Pancreatin 8xusp
Manufactured by Merck Group
Sourced in Sao Tome and Principe, United Kingdom
Pancreatin 8XUSP is a laboratory-grade enzyme preparation derived from porcine pancreas. It is a complex mixture of digestive enzymes, including amylase, lipase, and protease, and is commonly used in research and analytical applications requiring enzymatic digestion or hydrolysis.
Automatically generated - may contain errors
Lab products found in correlation
Limonene oxide, by Merck Group (1 mentions)
Succinic acid, by Thermo Fisher Scientific (1 mentions)
Carvone, by Merck Group (1 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Ammonium hydroxide, by Thermo Fisher Scientific (1 mentions)
Gelatin type a, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)
Isopropanol, by Thermo Fisher Scientific (1 mentions)
D limonene, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Limonene, by Merck Group (1 mentions)
Potassium dihydrogen orthophosphate, by Merck Group (1 mentions)
Sodium hydroxide solution, by Avantor (1 mentions)
Hexane, by Thermo Fisher Scientific (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Hipersolv water, by Avantor (1 mentions)
β glucuronidase from helix pomatia type hp 2, by Merck Group (1 mentions)
2 protocols using pancreatin 8xusp
1
Encapsulation of D-Limonene in Alginate-Gelatin Complex
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D -Limonene, gelatin (type A with an isoelectric point of 7), n-hexane, pepsin, pancreatin 8XUSP were purchased from Sigma Aldrich (St. Louis, MO, United States). High viscosity sodium alginate (GRINDSTED Alginate FD 155) with pKa of 3.5 (Dupont Nutrition and Health, New Century, KS, United States) was selected for maximizing the extent of complex coacervation (16 , 24 (link)). Succinic acid, ammonium hydroxide, sodium hydroxide, sodium chloride and isopropanol were purchased from Fisher Scientific (Fair Lawn, NJ, United States). Anti-foam reagent was purchased from Spectrum Chemicals Mfg Corp (New Brunswick, NJ, United States). Aquacoat® ECD 30 (aqueous colloidal dispersion of ethylcellulose polymer-30% ethylcellulose stabilized by sodium lauryl sulfate (SLS) and cetyl alcohol) was provided by Colorcon (Harleysville, PA, United States) and tributyl citrate was purchased from TCI America (Portland, OR, United States). Limonene, carvone and Limonene oxide standards were purchased from Sigma Aldrich (St. Louis, MO, United States).
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2
Analysis of Paracetamol and Orthocetamol
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Ammonium acetate, potassium dihydrogen orthophosphate, β-glucuronidase from Helix pomatia (Type HP-2 at 100,000 units/ml) and pancreatin (8xUSP) were obtained from Sigma-Aldrich Company Ltd. (Dorset, UK). Acetonitrile, ethyl acetate, hexane and methanol were obtained from Fisher Scientific UK Ltd. (Loughborough, UK).
Optima grade formic acid was obtained from LGC (Middlesex, UK). Sodium hydroxide solution at 40% (v/v) and HiPerSolv water were obtained from VWR International Ltd.
(Lutterworth, UK). Water was purified using a Triple Red Duo Water system (Triple Red Laboratory Technology).
Paracetamol was purchased as powder from Sigma-Aldrich, whilst orthocetamol was purchased as powder from Toronto Research Chemicals (Canada). Deuterium labelled Paracetamol-d4 was purchased as powder from Cayman Chemicals (Cambridge, UK). Stock solutions were prepared at 2 mg/ml (Paracetamol and orthocetamol) and 1 mg/ml (Paracetamol-d4) in methanol and stored at -20ºC. Stock solutions of Paracetamol and orthocetamol were mixed 50:50 (v/v) to obtain a mixed standard at 1 mg/ml, which was subsequently diluted with methanol to obtain spiking solutions at appropriate concentrations.
Optima grade formic acid was obtained from LGC (Middlesex, UK). Sodium hydroxide solution at 40% (v/v) and HiPerSolv water were obtained from VWR International Ltd.
(Lutterworth, UK). Water was purified using a Triple Red Duo Water system (Triple Red Laboratory Technology).
Paracetamol was purchased as powder from Sigma-Aldrich, whilst orthocetamol was purchased as powder from Toronto Research Chemicals (Canada). Deuterium labelled Paracetamol-d4 was purchased as powder from Cayman Chemicals (Cambridge, UK). Stock solutions were prepared at 2 mg/ml (Paracetamol and orthocetamol) and 1 mg/ml (Paracetamol-d4) in methanol and stored at -20ºC. Stock solutions of Paracetamol and orthocetamol were mixed 50:50 (v/v) to obtain a mixed standard at 1 mg/ml, which was subsequently diluted with methanol to obtain spiking solutions at appropriate concentrations.
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