Mfp 3d bio instrument

Manufactured by Olympus

The MFP-3D-Bio is a high-resolution atomic force microscope (AFM) designed for biological applications. It provides three-dimensional topographical imaging and nanomechanical characterization of biological samples, such as cells, tissues, and biomolecules, at the nanoscale resolution.

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Lab products found in correlation

Ac240ts afm probe, by Olympus (3 mentions) Tr800psa cantilevers, by Olympus (1 mentions)

Close spelling variants of this product

MFP-3D-BIO MFP-3D

4 protocols using mfp 3d bio instrument

Visualizing DNA-SWCNT Complexes on Mica

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DNA-SWCNT complexes were plated on a freshly cleaved mica substrate (Structure Probe, Inc) for 4 min before washing with 10 ml of distilled water and blowing dry with argon gas. An Asylum Research MFP-3D-Bio instrument equipped with an Olympus AC240TS AFM probe in alternating current mode was used. Data were acquired at 2.93 nm pixel −1 x-y resolution and 15.63 pm of z resolution. The images were analyzed using Gwyddion software. To measure height or length distributions, at least 20 ROIs were analyzed.

Yaari Z., Yang Y., Apfelbaum E., Cupo C., Settle A.H., Cullen Q., Cai W., Roche K.L., Levine D.A., Fleisher M., Ramanathan L., Zheng M., Jagota A, & Heller D.A. (2021). A perception-based nanosensor platform to detect cancer biomarkers. Science Advances, 7(47), eabj0852.

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Reconstitution and Imaging of Lipid Bilayers

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Fresh DOPC:DOPS (1:1) and DOPC:DOPS (1:1), 10% (w/w) cholesterol SUVs were unrolled on freshly cleaved mica (∅ 1.2 cm) in HBS-Ca (10 mM HEPES, 150 mM NaCl, 2 mM CaCl₂, pH 7.5), by covering the mica entirely with 0.2 mg ml⁻¹ SUVs, incubating for 20 min at +30 °C, and washing twice with HBS-Ca.
Freshly prepared samples were imaged in HBS at ambient temperature using an Asylum Research MFP-3D Bio instrument and TR800PSA cantilevers (Olympus; spring constant (k) range 0.59–0.68 N m⁻¹, resonance frequency 77 kHz) in alternative current (AC) mode. Square 256 × 256 pixel scans were acquired from areas of 5–20 µm, with a 90° scanning angle and a scan speed of 0.6–0.8 Hz. The resulting scan images were processed with Igor Pro 6.37.
After confirming the presence of lipid bilayers, 0.5–3.6 µM MBP was added onto the bilayer samples in HBS. The samples were incubated for 15 min at ambient temperature, washed twice with HBS, and scanned as above. For every protein concentration, 2–6 samples were prepared and scanned with excellent reproducibility. Scans from at least 3 different areas for each sample were acquired to rule out artifacts originating from sample heterogeneity.

Raasakka A., Ruskamo S., Kowal J., Barker R., Baumann A., Martel A., Tuusa J., Myllykoski M., Bürck J., Ulrich A.S., Stahlberg H, & Kursula P. (2017). Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line. Scientific Reports, 7, 4974.

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Atomic Force Microscopy of miRNA Sensors

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The GT15mir19 sensor was incubated overnight at 20 mg/L with 10 uM of the miR-19-hairpin or 10 uM of the R23-hairpin in saline sodium citrate diluted 20x in 20 mM HEPES + 5 mM MgCl₂. The sample was plated on a freshly cleaved mica substrate (SPI) for 4 minutes before washing with 10 mL of dH2O and blowing dry with argon gas. An Asylum Research MFP-3D-Bio instrument was used with an Olympus AC240TS AFM probe in AC mode. Data was captured at 2.93 nm/pixel XY resolution and 15.63 pm Z resolution. For AFM under aqueous conditions, 20 mg/L of the GT15mir19 sensor was incubated with 10 uM of the miR-19-hairpin, R23-hairpin, or buffer overnight. All three conditions were spin-filtered 3x with 100 kDa Amicon centrifuge filters, and resuspended with 5 mM NiCl₂, 20 mM HEPES pH 6.7 buffer. The samples were plated onto freshly cleaved mica for 2 minutes before gently washing with the same buffer. Samples were imaged in a droplet of the buffer using an Asylum Research Cypher ES + BlueDrive AFM with an Olympus AC55 probe and imaged using BlueDrive excitation at the ambient temperature of 31°C within the AFM enclosure. All three samples were imaged with the same probe, consecutively, with the same scan settings, starting with the miR-19-hairpin sample, followed by the R23-hairpin control and the buffer control.

Harvey J.D., Jena P.V., Baker H.A., Zerze G.H., Williams R.M., Galassi T.V., Roxbury D., Mittal J, & Heller D.A. (2017). A Carbon Nanotube Reporter of miRNA Hybridization Events In Vivo. Nature biomedical engineering, 1, 0041.

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Atomic Force Microscopy of DNA-SWCNT Complexes

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DNA-SWCNT complexes were plated on a freshly cleaved mica substrate (SPI) for 4 minutes before washing with 10 ml of distilled water and blowing dry with argon gas. An Asylum Research MFP-3D-Bio instrument equipped with an Olympus AC240TS AFM probe in alternating-current mode was used. Data were acquired at 2.93 nm pixel -1 x-y resolution and 15.63 pm z resolution.
The images were analyzed using Gwyddion software. To measure height or length distributions, at least 20 ROIs were analyzed.

Yaari Z., Yang Y., Apfelbaum E., Settle A., Cullen Q., Cai W., Roche K.L., Levine D.A., Fleisher M., Ramanathan L.V., Zheng M., Jagota A., & Heller D.A. (2021). Machine-Perception Nanosensor Platform to Detect Cancer Biomarkers.

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