Us il 14 18956

Manufactured by American Type Culture Collection

The US/IL/14-18956 is a laboratory equipment product. It is designed for a core function of performing specific tasks in a laboratory setting. However, detailed technical specifications or intended use cases are not available at this time.

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Lab products found in correlation

Us mo 14 18949, by American Type Culture Collection (2 mentions) Us il 14 18952, by American Type Culture Collection (2 mentions) Us ky 14 18953, by American Type Culture Collection (2 mentions) Us mo 14 18947, by American Type Culture Collection (2 mentions) Ccl 136, by American Type Culture Collection (1 mentions) Sh sy5y, by American Type Culture Collection (1 mentions)

2 protocols using us il 14 18956

Culturing and Amplification of Enteroviruses

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Rhabdomyosarcoma (RD, ATCC, CCL-136), A172 (ATCC, CRL-1620) and SH-SY5Y (ATCC, CRL-2266) were maintained in a 37 °C incubator in a 5% CO₂ atmosphere. RD cell was cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S). SH-SY5Y were cultured in 10% FBS and 1% P/S with 50% DMEM and 50% F-12 medium. All of the following EV-D68 strains used in this study were purchased from ATCC: US/KY/14-18953 (ATCC, NE-49132), US/MO/14-18947 (ATCC, NR-49129), US/MO/14-18949 (ATCC, NR-49130), US/IL/14-18952 (ATCC, NR-49131), US/IL/14-18956 (ATCC, NR-49133). All of the following EV-A71 strains used in this study were purchased from ATCC or BEI Resources: Tainan/4643/1998 (BEI Resources, NR-471), Enterovirus 71, MP4 (BEI Resources, NR-472). Enteroviruses A71 US/CT/2016- 19519 was obtained from Dr. William Nix at the Centers for Disease Control and Prevention under a material transfer agreement. All viruses were amplified in RD cells prior to infection assays.

Hu Y., Kitamura N., Musharrafieh R, & Wang J. (2021). Discovery of potent and broad-spectrum pyrazolopyridine-containing antivirals against enterovirus D68, A71, and coxsackievirus B3 by targeting the viral 2C protein. Journal of medicinal chemistry, 64(12), 8755-8774.

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Rhabdomyosarcoma and EV-D68 Virus Culture

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Rhabdomyosarcoma (RD, ATCC, CCL-136), A172 (ATCC, CRL-1620), A549 (ATCC,
CCL-185), HeLa (ATCC, CCL-2), and SH-SY5Y (ATCC, CRL-2266) were maintained in a
37 °C in a 5% CO₂ atmosphere. RD and A172 were cultured in
Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine
serum (FBS) and 1% penicillin-streptomycin (P/S). SH-SY5Y were cultured in 10%
FBS and 1% P/S with 50% DMEM and 50% F-12 medium. All of the following EV-D68
strains used in this study were purchased from ATCC: US/KY/14-18953 (ATCC,
NE-49132), US/MO/14-18947 (ATCC, NR-49129), US/MO/14-18949 (ATCC, NR-49130),
US/IL/14-18952 (ATCC, NR-49131), US/IL/14-18956 (ATCC, NR-49133).

Musharrafieh R., Zhang J., Tuohy P., Kitamura N., Bellampalli S.S., Hu Y., Khanna R, & Wang J. (2019). Discovery of quinoline analogs as potent antivirals against enterovirus D68 (EV-D68). Journal of medicinal chemistry, 62(8), 4074-4090.

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