammonium persulfate (APS), dimethyl sulfoxide (DMSO), methanol (MeOH), and normal melting point agarose were obtained from Roth (Karlsruhe, Germany). Acetylated bovine serum albumin (BSA), phenylmethanesulfonyl fluoride (PMSF), aprotinin, leupeptin, pepstatin, sodium fluoride (NaF), sodium orthovanadate (Na3VO4), dithiothreitol (DTT), APC, bicinchoninic acid (BCA) solution, formamide, blue dextran, Triton X-100, low melting point agarose, 1,4-Diazabicyclo[2.2.2]octane, potassium chloride (KCl), and potassium phosphate (KH2PO4) were obtained from Sigma/Merck (Darmstadt, Germany).
3
Quantification of Glycation Adducts
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LC-MS grade methanol and acetonitrile were obtained from Fisher Chemical (Loughborough, UK). Pepsin, pronase E, sulfuric acid and creatinine were from Merck (Darmstadt, Germany), and CML and [ 2 H 2 ]CML were obtained from PolyPeptide Group (Strasbourg, France). 2-Amino-2-(hydroxymethyl)propane-1, 1-diole-hydrochloride (TRIS-HCl) was purchased from Serva Feinbiochemica (Heidelberg, Germany). Nonafluoropentanoic acid (NFPA), hydrochloric acid, prolidase, leucine aminopeptidase and n-heptane were from Sigma-Aldrich (Seelze, Germany). Lithium citrate buffer, lithium citrate/borate buffer, lithium hydroxide and ninhydrin were purchased from Sykam (Fürstenfeldbruck, Germany). Wieninger's catalyst was obtained from Honeywell (Seelze, Germany). Boric acid was purchased from Carl Roth (Karlsruhe, Germany) and sodium hydroxide from Grüssing (Filsum, Germany). Water was distilled (twice) before analysis.
Pyrraline [11] and MG-H1 [12] were synthesized according to the specified literature. The synthesis of [ 13 C 6 , 15 N 2 ] pyrraline and [ 13 C 6 ]MG-H1 was performed in the same manner but using [ 13 C 6 , 15 N 2 ]lysine (pyrraline) and [ 13 C 6 ]arginine (MG-H1) instead of the unlabeled amino acids.
Pyrraline [11] and MG-H1 [12] were synthesized according to the specified literature. The synthesis of [ 13 C 6 , 15 N 2 ] pyrraline and [ 13 C 6 ]MG-H1 was performed in the same manner but using [ 13 C 6 , 15 N 2 ]lysine (pyrraline) and [ 13 C 6 ]arginine (MG-H1) instead of the unlabeled amino acids.
4
Biomolecular Extraction and Purification
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Agarose, boric acid, isopropanol, and tris were obtained from Roth (Carl Roth GmbH and Co. KG, Karlsruhe, Germany). Bovine serum albumin, ethanol, and tRNA were purchased from Sigma-Aldrich (St. Louis, MI, USA). EDTA and Dulbecco’s Phosphate-Buffered Saline (DPBS) (1x) were ordered from Thermo Fisher Scientific (Waltham, MA, USA).
5
Antioxidant Capacity Assessment Protocol
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Ammoniac (NH3), ammonium acetate, boric acid, ferulic acid, hydrochloric acid (HCl), kaempferol-3-O-glucoside, methanol, natrium hydroxide (NaOH), quercetin-3-O-glucoside, sinapic acid, and tert-butyl methyl ether were obtained from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). Acetic acid, 2-propanol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), methylene chloride, potassium persulfate, and Trolox® (6-hydroxyl-2,5,7,8-tetramethylchroman-2carbonic acid) were purchased from Merck KGaA (Darmstadt, Germany). 2,2′-Azinobis-(3-ethylbenzthiazoline-6sulfonic acid) (ABTS) was purchased from Roche Diagnostic GmbH (Mannheim, Germany) and sulfuric acid from Grüssing GmbH (Filsum, Germany). Acetonitrile was obtained from J.T. Baker (Fisher Scientific GmbH, Grießheim, Germany) and tetrahydrofurane was from VWR International GmbH (Darmstadt, Germany). The standards of β-carotene, chlorophyll a, chlorophyll b, lutein, and zeaxanthin were purchased from CaroteNature® GmbH (Münsingen, Switzerland) and pheophytin a from LGC Standards GmbH (Wesel, Germany).
6
Peptide-based Bioconjugation Protocol
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The deionized H2O used in the experiments was prepared using the Merck Milli-Q® direct water purification system (Darmstadt, Germany). The iron calibration standard was obtained from Bernd Kraft (Duisburg, Germany). Merck (Darmstadt, Germany) supplied iron (II) chloride tetrahydrate, iron (III) chloride hexahydrate, Calcium chloride, and Tween 20. Ringer solution was obtained from Fresenius Kabi (Bad Homburg, Germany). RPMI 1640 medium supplemented with 10% fetal calf serum (FCS) and 1% L-glutamine was obtained from Live Technologies (Carlsbad, CA, USA). N-Succinimidyl bromoacetate was supplied by VWR International GmbH (Damstadt, Germany). Calcium chloride, boric acid, tris-(hydroxymethyl)-aminomethane, N,N-dimethylformamide, 3-aminopropyltriethoxysilan, ammonia (25% v/v solution), and hydrochloric acid (HCl) were supplied by Carl Roth GmbH (Karlsruhe, Germany).
The peptides PepA (RKQGRVEVLYRASWGTVC), PepB (RKQGRVEILYRGSWGTVC), PepC (RKQGRVEVLYRASWGTVS-℗ ) (℗ = phosphoserine), PepD (RKQGRVEVIYRGSWGTVC), and PepE (RKQGRAEALYRASWGTVC) were supplied by Proteogenix (Schiltigheim, France) with a purity > 85%.
The peptides PepA (RKQGRVEVLYRASWGTVC), PepB (RKQGRVEILYRGSWGTVC), PepC (RKQGRVEVLYRASWGTVS-
7
Chemical Reagents for Analytical Experiments
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All chemicals used were of analytical grade and used as purchased without further treatment. Boric acid, iron(iii ) nitride, sodium oxalate, potassium bromate, and hydrogen peroxide were obtained from CARL ROTH GmbH (Karlsruhe, Germany). Ammonia at 25% was purchased from Merck (Darmstadt, Germany). Isopropyl alcohol was purchased from Fluka AG in Sigma Aldrich (Steinheim, Germany). Ascorbic acid was purchased from Scharlau Chemicals (Barcelona, Spain) and folic acid was purchased from UNI-CHEM (China) chemicals.
8
Milk Sampling Methodology for Microbiological Analysis
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During each visit, quarter foremilk samples were collected aseptically from all lactating cows during the afternoon milking according to the German Veterinary Association guidelines (GVA, 2018) . First, teat ends were disinfected with disposable wipes soaked with ethanol (70%); then, 3 milk streams were discarded before the milk was sampled into a sterile 13-mL tube containing a boric acid-based preserving agent (Ly-20; Heeschen et al., 1969) . The Ly-20 was prepared using 50.0 g of boric acid (Carl Roth), 0.75 g of potassium sorbate (SigmaAldrich), 10 g of water-free glycerin (Carl Roth), 0.5 g of methylene blue (1%, SigmaAldrich), and 1,000.0 mL of distilled water. Immediately after milking, samples were transported cooled to the laboratory (University of Applied Sciences and Arts, Hannover, Germany), and microbiological analysis commenced within 18 h after sampling.
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