CX-4945 was purchased from Cayman Chemical (Ann Arbor, MI, USA). RPMI 1640 (LM-011-60), fetal bovine serum (FBS) (S001-01), and cocktail of penicillin/streptomycin cocktail (LS202-02) were obtained from Welgene, Inc. (Daegu, Republic of Korea). Control siRNA (cat. no. sc-37007), CK2α siRNA (cat. no. sc-29918), HIF-1α siRNA (cat. no. sc-35561), STAT-3 siRNA (cat. no. sc-29493), and STAT-5 siRNA (cat. no. sc-29495) were purchased from Santa Cruz Biotechnology (Delaware, CA, USA). A detailed list of antibodies used in this study is included in Supplementary Table S1 . Western BrightTM Enhanced chemiluminescence (ECL, cat. no. K-12045-D20) was purchased from Advansta Corporation (San Jose, CA, USA). All the plasticwares used for cell culture were obtained from SPL Life Sciences (Gyeonggi-do, Korea).
Cx 4945
Manufactured by Cayman Chemical
Sourced in United States
CX-4945 is a small-molecule inhibitor of the enzyme CK2 (casein kinase 2). It is a white crystalline powder that is soluble in DMSO, DMF, and ethanol.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum s001 01, by Welgene (1 mentions)
Niclosamide, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by USA Scientific (1 mentions)
H1650, by American Type Culture Collection (1 mentions)
α tubulin antibody, by Cell Signaling Technology (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by AstraZeneca (1 mentions)
Selumetinib, by AstraZeneca (1 mentions)
Ms 275, by Cayman Chemical (1 mentions)
C57bl 6j male mice, by Jackson ImmunoResearch (1 mentions)
4 protocols using cx 4945
1
Detailed CK2, HIF-1α, STAT-3/5 Inhibition Protocol
2
Inhibition of CK2 by CX-4945 and TBB
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CK2 inhibitors CX-4945 (silmitasertib) and TBB (4,5,6,7-Tetrabromobenzotriazole) were purchased from Cayman Chemicals and Sigma, respectively. Niclosamide was from Sigma (Germany). Data are reported as means ± SEM. Student’s t-test (for paired or unpaired samples as appropriate) or ANOVA were used for statistical analysis. A value of p < 0.05 was accepted as a significant difference.
3
Investigating NSCLC Cell Responses to CK2 and MEK1/2 Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A549, H460, and H1650 (NSCLC) cell lines were purchased from ATCC. PC9 cells (NSCLC) were a gift from Eric Haura, MD (Moffitt Cancer Center, Tampa, FL). Cells were cultured in RPMI 1640 (Life Technologies) supplemented with 10% fetal bovine serum (FBS; USA Scientific), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), glucose, and pyruvate and maintained in a humidified incubator at 37°C in 5% CO2 unless otherwise specified. For analysis of protein content by western blot, 3 × 104 cells were plated into a 6-well dish and allowed to adhere in RPMI 1640 containing 10% FBS for 48 hours. Following the adherence period, cells were treated with the concentrations of the CK2 inhibitor, CX-4945 (Silmitasertib; Cayman Chemical), or the MEK1/2 inhibitor, AZD6244 (Selumetinib; AstraZeneca), in RPMI 1640 containing 1% FBS for the treatment durations indicated. Both adherent and nonadherent cells from each sample were collected for total protein. Ten percent of total cell extracts were loaded. Cleaved poly(ADP-ribose) polymerase (PARP) and α-tubulin antibodies were purchased from Cell Signaling.
4
Mouse Model for Epigenetic Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experimental procedures were approved by The Institutional Animal Care and Use Committee of the Cleveland Clinic and Oregon Health and Science University and were performed and reported in compliance with ARRIVE guidelines (Animal Research: Reporting In Vivo Experiments). C57BL/6J male mice (3 months of age) were purchased from Jackson Laboratory (Bar Harbor, ME). All mice were maintained on a 12 h light/dark cycle with food and water provided ad libitum. CX-4945 and MS-275 were purchased from Cayman Chemical (Ann Harbor, ME). The sources of other chemicals used were described previously (Baltan et al., 2008 (link); Bastian et al., 2018 (link); Stahon et al., 2016 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!