7890a mass spectrometer

Manufactured by Agilent Technologies

Sourced in United States

The 7890A mass spectrometer is a highly sensitive analytical instrument designed for the detection and identification of chemical compounds. It utilizes advanced mass spectrometry technology to provide accurate and reliable measurements of molecular masses and their respective structures. The core function of the 7890A is to enable the precise analysis of complex samples, supporting a wide range of applications in various scientific and industrial fields.

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Lab products found in correlation

Tbdmcs, by Merck Group (1 mentions) 5975 gas chromatograph, by Agilent Technologies (1 mentions) Agilent 5975c msd, by Agilent Technologies (1 mentions) Db 5ms column, by Agilent Technologies (1 mentions)

5 protocols using 7890a mass spectrometer

GC-MS Analysis of Degradation Products

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Degraded products were
centrifuged at 15,000 rpm for 15 min and
subsequently filtered through 0.45 mm filters. A liquid–liquid
extraction was carried out using acetonitrile and NaCl, which were
used in equal volumes. GC–MS was performed with a 7890A mass
spectrometer (Agilent Technologies). The injection volume for analysis
was 1 mL The analytical column HP-5MS (30 mm × 0.25 mm, 0.25
μm) was used. The initial column temperature range was 80 °C
for 1.0 min, then it increased linearly at 5 °C/min to 250 °C
and held for 5 min. The helium carrier flow rate was 1 mL/min. The
chromatograph was equipped with a reverse-phase glacial acetic acid,
methanol, and ultra-pure water. Degradation products were identified
by mass spectra and their fragmentation pattern.

Baena-Baldiris D., Montes-Robledo A, & Baldiris-Avila R. (2020). Franconibacter sp., 1MS: A New Strain in Decolorization and Degradation of Azo Dyes Ponceau S Red and Methyl Orange. ACS Omega, 5(43), 28146-28157.

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Intracellular Metabolite Profiling via GC-MS

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Cells were scraped using methanol-water. An equivalent volume of chloroform was then added, and the aqueous phase was collected and evaporated under airflow for polar intracellular metabolite analysis. After dissolution in 50 μL of 2% methoxyamine hydrochloride in pyridine, the tert-butylmethylsilyl derivative was prepared by adding 30 μL of N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide (MBTSTFA) + 1% tert-butyldimethylchlorosilane (TBDMCS; Sigma) and incubating at 55 °C for 1 h [22 ]. We monitored the ion clusters around m/z 459 (carbons 1-6 of citrate, electron impact ionization), m/z 174 (carbons 1–3 of pyruvate, electron impact ionization) and m/z 418 (carbons 1–4 of aspartate, electron impact ionization).
Mass spectral data were obtained on a 7890A mass spectrometer coupled with a 5675C gas chromatograph (Agilent Technologies). The settings are as follows: GC inlet 230 °C, transfer line 280 °C, MS source 230 °C, MS quad 150 °C. An HP-5 capillary column (30 m length, 250 μm diameter, 0.25 μm film thickness) was used for analysis of all metabolites.

Selivanov V.A., Benito A., Miranda A., Aguilar E., Polat I.H., Centelles J.J., Jayaraman A., Lee P.W., Marin S, & Cascante M. (2017). MIDcor, an R-program for deciphering mass interferences in mass spectra of metabolites enriched in stable isotopes. BMC Bioinformatics, 18, 88.

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Hexane-Based Extraction and GC-MS Analysis of C16 Residues

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At the end of the cultivation period, the residual C₁₆ in each flask was extracted using 20 ml n-hexane in a separating funnel and then was analyzed using a 5975C gas chromatograph (GC) coupled with a 7890A mass spectrometer (MS) detector (Agilent Technologies, Santa Clara, CA, USA). Helium was applied as the carrier gas at a flow rate of 25 ml min⁻¹. The oven temperature was set to 100°C for 1 min and then increased to 260°C at a rate of 20°C min⁻¹ and held at that temperature for 5 min. The external standard method was utilized to obtain the C₁₆ concentrations in each sample. Afterward, the residual C₁₆ concentration data were processed in Origin8.5 to obtain the time courses of specific C₁₆ removal rates. The C₁₆ removal rate was calculated as the difference in the percentage of the residual C₁₆ concentration between the experimental and the control treatments in 0.7734 g liter⁻¹ C₁₆.

Hu B., Wang M., Geng S., Wen L., Wu M., Nie Y., Tang Y.Q, & Wu X.L. (2020). Metabolic Exchange with Non-Alkane-Consuming Pseudomonas stutzeri SLG510A3-8 Improves n-Alkane Biodegradation by the Alkane Degrader Dietzia sp. Strain DQ12-45-1b. Applied and Environmental Microbiology, 86(8), e02931-19.

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Kumquat Peel Essential Oil Composition

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Kumquat peel EO composition was determined using gas chromatography coupled with mass spectrometry (GC–MS) (7890A/5975C, Agilent, USA). An Agilent computerized system comprising a 5,975 gas chromatograph coupled with a 7890A mass spectrometer was used. Gas chromatography analyses were performed with the HP 5,975 gas chromatograph equipped with a FID detector and an HP‐5™ fused silica capillary column (30 m × 0.25 μm × 0.25 μm film thickness) using helium as the carrier gas (1.0 ml/min) at a splitting ratio of 1:20. The injector and detector temperature were 280°C and 250°C, respectively. The oven temperature was initially held at 60°C for 2 min, then linearly increased by 8°C/min until reaching 250°C, and then held for 20 min. Ionization was obtained by electronic impact under a potential of 70 eV, with an ion source temperature of 230°C and the quadrupole temperature of 150°C. The mass spectra were recorded on a selective quadrupolar type Hewlett‐Packard detector model 7890A. Identification of components was mainly based on the comparison of their GC Kovats retention indices (RI), determined with reference to an homologous series of C8–C40 n‐alkanes. GC retention times were also analyzed, and computer matching with the NIST 11 library and comparison of the fragmentation patterns with those reported in the research literature were also performed to ensure accuracy.

Yu F., Wan N., Zheng Q., Li Y., Yang M, & Wu Z. (2021). Effects of ultrasound and microwave pretreatments on hydrodistillation extraction of essential oils from Kumquat peel. Food Science & Nutrition, 9(5), 2372-2380.

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GC-MS Analysis of Summer Savory and Thyme Oils

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Chemical compositions of summer savory and thyme white essential oils were further determined using a gas chromatography (Agilent 7890A)-mass spectrometer (GC-MS; Agilent 5975C MSD, Agilent Technologies, Santa Clara, CA, USA) and DB-5MS column (30 m × 0.25 mm i.d., 0.25 μm film thickness, Agilent, CA, USA). The oven temperature program was the same as for gas chromatography-flame ionization detector (GC-FID) analysis. Helium was the carrier gas, and the flow rate was 1.0 mL/min. The effluence from the DB-5 MS column was introduced directly into mass spectrometer (MS) through a transfer line (280 °C). The ionization was by electron impact (70 eV, source temperature 230 °C). The scan range was 41–400 amu. Most compounds of summer savory and thyme white plant essential oils were tentatively identified by comparing the mass spectra of the peaks with authentic samples in the National Institute of Standards and Technology mass spectral (NIST MS) library.

Kim J.E., Lee J.E., Huh M.J., Lee S.C., Seo S.M., Kwon J.H, & Park I.K. (2019). Fumigant Antifungal Activity via Reactive Oxygen Species of Thymus vulgaris and Satureja hortensis Essential Oils and Constituents against Raffaelea quercus-mongolicae and Rhizoctonia solani. Biomolecules, 9(10), 561.

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