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2 protocols using hexadecyltrimethylammonium chloride ctac

1

Synthesis of Plasmonic Nanoparticles

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Cetyltrimethylammonium bromide (CTAB, ≥ 99%), gold (III) chloride trihydrate (HAuCl4·3H2O, ≥99.9%), ascorbic acid (≥99.0%), silver nitrate (AgNO3, ≥99.0%), sodium borohydride (NaBH4, 98%), 8-hydroxyquinoline (8-HQL, 99.0%), poly(sodium-p-styrenesulfonate) (PSS, average M.W. ~1,000,000), 4-aminothiophenol (p-ATP, 97%), and zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 98%) were purchased from Sigma Aldrich. Nitric Acid (HNO3, 70%), hydrogen Peroxide (H2O2, 30%), sulfuric Acid (H2SO4, 95.0 to 98.0 w/w %), phosphate buffered saline (PBS, 10X), ethylenediaminetetraacetic acid (EDTA, >99%), sucrose and sodium hydroxide (NaOH, ≥97.0%) were purchased from Fisher Chemical. Hexadecyltrimethylammonium chloride (CTAC, 99%), and 2-methylimidazole (99%) were purchased from ACROS Organics. Protein A, human IgG, mouse anti-rabbit IgG, fetal bovine serum, and goat anti-human IgG were purchased from Invitrogen. Type 1 deionized water (18.2 mΩ·cm) was used in all experiments and produced by Sartorius Arium Pro ultrapure water system. The filter paper (Whatman No. 1 grade) used in SERS experiments was purchased from Fisher Chemical.
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2

Fluorescein Reduction and Nanoparticle Synthesis

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Reagents for the reduction of fluorescein
(fluorescein sodium salt, BioReagent, suitable for fluorescence) by
borohydride (sodium borohydride, powder, ≥ 98.0%) were purchased
from Sigma-Aldrich. For nanoparticle synthesis, Au(III) chloride trihydrate
(HAuCl4·3H2O, ≥99.9%, Sigma-Aldrich),
hexadecyltrimethylammonium chloride (CTAC, 99%, Acros Organics), sodium
borohydride (NaBH4, ≥98%, Sigma-Aldrich), sodium
bromide (NaBr, ≥99%, Sigma-Aldrich), potassium iodide (KI,
≥99%, Sigma-Aldrich), l-ascorbic acid (≥99%,
Sigma-Aldrich), silver nitrate (AgNO3, ≥99.0%, Sigma-Aldrich),
polyvinylpyrrolidione (PVP, average MW = ∼55 000, Sigma-Aldrich),
sodium citrate tribasic dihydrate (Sigma-Aldrich, ≥99.0%),
acetone (AnalaR NORMAPUR, VWR), and hydrogen peroxide (H2O2, 30% w/w in H2O, Sigma-Aldrich) were used
without further purification. Ultrapure water (Milli-Q Advantage A10
water purification, Merck) was used for all nanoparticle synthesis
and washing.
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