The following chemicals were used: 2c [9 (link)], dimethyl sulfoxide (DMSO), Doxorubicin, MK2206, Torin1, Bafilomycin A1, Chloroquine, ABT263 (Sigma), Gemcitabine, XMD8-92, YKL-06-061, ABT199 (CSN Scientific), Selumetinib, TMP195, MKC3946 (MedChemExpress), SAHA (Cayman Chemicals), NKL54 [46 (link)].
Mkc3946
Manufactured by MedChemExpress
Sourced in United States
MKC3946 is a selective and potent inhibitor of CDK7, a key regulator of transcription and cell cycle progression. It demonstrates high specificity and inhibitory activity against CDK7 in biochemical and cellular assays.
Automatically generated - may contain errors
Lab products found in correlation
Selumetinib, by MedChemExpress (1 mentions)
Abt 263, by Merck Group (1 mentions)
Cyclin e1, by Proteintech (1 mentions)
Cyclind3, by Proteintech (1 mentions)
Gsk2606414, by MedChemExpress (1 mentions)
Cyclin dependent kinase 2, by Proteintech (1 mentions)
Cyclin dependent kinase 4, by Proteintech (1 mentions)
Bortezomib, by LC Laboratories (1 mentions)
β actin, by Proteintech (1 mentions)
Celltiter blue, by Promega (1 mentions)
Fluostar optima, by BMG Labtech (1 mentions)
Retro 2, by Merck Group (1 mentions)
Thiamet g, by MedChemExpress (1 mentions)
Limulus amoebocyte lysate assay, by Associates of Cape Cod (1 mentions)
Osmi 1, by Merck Group (1 mentions)
4 protocols using mkc3946
1
Multi-Compound Cytotoxicity Screening
2
Bortezomib and Small-Molecule Inhibitors in Cell Studies
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Bortezomib was purchased from LC Laboratories (Woburn, MA, USA). For in vitro studies, the drug was reconstituted in dimethyl sulfoxide (DMSO) at a stock concentration of 1 mM and stored at −80°C. The small‐molecule inhibitors MKC3946 and GSK2606414 were bought from MedChemExpress (Monmouth Junction, NJ, USA). The stock solution for MKC3946 and GSK2606414 was prepared in DMSO at a concentration of 10 mM and stored at −20°C. The antibodies against p21Cip1, p27Kip1, X‐box‐binding protein 1 (Xbp1s), activating transcription factor 6 (Atf6), 78 kDa glucose‐regulated protein (Grp78), C/EBP homologous protein (Chop), cyclin D3, cyclin E1, cyclin‐dependent kinase 2 (CDK2), cyclin‐dependent kinase 4 (CDK4) and β‐actin were obtained from Proteintech (Wuhan, Hubei, China), and the antibody against activating transcription factor 4 (Atf4) was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). All other chemicals were obtained from Sigma‐Aldrich (Burlington, MA, USA) unless otherwise specified.
3
Cell Viability Assay for Combination Therapy
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Cell viability assay was carried out using Cell Titer‐Blue® (Promega, Hawthorn) according to the manufacturer's instructions. Cells were plated at 5000 cells per well in a Corning Costar 96‐well plate. Cells were then treated with MKC‐3946 (10 μM, catalogue number HY‐19710, MedChemExpress), TMZ (50 μM, catalogue number S1237, Selleck Chem, Sapphire Bioscience) or MKC‐3946 + TMZ for 72 h. At the end of treatment, Cell Titer‐Blue® reagent was added into each well followed with detection of fluorescent signal by Fluostar Optima (BMG Labtech) with a 560/590 nm filter set.
4
Purification and Characterization of Shiga Toxins
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Purified Stx2a and Stx2amut, which harbors the triple mutation Y77S/E167Q/R170L in the enzymatic active site of the A‐subunit, were purchased from NIAID (NIH Biodefense and Emerging Infections Research Repository; BEI Resources, Manassas, VA, USA). Stx1a was purified from recombinant Stx1a‐expressing E. coli DH5α (pCKS112) by sequential ion exchange and immunoaffinity chromatography. The purity of Stx1a was determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), silver staining, and western blot analysis. The level of endotoxin contaminants was reduced to < 0.1 ng/ml using ActiClean Etox columns (Sterogene Bioseparations, Carlsbad, CA, USA). The degree of endotoxin contamination was assessed by a Limulus Amoebocyte lysate assay (Associates of Cape Cod, East Falmouth, MA, USA). OSMI‐1 was initially purchased from Sigma (SML 1621) and subsequently from Cayman (21894), and Retro‐2 was from Sigma (SML 1085). MKC‐3946 (HY‐19710) and Thiamet G (HY‐12588) were purchased from MedChemExpress.
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