Labospect 003

Manufactured by Hitachi

Sourced in Japan

The Labospect 003 is a compact and versatile laboratory equipment designed for a variety of analytical tasks. It features a high-resolution display, intuitive controls, and precise measurement capabilities. The Labospect 003 is suitable for use in various laboratory settings, providing reliable and accurate results.

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Lab products found in correlation

Celltac α, by Nihon Kohden (2 mentions) Synergy 2 multi mode microplate reader, by Agilent Technologies (1 mentions) Qualigent, by Sekisui (1 mentions) Fgf 21 elisa kit, by BioVendor (1 mentions) Rm2245 microtome, by Leica (1 mentions) Spac s aldosterone, by Fujirebio (1 mentions)

7 protocols using labospect 003

Quantifying Blood Cell Parameters

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The levels of RBCs, WBCs, HCT, and PLTs were quantified using an automated cell counter (Celltac α, Nihon Kohden Co., Ltd., Tokyo, Japan). Blood Alb was
measured using an automatic analyzer after the sample was centrifuged at 1,500 rpm for 5 min (LABOSPECT 003, Hitachi High-Technologies Corp, Tokyo, Japan).
Blood samples for cytokine measurement were centrifuged at 1,000 × g for 30 min at room temperature within 30 min of blood collection. The
plasma was frozen and immediately stored below −20°C. Blood samples were thawed at room temperature before undertaking measurements. Enzyme-linked immunosorbent
assays were conducted using Quantikine Canine Immunoassay kits (R&D Systems, Minneapolis, MN, USA) for IL-6, IL-8, IL-10, and TNF-α. All measurements were
performed using a spectrophotometer (Synergy 2 Multi-Mode Microplate Reader; BioTek Instruments Inc., Winooski, VT, USA).

SUZUKI H., OSHIMA N, & WATARI T. (2020). Effect of modified ultrafiltration on cytokines and hemoconcentration in dogs undergoing cardiopulmonary bypass. The Journal of Veterinary Medical Science, 82(11), 1589-1593.

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Comprehensive Metabolic Profile Analysis

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Serum total cholesterol (TC), TG, glucose, non-esterified fatty acids (NEFA), AST, ALT, phospholipids (PL), and creatinine (CRN) levels were determined using a Labospect 003 autoanalyzer (Hitachi High-Technologies Corporation, Tokyo, Japan).

Murakami K., Sasaki Y., Asahiyama M., Yano W., Takizawa T., Kamiya W., Matsumura Y., Anai M., Osawa T., Fruchart J.C., Fruchart-Najib J., Aburatani H., Sakai J., Kodama T, & Tanaka T. (2022). Selective PPARα Modulator Pemafibrate and Sodium-Glucose Cotransporter 2 Inhibitor Tofogliflozin Combination Treatment Improved Histopathology in Experimental Mice Model of Non-Alcoholic Steatohepatitis. Cells, 11(4), 720.

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Serum Biomarkers Quantification Protocol

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Serum levels of triglyceride, total cholesterol, β-hydroxybutyrate, NEFA, and FGF21 were measured according to the manufacturer’s instruction. Briefly, after blood sample collection, serum samples were enzymatically processed with Qualigent (triglyceride and total cholesterol, Sekisui Medical, Tokyo, Japan), 3HB-L Test Kit (β-hydroxybutyrate, Kainos, Tokyo, Japan), and Clinimate NEFA (NEFA, Sekisui Medical, Tokyo, Japan). Then, they were measured using the Hitachi Automated Analyzer (Labospect003, Hitachi High-Technologies, Tokyo, Japan). Serum FGF21 level was measured using an FGF-21 ELISA kit (Cat #RD291108200R, BioVendor Laboratory Medicine, Brno, Czech Republic).

Tomita Y., Lee D., Miwa Y., Jiang X., Ohta M., Tsubota K, & Kurihara T. (2020). Pemafibrate Protects Against Retinal Dysfunction in a Murine Model of Diabetic Retinopathy. International Journal of Molecular Sciences, 21(17), 6243.

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Comprehensive Metabolic Profiling Protocol

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Serum levels of total cholesterol, TG, glucose, free fatty acids, AST, ALT, phospholipids, and creatinine were determined using a Labospect 003 autoanalyzer (Hitachi High-Technologies Corporation, Tokyo, Japan).

Sasaki Y., Asahiyama M., Tanaka T., Yamamoto S., Murakami K., Kamiya W., Matsumura Y., Osawa T., Anai M., Fruchart J.C., Aburatani H., Sakai J, & Kodama T. (2020). Pemafibrate, a selective PPARα modulator, prevents non-alcoholic steatohepatitis development without reducing the hepatic triglyceride content. Scientific Reports, 10, 7818.

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Metallic Implant Biocompatibility Assessment

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Venous blood specimens from six rabbits in the experimental group were collected at six time points, including immediately preoperatively and 7, 14, 30, 60 and 90 days postoperatively. The serum concentrations of Mg²⁺, Zn²⁺, and Ca²⁺ ions as well as blood urea nitrogen (BUN), creatinine (Cr), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were measured by a biochemical analyzer (LABOSPECT 003, Hitachi).
Four animals were sacrificed by overdose of pentobarbital sodium at each observation point (4, 8 and 12 weeks after implantation). The heart, liver, kidney, and bone tissue surrounding the implants were harvested. These specimens were fixed in 4% paraformaldehyde for 48 h. In addition, the bone tissues were decalcified with 10% ethylene diamine tetraacetic acid for 30 days. The specimens were dehydrated with gradient ethyl alcohol solutions and embedded in paraffin. A Leica RM 2245 microtome was utilized to cut the tissue into sections of 5 μm thickness. The sections were stained sequentially with hematoxylin and eosin (H&E). Radiographs of the implantation area were obtained at 4, 8, and 12 weeks postoperatively for in vivo observation of the degradation process, release of hydrogen, and bone regeneration process.

Liu C., Wang J., Gao C., Wang Z., Zhou X., Tang M., Yu K, & Deng Y. (2020). Enhanced osteoinductivity and corrosion resistance of dopamine/gelatin/rhBMP-2–coated β-TCP/Mg-Zn orthopedic implants: An in vitro and in vivo study. PLoS ONE, 15(1), e0228247.

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Blood Sample Hematological Analysis

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All blood samples obtained were mixed with 15% ethylenediaminetetraacetic acid (EDTA)–dipotassium anhydrate before hematological examination was conducted with the Celltac α automatic blood cell counter (Nihon Kohden Corporation, Tokyo, Japan). Plasma was separated from the blood, and AST and ALT levels were determined with an automatic analyzer (LABOSPECT 003, Hitachi Ltd, Tokyo, Japan).

Yanagiba Y., Suzuki T., Suda M., Hojo R., Gonzalez F.J., Nakajima T, & Wang R.S. (2015). Cytochrome P450 2E1 is responsible for the initiation of 1,2-dichloropropane-induced liver damage. Toxicology and industrial health, 32(9), 1589-1597.

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Radioimmunoassay for Plasma Renin and Aldosterone

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Both PRA and PAC were measured using a radioimmunoassay kit (SRL renin kit; FUJIREBIO Inc, Tokyo, Japan and Spac‐S aldosterone; FUJIREBIO Inc) in accordance with the manufacturer's instructions.13, 14 All samples were analyzed in duplicate by personnel blinded to dog identity, and the mean values of the data obtained were used for analyses. Heparinized plasma was tested immediately after centrifugation. Liver enzyme activity, and albumin, bilirubin, blood urea nitrogen, creatinine, fasting serum bile acid, ammonia, sodium, potassium, and chloride concentrations were measured using a biochemical auto‐analyzer (LABOSPECT 003; Hitachi High‐Technologies Co, Ltd, Tokyo, Japan).

Sakamoto Y., Sakai M., Sato K, & Watari T. (2019). Plasma renin activity and aldosterone concentration in dogs with acquired portosystemic collaterals. Journal of Veterinary Internal Medicine, 34(1), 139-144.

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