Images were acquired using a Zeiss LSM 510 NLO and Leica 5 confocal scanning microscopes. The excitation wavelengths were 488 nm (argon laser) for Alexa 488-conjugated antibodies and GFP, and 543 nm (Helium–Neon-Laser) for Alexa 555-conjugated antibodies and propidium iodine. Emission was detected at 500–550 nm for A488-conjugated antibodies and GFP, and above 575 nm for A555-conjugated antibodies and propidium iodine. DAPI (4',6-diamidino-2-phenylindole) images were obtained using a 2-photon module with excitation at 2 × 365 nm and emission at 435–485 nm. All multi-labelled signals were detected in multi-tracking mode to avoid fluorescence crosstalk. Images were analyzed with the ZEN2010 image browser (Carl Zeiss Micro Imaging), and LEICA LAZ ES and Image J.
Lsm 510 nlo
Manufactured by Leica
The Leica LSM 510 NLO is a confocal laser scanning microscope designed for non-linear optical (NLO) imaging. It provides high-resolution imaging capabilities for applications that require multi-photon excitation, such as deep tissue imaging. The system features a tunable, femtosecond pulsed laser, allowing for efficient excitation of fluorophores and the ability to perform various NLO techniques, including two-photon fluorescence, second-harmonic generation, and third-harmonic generation imaging.
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2 protocols using lsm 510 nlo
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Multicolor Imaging Protocols for Cellular Analysis
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Multicolor Imaging Protocols for Cellular Analysis
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Images were acquired using a Zeiss LSM 510 NLO and Leica 5 confocal scanning microscopes. The excitation wavelengths were 488 nm (argon laser) for Alexa 488-conjugated antibodies and GFP, and 543 nm (Helium–Neon-Laser) for Alexa 555-conjugated antibodies and propidium iodine. Emission was detected at 500–550 nm for A488-conjugated antibodies and GFP, and above 575 nm for A555-conjugated antibodies and propidium iodine. DAPI (4',6-diamidino-2-phenylindole) images were obtained using a 2-photon module with excitation at 2 × 365 nm and emission at 435–485 nm. All multi-labelled signals were detected in multi-tracking mode to avoid fluorescence crosstalk. Images were analyzed with the ZEN2010 image browser (Carl Zeiss Micro Imaging), and LEICA LAZ ES and Image J.
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