Branson ultrasonic cleaner

Manufactured by Emerson

Sourced in United States

The Branson Ultrasonic Cleaner is a laboratory equipment designed for cleaning a variety of items. It utilizes ultrasonic waves to agitate a cleaning solution, allowing for the removal of contaminants from the target objects.

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Lab products found in correlation

Nitric acid, by Thermo Fisher Scientific (1 mentions) Analog vortex mixer, by Avantor (1 mentions)

3 protocols using branson ultrasonic cleaner

Quantifying Stent Encrustation Composition

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All experimental containers were prewashed with
1% nitric acid (Fisher Scientific, Hampton, NH) before ultra-trace
elemental analysis via inductively coupled plasma mass spectrometry
(ICP-MS). 11 week dried, encrusted stent specimens were submerged
in 1 mL of 1% HNO₃ (v/v), followed by ultrasonication for
1 h using a Branson ultrasonic cleaner (Branson Ultrasonics Corp.,
Danbury, CT) to agitate, extract, and dissolve the encrusted minerals
on the stents. Each sonicated sample was further diluted 100-fold
in 1% HNO₃ to achieve a total dissolved solid of <1
weight percent (wt %) and a final volume of 10 mL before ICP-MS analysis.
The presence of magnesium (Mg), calcium (Ca), and phosphorus (P) was
measured by using an iCAP RQ inductively coupled plasma mass spectrometer
with a Teledyne laser ablation (LA) system (Thermo Scientific, Waltham,
MA, USA). Standard solutions of Mg, Ca, and P (Inorganic Ventures,
Christiansburg, VA, USA) were used to generate a standard curve (100,
50, 20, and 0 ppm). One-way ANOVA was performed using GraphPad Prism
(Dotmatics, Boston, MA, USA) to compare the amount of encrusted deposit
on each stent material.

Wu Y.X., Choi E.J., Vu A.A., Jiang P., Ali S.N., Patel R.M., Landman J, & Clayman R.V. (2023). Comparison of Ureteral Stent Biomaterials: Encrustation Profile in Lithogenic Artificial Urine Models. ACS Omega, 8(32), 29003-29011.

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Minimal Biofilm Eradication Concentration Assay

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The minimal biofilm eradication concentration (MBEC) assay was performed in 96-well polystyrene plates to evaluate the effect of antimicrobials on mature biofilm. Briefly, yeast cell suspensions at a 10⁷ cfu/mL density were inoculated into wells containing TSB-glucose (TSB-g) at 37 °C for 24 h. After the incubation, each well was washed thrice with 200 μL of sterilized PBS. Then, fresh medium containing various concentrations of antimicrobials (20–1280 μg/mL) was added to each well of the 96-well microtiter plate, followed by further incubation at 37 °C for 24 h. After exposure of antifungals to the biofilm, each well was gently washed three times with PBS. After adding PBS to all wells, the biofilm was detached by sonication in an ultrasonic cleaner (Branson Ultrasonic Cleaner; Branson Ultrasonics, Danbury, CT, USA) and vortexed. To determine CFU/mL, samples were taken from wells, diluted in PBS, and dropped onto SDA. After overnight incubation, colonies were counted. MBEC_99.9 was defined as the lowest concentration to eradicate at least 99.9% of viable bacteria (a three-log reduction) in a biofilm compared to the growth controls.

Oyardi O., Demir E.S., Alkan B., Komec S., Genc G.E., Aygun G., Teke L., Turan D., Erturan Z., Savage P.B, & Guzel C.B. (2023). Phenotypic Investigation of Virulence Factors, Susceptibility to Ceragenins, and the Impact of Biofilm Formation on Drug Efficacy in Candida auris Isolates from Türkiye. Journal of Fungi, 9(10), 1026.

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EV Degradation Optimization Protocol

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EVs were exposed to the following conditions to determine the amount of degradation. Vortexing: 50 µL of EVs were continuously vortexed for 5 minutes at 2000 rpm using an analog vortex mixer (VWR, Bohemia, NY, USA). Sonication: 50 µL of EVs were placed in a Branson Ultrasonic Cleaner (Model 3510, Branson Ultrasonics Corporation, USA) for 10 min at room temperature. Freeze-thaw cycles: 50 µL of EVs were exposed to 10 freezethaw cycles. This was performed by freezing the samples at -80 • C for 30 seconds and then allowing them to thaw at room temperature for 2.5 min completely. Three technical replicates from each concentration were tested and described in supplementary materials. The optimization of degradation conditions was described in supplementary materials.

Dou Y., Ren L., Kulabhusan P.K., Zaripov E., & Berezovski M.V. (2021). Quantitative Capillary Electrophoresis for Analysis of Extracellular Vesicles (EVqCE). Separations, 8(8), 110-110.

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