Immunohistochemistry was performed on Formalin-Fixed, Paraffin Embedded (FFPE) slides using the Avidin/Biotinylated enzyme system (VECTASTAIN ABC, Vector Laboratories). Heat induced epitope retrieval was performed using a pressure cooker with Tris-EDTA Buffer. Samples were provided by the Biospecimen Core of the NYU IMCG. The number of melanoma samples was selected based on the homogeneity of HSF1 staining in nevi, primary and metastatic samples. No statistical method was used to predetermine sample size. No samples were excluded or randomized. The study protocol was approved by the NYU Institutional Review Committee (IRB). All NYU patients signed informed consent. The anti-HSF1 antibody (1:10000, Thermo Scientific, RT-629-PABX). The anti-FBXW7 antibody (1:2000, Abcam, 109617). The slides were reviewed and scored by IMCG pathologist (FD) according to the intensity (0-2) of the staining as well as distribution (focal <50%, diffuse ≥50%). The IMCG pathologist was blinded while scoring the samples. In addition, rabbit anti-human Ki67, clone Sp6 (1:400; Thermoscientific; MA1-90584), mouse anti-human Melan-A, clone A103 (1:75; Dako; M7196) and mouse anti-human Tyrosinase, clone T311 (1:100; Biocare Medical; 155) were used.
Ma1 90584
Manufactured by Agilent Technologies
The MA1-90584 is a laboratory instrument manufactured by Agilent Technologies. It is designed to perform specific functions within a laboratory setting. A detailed and unbiased description of the product's core function cannot be provided while maintaining conciseness and avoiding interpretation or extrapolation.
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2 protocols using ma1 90584
1
Immunohistochemistry Analysis of Melanoma Samples
2
Immunohistochemistry Analysis of Melanoma Samples
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Immunohistochemistry was performed on Formalin-Fixed, Paraffin Embedded (FFPE) slides using the Avidin/Biotinylated enzyme system (VECTASTAIN ABC, Vector Laboratories). Heat induced epitope retrieval was performed using a pressure cooker with Tris-EDTA Buffer. Samples were provided by the Biospecimen Core of the NYU IMCG. The number of melanoma samples was selected based on the homogeneity of HSF1 staining in nevi, primary and metastatic samples. No statistical method was used to predetermine sample size. No samples were excluded or randomized. The study protocol was approved by the NYU Institutional Review Committee (IRB). All NYU patients signed informed consent. The anti-HSF1 antibody (1:10000, Thermo Scientific, RT-629-PABX). The anti-FBXW7 antibody (1:2000, Abcam, 109617). The slides were reviewed and scored by IMCG pathologist (FD) according to the intensity (0-2) of the staining as well as distribution (focal <50%, diffuse ≥50%). The IMCG pathologist was blinded while scoring the samples. In addition, rabbit anti-human Ki67, clone Sp6 (1:400; Thermoscientific; MA1-90584), mouse anti-human Melan-A, clone A103 (1:75; Dako; M7196) and mouse anti-human Tyrosinase, clone T311 (1:100; Biocare Medical; 155) were used.
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