PM
2.5 was collected outdoors from November 2019 to February 2020. The average temperature and humidity during the sampling period are shown in
Table 1. PM
2.5 sampler (Dandong Better Instruments Co., Heilongjiang, China) was set at the Chinese Research Academy of Environmental Sciences, Beijing. The sampling flow rate was 16.67 L/min. PM
2.5 was collected with Teflon membrane. Teflon membrane equilibrate in a constant temperature and humidity (20~25 °C, 50% humidity) box prior to this collection for 24 h, weigh the membrane with a 0.01 mg electronic scale (
MS105 Mettler Toledo, Zurich, Switzerland) prior to sampling, weigh again after sampling, and record the difference in membrane weights. The collecting sampled membranes were stored at −20 °C until analysis. Each membrane is collected for 23 h (4:00 p.m. to 3:00 a.m. the next day).
The following is the process of PM
2.5 suspension preparation. The Teflon membranes loaded with PM
2.5 were ultrasonically extracted with ultrapure water. The mixture was then freeze-dried, and the solids were accurately weighed using a one ten-thousandth balance. Finally, the solids were mixed with DMEM medium without serum to form PM
2.5 suspension. PM
2.5 suspension was stored at 4 °C and protected from light before use.
Wu T., Liu B., Wei Y, & Li Z. (2023). TGF-β Regulates m6A RNA Methylation after PM2.5 Exposure. Toxics, 11(12), 1026.