Tsq quantum mass spectrometer

Manufactured by Thermo Fisher Scientific

Sourced in United States, Japan

The TSQ Quantum mass spectrometer is a triple quadrupole mass spectrometer designed for high-performance quantitative and qualitative analysis. It provides accurate and precise mass measurements to support a variety of analytical applications.

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Lab products found in correlation

Xcalibur 2, by Thermo Fisher Scientific (2 mentions) Vanquish uhplc system, by Thermo Fisher Scientific (2 mentions) Sil 20a autosampler, by Shimadzu (1 mentions) C18 security guard column, by Phenomenex (1 mentions) Lc 20ad series hplc system, by Shimadzu (1 mentions) Luna c18 column, by Phenomenex (1 mentions) Xcalibur software, by Thermo Fisher Scientific (1 mentions) Xterra c18 column, by Waters Corporation (1 mentions) Acquity uplc system, by Waters Corporation (1 mentions) J w vf 5ms column, by Agilent Technologies (1 mentions) Ultimate 3000 rs, by Thermo Fisher Scientific (1 mentions) Thermo trace gc ultra, by Thermo Fisher Scientific (1 mentions) Esi source, by Thermo Fisher Scientific (1 mentions) C18 column, by Phenomenex (1 mentions) Fluoview fv1000 laser scanning microscope, by Olympus (1 mentions) Fluoromax 4 luminescence spectrometer, by Horiba (1 mentions) Du800 spectrophotometer, by Beckman Coulter (1 mentions) Liquid chromatograph, by Thermo Fisher Scientific (1 mentions) Centrifuge 5417r, by Eppendorf (1 mentions) Zorbax eclipse xdb c18, by Agilent Technologies (1 mentions)

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22 protocols using tsq quantum mass spectrometer

Quantitative HPLC-MS Analysis of Emodin

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HPLC was performed on a Shimadzu LC-20AD series HPLC system (Kyoto, Japan) consisting of two LC-20AD pumps, a CTO-20A column oven (set at 35°C) and a SIL-20A autosampler. Analyte separation was achieved on a Phenomenex Luna C₁₈ column (150 × 2.00 mm, 5 μm) preceded by a Phenomenex C₁₈ security guard column, with an isocratic mobile phase consisting of 0.1% formic acid and methanol (20:80, v/v) at a flow rate of 0.3 mL/min.
MS detection was performed on a Thermo Finnigan TSQ quantum mass spectrometer (San Jose, CA, USA). Ultra-high pure nitrogen was used as the sheath and auxiliary gas. The ESI source was operated in negative ion mode under the following conditions: spray voltage, 3.5 kV; capillary temperature, 400°C; sheath gas, 35 arbitrary units; auxiliary gas, 15 arbitrary units. For quantification in multiple-reaction monitoring (MRM) cone voltage, collision energy and precursor to production transition m/z for emodin and IS were as shown in Table 1. For metabolism study of emodin, three phase II metabolites of emodin were monitored simultaneously using MRM mode (Teng et al., 2007 (link)).

Chen J., Li S., Liu M., Lam C.W., Li Z., Xu X., Chen Z., Zhang W, & Yao M. (2017). Bioconcentration and Metabolism of Emodin in Zebrafish Eleutheroembryos. Frontiers in Pharmacology, 8, 453.

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Quantitative Mass Spectrometry Analysis of Bile Acids

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MS analysis was performed using a TSQ Quantum mass spectrometer (ThermoFinnigan, Sunnydale, CA) equipped with an electrospray ionization (ESI) probe in negative-ion mode. Quantitation was done in a multiple reaction monitoring mode with collision energy of 10 V. The following (optimized) parameters were used for the detection of the analytes and the internal standard; N2 sheath gas, 49 p.s.i.; N2 auxiliary gas, 25 p.s.i.; spray voltage, 3.0 kV; source CID, 25 V; capillary temperature, 300 °C; capillary offset, −35 V; tube lens voltage, 160 V; Q2 gas pressure, 1.5 mtor; Q3 scan width 1 m/z; Q1/Q3 peak widths at half-maximum, 0.7 m/z. Calibration curves and concentration of individual bile acids were calculated by LCQuan 2.5.5 software (ThermoFinnigan). Concentrations of individual bile acids were calculated from peak area in the chromatogram detected with SRM relative to the appropriate internal standard. The composition and amount of bile acids in serum are reported in Supplementary Tables 1 and 2 and Supplementary Figs 6 and 7.

Flynn C.R., Albaugh V.L., Cai S., Cheung-Flynn J., Williams P.E., Brucker R.M., Bordenstein S.R., Guo Y., Wasserman D.H, & Abumrad N.N. (2015). Bile diversion to the distal small intestine has comparable metabolic benefits to bariatric surgery. Nature Communications, 6, 7715.

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Quantifying Nicotine on Surfaces

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Prescreened cotton rounds (100% cotton facial wipes) were wetted with 1.5 mL of 0.1% ascorbic acid and wiped over a 100 cm² area, typically a wooden door unlikely to be frequently cleaned.^{18 (link)} Samples were stored at –20°C in the dark until analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using electrospray ionisation (ESI) on a Thermo-Finnigan TSQ Quantum Mass Spectrometer. A description of the analytic methods for the measurement of nicotine on surfaces is provided in the online supplementary material. Nicotine was quantified against the deuterated internal standard, nicotine-d₄ (CDN Isotopes, Pointe-Claire, Quebec, Canada). Nicotine levels were reported as micrograms of nicotine per square metre of surface (μg/m²).

Matt G.E., Quintana P.J., Zakarian J.M., Hoh E., Hovell M.F., Mahabee-Gittens M., Watanabe K., Datuin K., Vue C, & Chatfield D.A. (2016). When smokers quit: exposure to nicotine and carcinogens persists from thirdhand smoke pollution. Tobacco control, 26(5), 548-556.

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Quantification of ZMC1 by LC-MS/MS

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Chromatographic separation of ZMC1 and internal standard was performed using an Xterra C₁₈ column, 50 × 4.6 mm, 3.5 μm (Waters, MA, USA) at a column temperature of 25 ºC. The flow rate used was 250 μL/min with mobile phase of 0.1% formic acid (mobile phase A) and acetonitrile with 0.1% formic acid (mobile phase B). Gradient linear elution was as follows: 0–5.5 min, 95–50% A; 5.5–6.0 min, 50–10% A; 6.0–8.0 min, 10–95% A; 8.0–10.0 min, 95% A. The retention times of ZMC1 and IS were 5.2 and 6.3 min respectively.
Quantitation of ZMC1 was performed using Thermo Electron TSQ Quantum mass spectrometer equipped with an electrospray ionization (ESI) source operated in positive ion mode, a Surveyor MS pump and a Surveyor autosampler (Thermo Electron, San Jose, CA, USA). MS/MS conditions were as follows: ion spray voltage, 5000 V; capillary temperature, 350°C; sheath gas pressure, 5 arbitrary units; Aux gas pressure, 30 arbitrary units; and collision pressure, 1.5 arbitrary units. ZMC1 and IS were detected in single reaction monitoring mode, m/z 235.2/178.0 (ZMC1) and m/z 231.0/150.0 (ABBT). Chromatographic data acquisition, peak integration and quantitation were performed using Xcalibur software (Thermo Electron, San Jose, CA, USA).

Lin H., Yu X., Eng O.S., Buckley B., Kong A.N., Bertino J.R., Carpizo D.R, & Gounder M.K. (2015). A sensitive liquid chromatography–mass spectrometry bioanalytical assay for a novel anticancer candidate – ZMC1. Biomedical chromatography : BMC, 29(11), 1708-1714.

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Lipidomic Analysis of Rat Muscle

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Rat muscle samples (~50 mg) were homogenised in 1 mL of Milli Q water using 2.8 mm ceramic beads in a OMNI Bead Ruptor Homogeniser (Omni International, Kennesaw, GA, USA) (5.65 m/s, 2 × 1 min). Lipids were extracted from the rat muscle homogenate and plasma samples (0.25 mL) using the modified Svennerholm and Fredman [46 (link)] extraction protocol as described by Norris et al. [47 (link)]. The final non-polar chloroform fraction containing the PLs was made to 5 mL in choloroform/methanol (2:1) and used for PL analysis. The analysis of PLs was performed on an ACQUITY UPLC system (Waters, Milford, MA, USA), equipped with an APS-2 Hypersil column (150 mm × 2.1 mm, 3 μm, Thermo Electron Corporation, Waltham, MA, USA), and interfaced to a TSQ Quantum mass spectrometer (Thermo Electron Corporation, Waltham, MA, USA) using a heated electrospray ionisation source as previously described [44]. The PLs were detected using precursor ion or neutral losses that occur to the PLs during fragmentation [48 (link)].

Markworth J.F., Durainayagam B., Figueiredo V.C., Liu K., Guan J., MacGibbon A.K., Fong B.Y., Fanning A.C., Rowan A., McJarrow P, & Cameron-Smith D. (2017). Dietary supplementation with bovine-derived milk fat globule membrane lipids promotes neuromuscular development in growing rats. Nutrition & Metabolism, 14, 9.

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GC-MS Analysis of Organic Compounds

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A Trace GC Ultra instrument (Thermo Fisher Scientific) was used with helium as carrier gas, in programmable temperature vaporization splitless injection mode at a flow rate of 1.5 mL/min. Injector temperature was 250 °C and a volume of 1 µL was injected. The instrument was equipped with a J&W VF-5MS column (45 m × 0.32-mm i.d., film thickness 0.4 µm, Agilent Technologies). The GC temperature program was as follows: time (min)/oven temperature (°C) 0/40, 1/40, 10/130, 15/280 and 20/280. The GC was coupled to a TSQ Quantum mass spectrometer (Thermo Fisher Scientific) operating in MRM mode in positive polarity with an electron energy of 70 eV. The solvent delay time was 4 min. Precursor ions were fragmented using argon as collision gas and a collision energy of 10 eV with monitoring transitions from m/z 125 to 99 and from m/z 99 to 81.

John H., van der Schans M.J., Koller M., Spruit H.E., Worek F., Thiermann H, & Noort D. (2017). Fatal sarin poisoning in Syria 2013: forensic verification within an international laboratory network. Forensic Toxicology, 36(1), 61-71.

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Quantification of Kidney Fatty Acid Metabolites

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Fatty acid metabolites in kidneys were measured, as described previously, with a slight modification [22 (link)–24 (link)]. High-performance liquid chromatography (HPLC) was combined with ESI–MS using a TSQ quantum mass spectrometer (Thermo Fisher Scientific K.K., Tokyo, Japan). HPLC was performed using a Luna 3u C18(2) 100Å LC column (100 × 2.0 mm, Phenomenex, Torrance, CA, USA) at 30°C. Samples were eluted in a mobile phase comprising acetonitrile–methanol (4:1, v/v) and water–acetic acid (100:0.1, v/v) in a 27:73 ratio for 5 min, ramped up to a 70:30 ratio after 15 min, to a 80:20 ratio after 25 min, held for 8 min, ramped up to 100:0 ratio after 35 min, and held for 10 min with flow rate of 0.1 mL/min. MS–MS analyses were conducted in negative ion mode, and fatty acid metabolites were detected and quantified by selected reaction monitoring (SRM). Conditions for the detection of each compound by SRM are listed (S1 Table). Peaks were selected and their areas were calculated using the Xcalibur 2.1 software (Thermo Fisher Scientific K.K.).

Katakura M., Hashimoto M., Inoue T., Mamun A.A., Tanabe Y., Arita M, & Shido O. (2015). Chronic Arachidonic Acid Administration Decreases Docosahexaenoic Acid- and Eicosapentaenoic Acid-Derived Metabolites in Kidneys of Aged Rats. PLoS ONE, 10(10), e0140884.

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Metabolomic analysis of bioreactor cultures

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For metabolomic analysis, two 1.0 mL samples were taken daily from each bioreactor condition and centrifuged at 300g for 5 minutes to separate the culture media from the cell pellet. Two hundred microliters of samples of the culture medium were then diluted 1:10 in LC‐MS grade methanol (Cat# 1.06035, Sigma). Macromolecules were pelleted by centrifugation at 13000 rpm for 5 minutes (Biofuge Pico, Sorvall) to extract the supernatant. The extracted supernatant was diluted 1:2 in cell culture grade water and stored at −20°C for 8 hours prior to running high‐performance liquid chromatography mass spectrometry (HPLC‐MS). Samples were analyzed using a Thermo Fisher Vanquish UHPLC system integrated with a Thermo Fisher TSQ Quantum mass spectrometer. Data were processed using MAVEN. Statistical analysis and visual representation of the data were done in R and MATLAB.

Borys B.S., So T., Colter J., Dang T., Roberts E.L., Revay T., Larijani L., Krawetz R., Lewis I., Argiropoulos B., Rancourt D.E., Jung S., Hashimura Y., Lee B, & Kallos M.S. (2020). Optimized serial expansion of human induced pluripotent stem cells using low‐density inoculation to generate clinically relevant quantities in vertical‐wheel bioreactors. Stem Cells Translational Medicine, 9(9), 1036-1052.

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LC-ESI-MS/MS Analysis of Antifungal Compounds

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Extract fractions displaying antifungal activity were analysed by LC-ESI-MS/MS. The analysis was conducted in a positive ion mode using a TSQ quantum mass spectrometer (Thermo Fisher Scientific, Tokyo, Japan). Flow injection analysis was used, with an isocratic mobile phase of 50% (v/v) MeOH in distilled water with 0.1% (v/v) formic acid. Mass peaks were selected and m/z values were calculated using Xcalibur 2.1 software (Thermo Fisher Scientific)^{15 (link)}.

Da X., Nishiyama Y., Tie D., Hein K.Z., Yamamoto O, & Morita E. (2019). Antifungal activity and mechanism of action of Ou-gon (Scutellaria root extract) components against pathogenic fungi. Scientific Reports, 9, 1683.

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Pharmacokinetic Study of Ruxolitinib in Mice

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Animal studies were performed under protocols approved by the Institutional Animal Care and Use Committee at OSU. Single dose pharmacokinetic studies were conducted in 8–12-week-old female and male NSG and BoyJ mice to determine a ruxolitinib dose that produces human equivalent exposure. For these studies, 60 mg kg⁻¹ of ruxolitinib was administered by oral gavage in 0.5% methylcellulose. Serial blood collection was performed from 15 min up to 240 min after dose administration. Total ruxolitinib concentrations in plasma were measured using a modification of previously published methods^{61 (link)}. Quantitation was carried out by liquid chromatography-tandem mass spectrometry with a Vanquish UHPLC system and a TSQ Quantum mass spectrometer (ThermoFisher Scientific); separation was achieved in 5 min using an Accucore Vanquish C18 column and the system was controlled using Thermo Trace Finder General Quan software.

Drenberg C.D., Shelat A., Dang J., Cotton A., Orwick S.J., Li M., Jeon J.Y., Fu Q., Buelow D.R., Pioso M., Hu S., Inaba H., Ribeiro R.C., Rubnitz J.E., Gruber T.A., Guy R.K, & Baker S.D. (2019). A high-throughput screen indicates gemcitabine and JAK inhibitors may be useful for treating pediatric AML. Nature Communications, 10, 2189.

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