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Donkey anti goat alexa 647

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Donkey anti-goat Alexa 647 is a secondary antibody conjugated with Alexa Fluor 647 dye. It is designed to detect and bind to primary antibodies raised in goat, allowing for fluorescent labeling and visualization of target proteins or antigens.

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Lab products found in correlation

Ab144p, by Merck Group (1 mentions) Fv10i, by Olympus (1 mentions) Triton x 100, by Merck Group (1 mentions) Lsm 880 nlo microscope, by Zeiss (1 mentions) Goat anti iba 1, by Novus Biologicals (1 mentions) Donkey anti mouse alexa 488, by Jackson ImmunoResearch (1 mentions) C9100 02, by Hamamatsu Photonics (1 mentions) Stereo investigator, by MicroBrightField (1 mentions)

Spelling variants of this product

Alexa 647 (67 citations) Donkey anti-goat Alexa Fluor 647 (8 citations) Donkey Anti-Goat 647 (2 citations) Alexa Fluor 647–conjugated donkey anti-goat IgG (2 citations) Alexa Fluor 647 AffiniPure Donkey Anti-Goat (2 citations)

3 protocols using donkey anti goat alexa 647

1

ChAT Immunofluorescence of Mouse Brain Sections

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We employed the following protocol of ChAT immunofluorescence for mouse brain sections (30 μm): first, permeabilization with PBS-TritonX-100 (1 h at room temperature, mild shaking); next, blocking in PBS-TritonX-100/5% Normal Donkey Serum (NDS; 1 h); then, incubation of the sections in primary antibody anti-ChAT 1:150 (Ab144p, MilliporeSigma, Burlington, MA) in PBS with Tween20/3% NDS (over 3 nights at 4°C, mild shaking); and last, incubation of the sections in the secondary antibody donkey anti-goat Alexa 647 1:500 (Jackson ImmunoResearch, West Grove, PA; 2 h at room temperature, mild shaking). We mounted the sections and imaged them with a FV-10i (Olympus, Tokyo, Japan) confocal microscope.
Dudai A., Yayon N., Lerner V., Tasaka G.I., Deitcher Y., Gorfine K., Niederhoffer N., Mizrahi A., Soreq H, & London M. (2020). Barrel cortex VIP/ChAT interneurons suppress sensory responses in vivo. PLoS Biology, 18(2), e3000613.
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2

Immunohistochemical Profiling of Microglia

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Organotypic brain slice cultures were blocked with 5% normal donkey serum (2h) and 0.3% Triton X-100 (Sigma-Aldrich) in PBS. For microglial detection goat anti-Iba1 (1:250, Novus Biologicals) and mouse anti-STEM101 (1:250, TaKaRa) were used in 2% NDS, 0.3% Triton X-100 at 4 °C overnight. Following Alexa-fluorophore-conjugated secondary antibodies were applied in a concentration of 1:250 for 2h at RT: donkey-anti-goat Alexa-647, donkey-anti-mouse Alexa-488, (all Jackson ImmunoResearch). Slices were analyzed with a Zeiss LSM 880 NLO microscope equipped with a 20x water-immersion objective (W Plan-Apochromat x20/1.0, Carl Zeiss, Jena) (Figure S6K).
Pantazis C.B., Yang A., Lara E., McDonough J.A., Blauwendraat C., Peng L., Oguro H., Kanaujiya J., Zou J., Sebesta D., Pratt G., Cross E., Blockwick J., Buxton P., Kinner-Bibeau L., Medura C., Tompkins C., Hughes S., Santiana M., Faghri F., Nalls M.A., Vitale D., Ballard S., Qi Y.A., Ramos D.M., Anderson K.M., Stadler J., Narayan P., Papademetriou J., Reilly L., Nelson M.P., Aggarwal S., Rosen L.U., Kirwan P., Pisupati V., Coon S.L., Scholz S.W., Priebe T., Öttl M., Dong J., Meijer M., Janssen L.J., Lourenco V.S., van der Kant R., Crusius D., Paquet D., Raulin A.C., Bu G., Held A., Wainger B.J., Gabriele R.M., Casey J.M., Wray S., Abu-Bonsrah D., Parish C.L., Beccari M.S., Cleveland D.W., Li E., Rose I.V., Kampmann M., Aristoy C.C., Verstreken P., Heinrich L., Chen M.Y., Schüle B., Dou D., Holzbaur E.L., Zanellati M.C., Basundra R., Deshmukh M., Cohen S., Khanna R., Raman M., Nevin Z.S., Matia M., Van Lent J., Timmerman V., Conklin B.R., Chase K.J., Zhang K., Funes S., Bosco D.A., Erlebach L., Welzer M., Kronenberg-Versteeg D., Lyu G., Arenas E., Coccia E., Sarrafha L., Ahfeldt T., Marioni J.C., Skarnes W.C., Cookson M.R., Ward M.E, & Merkle F.T. (2022). A reference human induced pluripotent stem cell line for large-scale collaborative studies. Cell stem cell, 29(12), 1685-1702.e22.
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3

Quantifying 5-HT Neuron Activity in DRN

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To assess whether STN-DBS influenced 5-HT synthesis of eYFP expressing 5-HT DRN neurons, tissue was processed for TPH2 immunohistochemistry, which is the rate-limiting enzyme in 5-HT synthesis. DRN sections were incubated overnight with a primary anti-TPH2 antibody (goat polyclonal anti-TPH2; Abcam; 1:2000). This was followed by incubation with a secondary antibody (donkey anti-goat alexa 647, Jackson Immunoresearch Laboratories; 1:200) for two hours. Stereological analysis of double-labelled eYFP/TPH2 neurons was performed (Stereo Investigator, Microbrightfield Bioscience, Williston, VT, USA) in seven DRN sections per mouse using a fluorescence spinning disk confocal microscope (DSU, Olympus BX51, Japan) connected to a digital ultra-high sensitivity CCD camera (C9100-02, Hamamatsu Photonics, Japan). Stereological cell counting was performed using the optical fractionator probe and total double-labelled cell number was estimated using a validated stereological method43 (link),44 (link).
Alosaimi F., Temel Y., Hescham S., Witzig V.S., Almasabi F., Tan S.K, & Jahanshahi A. (2022). High-frequency stimulation of the subthalamic nucleus induces a sustained inhibition of serotonergic system via loss of cell phenotype. Scientific Reports, 12, 14011.
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