Nis elements br ver 4

For histological observation, 0.7 cm pieces of stem in the middle of the internodes were cut 1 cm above the point of the application of the lanolin paste of IAA at 0.1% and JA-Me at 0.5% on the sixth, the eighth and the ninth day after treatment. Five samples of shoots were collected for each treatment. Pieces of the stem internode treated with lanolin only, IAA at 0.1% and JA-Me at 0.5% were collected. The materials were fixed in a chromic acid, acetic acid and formalin (CrAF) solution for 48 h at room temperature, dehydrated through an increasing alcohol series (70%, 80%, 90% and 100%), and embedded in paraffin. Longitudinal sections, 15 μm thick, were cut with a rotary microtome (Leica, Wetzelar, Germany) and stained with safranin (1% prepared in ultrapure water) followed by fast green (1% prepared in 95% ethanol). The sections were mounted in Canada balsam and analysed using a light microscope (Eclipse 80i, Nikon, Tokyo, Japan) with imaging software NIS-Elements BR ver. 4.00 (Nikon Instruments Inc., Tokyo, Japan) for photo documentation.