Biebrich scarlet

MycoFluor™ mycoplasma detection kits were purchased from Thermo Scientific (Waltham, MA, USA). The fetal bovine serum was from Gibco (Gibco, Sigma Aldrich Company Ltd., Waltham, MA, USA). Sodium dodecyl sulfate, Sodium Deoxycholate Monohydrate, Triton™ X-100 solution, Glutaraldehyde and Formaldehyde solution, Isopropyl and Ethyl Alcohol, Accutase cocktail, fluorescent dyes Calcein AM, Propidium iodide and Bisbenzimide Hoechst 33342, Culture media alfa-MEM and DMEM, L-glutamine, gentamicin sulfate, Alcian Blue 8GX, Biebrich scarlet, Fast Green FCF, Schiff’s reagent and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA or Gibco, Sigma-Aldrich Company Ltd., Waltham, MA, USA). Dioxidine^® (Hydroxymethylquinoxalindioxide, 10 mg/mL) was purchased from JSC “Valenta Pharm” (Moscow, Russia). Mayer’s hematoxylin, eosin H, Alizarin red S, toluidine blue, neutral buffered formalin, and other reagents for histological analysis were purchased from Labiko LLC (Saint Petersburg, Russia). All other reagents used were of analytical reagent quality.